Effect of cryopreservation of peripheral blood mononuclear cells (PBMCs) on the variability of an antigen-specific memory B cell ELISpot

References

• Clutterbuck EA, Lazarus R, Yu L-M, Bowman J, Bateman EAL, Diggle L, Angus B, Peto TE, Beverley PC, Mant D, et al. Pneumococcal conjugate and plain polysaccharide vaccines have divergent effects on antigen-specific B cells. J Infect Dis 2012; 205:1408 - 16; http://dx.doi.org/10.1093/infdis/jis212; PMID: 22457293
   PubMed Web of Science ®Google Scholar
• Blanchard Rohner G, Snape MD, Kelly DF, John T, Morant A, Yu LM, Borkowski A, Ceddia F, Borrow R, Siegrist CA, et al. The magnitude of the antibody and memory B cell responses during priming with a protein-polysaccharide conjugate vaccine in human infants is associated with the persistence of antibody and the intensity of booster response. J Immunol 2008; 180:2165 - 73; http://dx.doi.org/10.4049/jimmunol.180.4.2165; PMID: 18250423
   PubMed Web of Science ®Google Scholar
• Blanchard-Rohner G, Pulickal AS, Jol-van der Zijde CM, Snape MD, Pollard AJ. Appearance of peripheral blood plasma cells and memory B cells in a primary and secondary immune response in humans. Blood 2009; 114:4998 - 5002; http://dx.doi.org/10.1182/blood-2009-03-211052; PMID: 19843885
   PubMed Web of Science ®Google Scholar
• Clarke ET, Williams NA, Findlow J, Borrow R, Heyderman RS, Finn A. Polysaccharide-specific memory B cells generated by conjugate vaccines in humans conform to the CD27+IgG+ isotype-switched memory B Cell phenotype and require contact-dependent signals from bystander T cells activated by bacterial proteins to differentiate into plasma cells. J Immunol 2013; 191:6071 - 83; http://dx.doi.org/10.4049/jimmunol.1203254; PMID: 24227777
   PubMed Web of Science ®Google Scholar
• Clarke ET, Williams NA, Dull PM, Findlow J, Borrow R, Finn A, Heyderman RS. Polysaccharide-protein conjugate vaccination induces antibody production but not sustained B-cell memory in the human nasopharyngeal mucosa. Mucosal Immunol 2013; 6:288 - 96; http://dx.doi.org/10.1038/mi.2012.70; PMID: 22806100
   PubMed Web of Science ®Google Scholar
• Mitchell R, Kelly DF, Pollard AJ, Trück J. Polysaccharide-specific B cell responses to vaccination in humans. Hum Vaccin Immunother 2014; 10:10; http://dx.doi.org/10.4161/hv.28350; PMID: 24632599
   PubMed Web of Science ®Google Scholar
• Kleeberger CA, Lyles RH, Margolick JB, Rinaldo CR, Phair JP, Giorgi JV. Viability and recovery of peripheral blood mononuclear cells cryopreserved for up to 12 years in a multicenter study. Clin Diagn Lab Immunol 1999; 6:14 - 9; PMID: 9874657
   PubMedGoogle Scholar
• Aziz N, Margolick JB, Detels R, Rinaldo CR, Phair J, Jamieson BD, Butch AW. Value of a quality assessment program in optimizing cryopreservation of peripheral blood mononuclear cells in a multicenter study. Clin Vaccine Immunol 2013; 20:590 - 5; http://dx.doi.org/10.1128/CVI.00693-12; PMID: 23408528
   PubMed Web of Science ®Google Scholar
• Kyu SY, Kobie J, Yang H, Zand MS, Topham DJ, Quataert SA, Sanz I, Lee FE. Frequencies of human influenza-specific antibody secreting cells or plasmablasts post vaccination from fresh and frozen peripheral blood mononuclear cells. J Immunol Methods 2009; 340:42 - 7; http://dx.doi.org/10.1016/j.jim.2008.09.025; PMID: 18996127
   PubMed Web of Science ®Google Scholar
• Costantini A, Mancini S, Giuliodoro S, Butini L, Regnery CM, Silvestri G, Montroni M. Effects of cryopreservation on lymphocyte immunophenotype and function. J Immunol Methods 2003; 278:145 - 55; http://dx.doi.org/10.1016/S0022-1759(03)00202-3; PMID: 12957403
   PubMed Web of Science ®Google Scholar
• Owen RE, Sinclair E, Emu B, Heitman JW, Hirschkorn DF, Epling CL, Tan QX, Custer B, Harris JM, Jacobson MA, et al. Loss of T cell responses following long-term cryopreservation. J Immunol Methods 2007; 326:93 - 115; http://dx.doi.org/10.1016/j.jim.2007.07.012; PMID: 17707394
   PubMed Web of Science ®Google Scholar
• Weinberg A, Song L-Y, Wilkening C, Sevin A, Blais B, Louzao R, Stein D, Defechereux P, Durand D, Riedel E, et al, Pediatric ACTG Cryopreservation Working Group. Optimization and limitations of use of cryopreserved peripheral blood mononuclear cells for functional and phenotypic T-cell characterization. Clin Vaccine Immunol 2009; 16:1176 - 86; http://dx.doi.org/10.1128/CVI.00342-08; PMID: 19515870
   PubMed Web of Science ®Google Scholar
• Weinberg A, Wohl DA, Brown DG, Pott GB, Zhang L, Ray MG, van der Horst C. Effect of cryopreservation on measurement of cytomegalovirus-specific cellular immune responses in HIV-infected patients. J Acquir Immune Defic Syndr 2000; 25:109 - 14; http://dx.doi.org/10.1097/00126334-200010010-00004; PMID: 11103040
   PubMed Web of Science ®Google Scholar
• Weinberg A, Betensky RA, Zhang L, Ray G. Effect of shipment, storage, anticoagulant, and cell separation on lymphocyte proliferation assays for human immunodeficiency virus-infected patients. Clin Diagn Lab Immunol 1998; 5:804 - 7; PMID: 9801338
   PubMedGoogle Scholar
• Kreher CR, Dittrich MT, Guerkov R, Boehm BO, Tary-Lehmann M. CD4+ and CD8+ cells in cryopreserved human PBMC maintain full functionality in cytokine ELISPOT assays. J Immunol Methods 2003; 278:79 - 93; http://dx.doi.org/10.1016/S0022-1759(03)00226-6; PMID: 12957398
   PubMed Web of Science ®Google Scholar
• Maecker HT, Moon J, Bhatia S, Ghanekar SA, Maino VC, Payne JK, Kuus-Reichel K, Chang JC, Summers A, Clay TM, et al. Impact of cryopreservation on tetramer, cytokine flow cytometry, and ELISPOT. BMC Immunol 2005; 6:17; http://dx.doi.org/10.1186/1471-2172-6-17; PMID: 16026627
   PubMed Web of Science ®Google Scholar
• Kierstead LS, Dubey S, Meyer B, Tobery TW, Mogg R, Fernandez VR, Long R, Guan L, Gaunt C, Collins K, et al. Enhanced rates and magnitude of immune responses detected against an HIV vaccine: effect of using an optimized process for isolating PBMC. AIDS Res Hum Retroviruses 2007; 23:86 - 92; http://dx.doi.org/10.1089/aid.2006.0129; PMID: 17263637
   PubMed Web of Science ®Google Scholar
• Germann A, Oh Y-J, Schmidt T, Schön U, Zimmermann H, von Briesen H. Temperature fluctuations during deep temperature cryopreservation reduce PBMC recovery, viability and T-cell function. Cryobiology 2013; 67:193 - 200; http://dx.doi.org/10.1016/j.cryobiol.2013.06.012; PMID: 23850825
   PubMed Web of Science ®Google Scholar
• Czerkinsky CC, Nilsson LA, Nygren H, Ouchterlony O, Tarkowski A. A solid-phase enzyme-linked immunospot (ELISPOT) assay for enumeration of specific antibody-secreting cells. J Immunol Methods 1983; 65:109 - 21; http://dx.doi.org/10.1016/0022-1759(83)90308-3; PMID: 6361139
   PubMed Web of Science ®Google Scholar
• Crotty S, Aubert RD, Glidewell J, Ahmed R. Tracking human antigen-specific memory B cells: a sensitive and generalized ELISPOT system. J Immunol Methods 2004; 286:111 - 22; http://dx.doi.org/10.1016/j.jim.2003.12.015; PMID: 15087226
   PubMed Web of Science ®Google Scholar
• Jahnmatz M, Kesa G, Netterlid E, Buisman A-M, Thorstensson R, Ahlborg N. Optimization of a human IgG B-cell ELISpot assay for the analysis of vaccine-induced B-cell responses. J Immunol Methods 2013; 391:50 - 9; http://dx.doi.org/10.1016/j.jim.2013.02.009; PMID: 23454005
   PubMed Web of Science ®Google Scholar
• Janetzki S, Britten CM. The impact of harmonization on ELISPOT assay performance. Methods Mol Biol 2012; 792:25 - 36; http://dx.doi.org/10.1007/978-1-61779-325-7_2; PMID: 21956498
   PubMedGoogle Scholar
• Cox JH, Ferrari G, Kalams SA, Lopaczynski W, Oden N, D’souza MP, Elispot Collaborative Study Group. Results of an ELISPOT proficiency panel conducted in 11 laboratories participating in international human immunodeficiency virus type 1 vaccine trials. AIDS Res Hum Retroviruses 2005; 21:68 - 81; http://dx.doi.org/10.1089/aid.2005.21.68; PMID: 15665646
   PubMed Web of Science ®Google Scholar
• Janetzki S, Panageas KS, Ben-Porat L, Boyer J, Britten CM, Clay TM, Kalos M, Maecker HT, Romero P, Yuan J, et al, Elispot Proficiency Panel of the CVC Immune Assay Working Group. Results and harmonization guidelines from two large-scale international Elispot proficiency panels conducted by the Cancer Vaccine Consortium (CVC/SVI). Cancer Immunol Immunother 2008; 57:303 - 15; http://dx.doi.org/10.1007/s00262-007-0380-6; PMID: 17721781
   PubMed Web of Science ®Google Scholar
• Janetzki S, Britten CM, Kalos M, Levitsky HI, Maecker HT, Melief CJM, Old LJ, Romero P, Hoos A, Davis MM. “MIATA”-minimal information about T cell assays. Immunity 2009; 31:527 - 8; http://dx.doi.org/10.1016/j.immuni.2009.09.007; PMID: 19833080
   PubMed Web of Science ®Google Scholar
• Zhang W, Caspell R, Karulin AY, Ahmad M, Haicheur N, Abdelsalam A, Johannesen K, Vignard V, Dudzik P, Georgakopoulou K, et al. ELISPOT assays provide reproducible results among different laboratories for T-cell immune monitoring--even in hands of ELISPOT-inexperienced investigators. J Immunotoxicol 2009; 6:227 - 34; http://dx.doi.org/10.3109/15476910903317546; PMID: 19908941
   PubMed Web of Science ®Google Scholar
• Clutterbuck EA, Oh S, Hamaluba M, Westcar S, Beverley PCL, Pollard AJ. Serotype-specific and age-dependent generation of pneumococcal polysaccharide-specific memory B-cell and antibody responses to immunization with a pneumococcal conjugate vaccine. Clin Vaccine Immunol 2008; 15:182 - 93; http://dx.doi.org/10.1128/CVI.00336-07; PMID: 18032593
   PubMed Web of Science ®Google Scholar
• Trück J, Lazarus R, Clutterbuck EA, Bowman J, Kibwana E, Bateman EAL, Pollard AJ. The zwitterionic type I Streptococcus pneumoniae polysaccharide does not induce memory B cell formation in humans. Immunobiology 2013; 218:368 - 72; http://dx.doi.org/10.1016/j.imbio.2012.05.008; PMID: 22704520
   PubMed Web of Science ®Google Scholar
• Strober W. Trypan blue exclusion test of cell viability. Curr Protoc Immunol. Appendix 2001; 3:Appendix 3B
   Google Scholar
• R Core Team. R Foundation for Statistical Computing. R Foundation for Statistical Computing 2013
   Google Scholar
• Wickham H. ggplot2: elegant graphics for data analysis. New York: Springer; 2009.
   Google Scholar
• Janetzki S, Schaed S, Blachere NEB, Ben-Porat L, Houghton AN, Panageas KS. Evaluation of Elispot assays: influence of method and operator on variability of results. J Immunol Methods 2004; 291:175 - 83; http://dx.doi.org/10.1016/j.jim.2004.06.008; PMID: 15345315
   PubMed Web of Science ®Google Scholar
• Weinberg A, Zhang L, Brown D, Erice A, Polsky B, Hirsch MS, Owens S, Lamb K. Viability and functional activity of cryopreserved mononuclear cells. Clin Diagn Lab Immunol 2000; 7:714 - 6; PMID: 10882680
   PubMedGoogle Scholar