Testin and actin are key molecular targets of adjudin, an anti-spermatogenic agent, in the testis

References

• Cheng CY, Mruk DD. The biology of spermatogenesis: the past, present and future. Philos Trans R Soc Lond B Biol Sci 2010; 365:1459 - 1463
   PubMed Web of Science ®Google Scholar
• Cheng CY, Mruk DD. New frontiers in non-hormonal male contraception. Contraception 2010; 82:476 - 482
   PubMed Web of Science ®Google Scholar
• Wang C, Swerdloff RS. Hormonal approaches to male contraception. Curr Opin Urol 2010; 20:520 - 524
   PubMed Web of Science ®Google Scholar
• Nieschlag E. Male hormonal contraception. Handb Exp Pharmacol 2010; 198:197 - 223
   PubMedGoogle Scholar
• O'Donnell L, Meachem SJ, Stanton PG, McLachlan RI. Neill JD. Endocrine regulation of spermatogenesis. Physiology of Reproduction 2006; Amsterdam Elsevier 1017 - 1069
   Google Scholar
• Cheng CY, Mruk DD, Silvestrini B, Bonanomi M, Wong CH, Siu MKY, et al. AF-2364 [1-(2,4-dichlorobenzyl)-1H-indazole-3-carbohydrazide] is a potential male contraceptive: a review of recent data. Contraception 2005; 72:251 - 261
   PubMed Web of Science ®Google Scholar
• Mruk DD, Wong CH, Silvestrini B, Cheng CY. A male contraceptive targeting germ cell adhesion. Nat Med 2006; 12:1323 - 1328
   PubMed Web of Science ®Google Scholar
• Cheng CY, Mruk DD. A local autocrine axis in the testes that regulates spermatogenesis. Nat Rev Endocrinol 2010; 6:380 - 395
   PubMed Web of Science ®Google Scholar
• Mruk DD, Cheng CY. Cell-cell interactions at the ectoplasmic specialization in the testis. Trends Endocrinol Metab 2004; 15:439 - 447
   PubMed Web of Science ®Google Scholar
• O'Donnell L, Nicholls PK, O'Bryan MK, McLachlan RI, Stanton PG. Spermiation: the process of sperm release. Spermatogenesis 2011; 1:14 - 35
   PubMedGoogle Scholar
• Mruk DD, Cheng CY. Sertoli-Sertoli and Sertoli-germ cell interactions and their significance in germ cell movement in the seminiferous epithelium during spermatogenesis. Endocr Rev 2004; 25:747 - 806
   PubMed Web of Science ®Google Scholar
• Toyama Y, Maekawa M, Yuasa S. Ectoplasmic specializations in the Sertoli cells: new vistas based on genetic defects and testicular toxicology. Anat Sci Int 2003; 78:1 - 16
   PubMedGoogle Scholar
• Vogl AW, Pfeiffer DC, Mulholland D, Kimel G, Guttman J. Unique and multifunctional adhesion junctions in the testis: ectoplasmic specializations. Arch Histol Cytol 2000; 63:1 - 15
   PubMed Web of Science ®Google Scholar
• Vogl AW, Vaid KS, Guttman JA. Cheng CY. The Sertoli cell cytoskeleton. Molecular Mechanisms in Spermatogenesis 2008; Austin Landes Bioscience/Springer Science + Business Media LLC 186 - 211
   Google Scholar
• Wolski KM, Perrault C, Tran-Son-Tay R, Cameron DF. Strength measurement of the Sertoli-spermatid junctional complex. J Androl 2005; 26:354 - 359
   PubMed Web of Science ®Google Scholar
• Lie PPY, Cheng CY, Mruk DD. The biology of the desmosome-like junction: a versatile anchoring junction and signal transducer in the seminiferous epithelium. Int Rev Cell Mol Biol 2011; 286:223 - 269
   PubMed Web of Science ®Google Scholar
• Yan HHN, Mruk DD, Cheng CY. Junction restructuring and spermatogenesis: the biology, regulation, and implication in male conraceptive development. Curr Top Dev Biol 2008; 80:57 - 92
   PubMed Web of Science ®Google Scholar
• Cheng CY, Mruk DD. An intracellular trafficking pathway in the seminiferous epithelium regulating spermatogenesis: a biochemical and molecular perspective. Crit Rev Biochem Mol Biol 2009; 44:245 - 263
   PubMed Web of Science ®Google Scholar
• Mruk DD, Silvestrini B, Cheng CY. Anchoring junctions as drug targets: role in contraceptive development. Pharmacol Rev 2008; 60:146 - 180
   PubMed Web of Science ®Google Scholar
• Siu MK, Cheng CY. Dynamic cross-talk between cells and the extracellular matrix in the testis. BioEssays 2004; 26:978 - 992
   PubMed Web of Science ®Google Scholar
• Yan HH, Mruk DD, Lee WM, Cheng CY. Ectoplasmic specialization: a friend or a foe of spermatogenesis?. BioEssays 2007; 29:36 - 48
   PubMed Web of Science ®Google Scholar
• Cheng CY, Bardin CW. Identification of two testosterone-responsive testicular proteins in Sertoli cell-enriched culture medium whose secretion is suppressed by cells of the intact seminiferous tubule. J Biol Chem 1987; 262:12768 - 12779
   PubMedGoogle Scholar
• Cheng CY, Grima J, Stahler MS, Lockshin RA, Bardin CW. Testins are structurally related Sertoli cell proteins whose secretion is tightly coupled to the presence of germ cells. J Biol Chem 1989; 264:21386 - 21393
   PubMed Web of Science ®Google Scholar
• Grima J, Zhu LJ, Zong SD, Catterall JF, Bardin CW, Cheng CY. Rat testin is a newly identified component of the junctional complexes in various tissues whose mRNA is predominantly expressed in the testis and ovary. Biol Reprod 1995; 52:340 - 355
   PubMed Web of Science ®Google Scholar
• Grima J, Zhu LJ, Cheng CY. Testin is tightly associated with testicular cell membrane upon its secretion by Sertoli cells whose steady-state mRNA level in the testis correlates with the turnover and integrity of inter-testicular cell junctions. J Biol Chem 1997; 272:6499 - 6509
   PubMed Web of Science ®Google Scholar
• Grima J, Wong CCS, Zhu LJ, Zong SD, Cheng CY. Testin secreted by Sertoli cells is associated with the cell surface and its expression correlates with the disruption of Sertoli-germ cell junctions but not the inter-Sertoli tight junction. J Biol Chem 1998; 273:21040 - 21053
   PubMed Web of Science ®Google Scholar
• Zong SD, Zhu LJ, Grima J, Aravindan GR, Bardin CW, Cheng CY. Cyclic and postnatal developmental changes of testin in the rat seminiferous epithelium—an immunohistochemical study. Biol Reprod 1994; 51:843 - 851
   PubMed Web of Science ®Google Scholar
• Cheng CY, Silvestrini B, Grima J, Mo MY, Zhu LJ, Johansson E, et al. Two new male contraceptives exert their effects by depleting germ cells prematurely from the testis. Biol Reprod 2001; 65:449 - 461
   PubMed Web of Science ®Google Scholar
• Xia W, Mruk DD, Lee WM, Cheng CY. Unraveling the molecular targets pertinent to junction restructuring events during spermatogenesis using the adjudin-induced germ cell depletion model. J Endocrinol 2007; 192:563 - 583
   PubMed Web of Science ®Google Scholar
• Mok KW, Mruk DD, Lie PPY, Lui WY, Cheng CY. Adjudin, a potential male contraceptive, exerts its effects locally in the seminiferous epithelium of mammalian testes. Reproduction 2011; 141:571 - 580
   PubMed Web of Science ®Google Scholar
• Cheng CY, Mruk DD. Harris JR, Graham JM, Rickwood D. Tight junction assembly. Cell Biology Protocols 2006; New York John Wiley 296 - 299
   Google Scholar
• Chen YM, Lee NPY, Mruk DD, Lee WM, Cheng CY. Fer kinase/Fer T and adherens junction dynamics in the testis: an in vitro and in vivo study. Biol Reprod 2003; 69:656 - 672
   PubMed Web of Science ®Google Scholar
• Wolski KM, Mruk DD, Cameron DF. The Sertolispermatid junctional complex adhesion strength is affected in vitro by adjudin. J Androl 2006; 27:790 - 794
   PubMed Web of Science ®Google Scholar
• Koskimies AI, Kormano M, Lahti A. A difference in the immunoglobulin content of seminiferous tubule fluid and rete testis fluid of the rat. J Reprod Fertil 1971; 27:463 - 465
   PubMedGoogle Scholar
• Setchell BP. Finn CA. Sermatogenesis. The Mammalian Testis 1978; New York Cornell University Press 181 - 232
   Google Scholar
• Mruk DD. New perspectives in non-hormonal male contraception. Trends Endocrinol Metab 2008; 19:57 - 64
   PubMed Web of Science ®Google Scholar
• Cheng CY, Mruk DD. Regulation of spermiogenesis, spermiation and blood-testis barrier dynamics: novel insights from studies on Eps8 and Arp3. Biochem J 2011; 435:553 - 562
   Google Scholar
• Mruk DD, Lau ASN. RAB13 participates in ectoplasmic specialization dynamics in the rat testis. Biol Reprod 2009; 80:590 - 601
   PubMed Web of Science ®Google Scholar
• Wong EWP, Mruk DD, Lee WM, Cheng CY. Par3/Par6 polarity complex coordinates apical ectoplasmic specialization and blood-testis barrier restructuring during spermatogenesis. Proc Natl Acad Sci USA 2008; 105:9657 - 9662
   PubMed Web of Science ®Google Scholar
• Mok KW, Mruk DD, Lee WM, Cheng CY. Spermatogonial stem cells alone are not sufficient to re-initiate spermatogenesis in the rat testis following adjudin-induced infertility. Int J Androl 2011; DOI: 101111/j1365-2605201001183x
   PubMed Web of Science ®Google Scholar
• Zong SD, Bardin CW, Phillips D, Cheng CY. Testins are localized to the junctional complexes of rat Sertoli and epididymal cells. Biol Reprod 1992; 47:568 - 572
   PubMed Web of Science ®Google Scholar
• Toyama Y, Maekawa M, Yuasa S. Ectoplasmic specializations in the Sertoli cell: new vistas based on genetic defects and testicular toxicology. Anat Sci Int 2003; 78:1 - 16
   PubMedGoogle Scholar
• Disanza A, Carlier MF, Stradal TEB, Didry D, Frittoli E, Confalonieri S, et al. Eps8 controls actin-based motility by capping the barbed ends of actin filaments. Nat Cell Biol 2004; 6:1180 - 1188
   PubMed Web of Science ®Google Scholar
• Higgs HN. There goes the neighbourhood: Eps8 joins the barbed-end crowd. Nat Cell Biol 2004; 6:1147 - 1149
   PubMed Web of Science ®Google Scholar
• Firat-Karalar EN, Welch MD. New mechanisms and functions of actin nucleation. Curr Opin Cell Biol 2011; 23:4 - 13
   Google Scholar
• Rottner K, Hanisch J, Campellone KG. WASH, WHAMM and JMY: regulation of Arp2/3 complex and beyond. Trends Cell Biol 2010; 20:650 - 661
   PubMed Web of Science ®Google Scholar
• Silvestrini B, Palazzo G, De Gregorio M. Lonidamine and related compounds. Prog Med Chem 1984; 21:111 - 135
   Google Scholar
• Tash JS, Attardi B, Hild SA, Chakrasali R, Jakkaraj SR, Georg GI. A novel potent indazole carboxylic acid derivative blocks spermatogenesis and is contraceptive in rats after a single oral dose. Biol Reprod 2008; 78:1127 - 1138
   PubMed Web of Science ®Google Scholar
• Santiemma V, Tullio G, Iapadre G, Fabbrini A. Effects of lonidamine on pituitary-gonadal axis in man. Oncology 1984; 41:53 - 55
   PubMed Web of Science ®Google Scholar
• Evans WK, Sheperd FA, Mullis B. Phase II evaluation of lonidamine in patients with advanced malignancy. Oncology 1984; 41:69 - 77
   PubMed Web of Science ®Google Scholar
• Grima J, Cheng CY. Testin induction: the role of cyclic 3′, ′-adenosine monophosphate/protein kinase A signaling in the regulation of basal and lonidamine-induced testin expression by rat Sertoli cells. Biol Reprod 2000; 63:1648 - 1660
   PubMed Web of Science ®Google Scholar
• Parson JT, Horwitz AR, Schwartz MA. Cell adhesion: integrating cytoskeletal dynamics and cellular tension. Nat Rev Mol Cell Biol 2010; 11:633 - 643
   PubMed Web of Science ®Google Scholar
• Tash JS, Chakrasali R, Jakkaraj SR, Hughes J, Smith SK, Hornbaker K, et al. Gamendazole, an orally active indazole carboxylic acid male contraceptive agent, targets HSP90AB1 (HSP90BHETA) and EEFA1 (eEF1A), and stimulates Il1a transcription in rat Sertoli cells. Biol Reprod 2008; 78:1139 - 1152
   PubMed Web of Science ®Google Scholar
• Cheng CY, Mather JP, Byer AL, Bardin CW. Identification of hormonally responsive proteins in primary Sertoli cell culture medium by anion-exchange high performance liquid chromatography. Endocrinology 1986; 118:480 - 488
   Google Scholar
• Mruk DD, Siu MKY, Conway AM, Lee NPY, Lau ASN, Cheng CY. Role of tissue inhibitor of metallo-proteases-1 in junction dynamics in the testis. J Androl 2003; 24:510 - 523
   PubMed Web of Science ®Google Scholar
• Orth JM. Proliferation of Sertoli cells in fetal and post-natal rats: a quantitative autoradiographic study. Anat Rec 1982; 203:485 - 492
   PubMedGoogle Scholar
• Li JCH, Lee WM, Mruk DD, Cheng CY. Regulation of Sertoli cell myotubularin (rMTM) expression by germ cells in vitro. J Androl 2001; 22:266 - 277
   PubMed Web of Science ®Google Scholar
• Lui WY, Lee WM, Cheng CY. Transforming growth factor-β3 regulates the dynamics of Sertoli cell tight junctions via the p38 mitogen-activated protein kinase pathway. Biol Reprod 2003; 68:1597 - 1612
   PubMed Web of Science ®Google Scholar
• Galdieri M, Ziparo E, Palombi F, Russo MA, Stefanini M. Pure Sertoli cell cultures: a new model for the study of somatic-germ cell interactions. J Androl 1981; 5:249 - 259
   Google Scholar
• Lee NPY, Mruk DD, Conway AM, Cheng CY. Zyxin, axin and Wiskott-Aldrich syndrome protein are adaptors that link the cadherin/catenin protein complex to the cytoskeleton at adherens junctions in the seminiferous epithelium of the rat testis. J Androl 2004; 25:200 - 215
   PubMed Web of Science ®Google Scholar
• Aravindan GR, Pineau C, Bardin CW, Cheng CY. Ability of trypsin in mimicking germ cell factors that affect Sertoli cell secretory function. J Cell Physiol 1996; 168:123 - 133
   PubMed Web of Science ®Google Scholar
• Ono S. Mechanism of depolymerization and severing of actin filaments and its significance in cytoskeletal dynamics. Int Rev Cytol 2007; 258:1 - 82
   PubMedGoogle Scholar
• Cooper HM, Paterson Y. Production of antibodies 2005; Hoboken John Wiley & Sons
   Google Scholar
• Cheng CY, Mathur PP, Grima J. Structural analysis of clusterin and its subunits in ram rete testis fluid. Biochemistry 1988; 27:4079 - 4088
   PubMedGoogle Scholar
• Page M, Thorpe R. Walker JM. Purification of IgG using DEAE-Sepharose chromatography. The Protein Protocols Handbook 2001; Totowa Humana Press, Inc. 987 - 988
   Google Scholar
• Page M, Thorpe R. Walker JM. Purification of IgG by precipitation with sodium sulfate or ammonium sulfate. The Protein Protocols Handbook 2001; Totowa Humana Press, Inc. 983 - 984
   Google Scholar
• Lau ASN, Mruk DD. Rab8B GTPase and junction dynamics in the testis. Endocrinology 2003; 144:1549 - 1563
   PubMed Web of Science ®Google Scholar
• Sarkar O, Xia W, Mruk DD. Adjudin-mediated junction restructuring in the seminiferous epithelium leads to displacement of soluble guanylate cyclase from adherens junctions. J Cell Physiol 2006; 208:175 - 187
   PubMed Web of Science ®Google Scholar