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Journal of Extracellular Vesicles Volume 7, 2018 - Issue 1
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Technical Report

Precipitation with polyethylene glycol followed by washing and pelleting by ultracentrifugation enriches extracellular vesicles from tissue culture supernatants in small and large scales

Anna-Kristin LudwigInstitute for Transfusion Medicine, University Hospital Essen, University of Duisburg-Essen, Essen, Germany;Institute of Physiological Chemistry, Faculty of Veterinary Medicine, Ludwig-Maximilians-University Munich, Munich, GermanyView further author information
,
Kyra De MiroschedjiInstitute for Transfusion Medicine, University Hospital Essen, University of Duisburg-Essen, Essen, GermanyView further author information
,
Thorsten R. DoeppnerInstitute for Transfusion Medicine, University Hospital Essen, University of Duisburg-Essen, Essen, Germany;Department of Neurology, University Hospital Essen, University of Duisburg-Essen, Essen, Germany;Department of Neurology, University Medical Center Goettingen, Goettingen, GermanyView further author information
,
Verena BörgerInstitute for Transfusion Medicine, University Hospital Essen, University of Duisburg-Essen, Essen, GermanyView further author information
,
Johannes RuesingInorganic Chemistry and Center for Nanointegration Duisburg-Essen (CeNIDE), University of Duisburg-Essen, Essen, GermanyView further author information
,
Vera RebmannInstitute for Transfusion Medicine, University Hospital Essen, University of Duisburg-Essen, Essen, GermanyView further author information
,
Stephan DurstInstitute for Transfusion Medicine, University Hospital Essen, University of Duisburg-Essen, Essen, GermanyView further author information
,
Sören JansenInstitute for Transfusion Medicine, University Hospital Essen, University of Duisburg-Essen, Essen, GermanyView further author information
,
Michel BremerInstitute for Transfusion Medicine, University Hospital Essen, University of Duisburg-Essen, Essen, GermanyView further author information
,
Elmar BehrmannDepartment of Structural Biology, Max-Planck-Institute for Physiology, Dortmund, Germany;Institute of Biochemistry - Structural Biochemistry, University of Cologne, Cologne, Germany;Max Planck Research Group Structural Dynamics of Proteins, Center of Advanced European Studies and Research (caesar), Bonn, GermanyView further author information
,
Bernhard B. SingerInstitute of Anatomy, University Hospital Essen, University of Duisburg-Essen, Essen, GermanyView further author information
,
Holger JastrowInstitute of Anatomy, University Hospital Essen, University of Duisburg-Essen, Essen, Germany;Electron Microscopy Unit, Imaging Center Essen, University Hospital Essen, University of Duisburg, Essen, GermanyView further author information
,
Jan Dominik KuhlmannDepartment of Gynecology and Obstetrics, Medical Faculty and University Hospital Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany;National Center for Tumor Diseases (NCT), Partner Site Dresden, Dresden, Germany;German Cancer Consortium (DKTK), Dresden and German Cancer Research Center (DKFZ), Heidelberg, GermanyView further author information
,
Fouzi El MagraouiBiomedical Research, Human Brain Proteomics II, Leibniz-Institut für Analytische Wissenschaften-ISAS, Dortmund, GermanyView further author information
,
Helmut E. MeyerBiomedical Research, Human Brain Proteomics II, Leibniz-Institut für Analytische Wissenschaften-ISAS, Dortmund, GermanyView further author information
,
Dirk M. HermannDepartment of Neurology, University Hospital Essen, University of Duisburg-Essen, Essen, GermanyView further author information
,
Bertram OpalkaDepartment of Hematology, West German Cancer Center, University Hospital Essen, University of Duisburg-Essen, Essen, GermanyView further author information
,
Stefan RaunserDepartment of Structural Biology, Max-Planck-Institute for Physiology, Dortmund, GermanyView further author information
,
Matthias EppleInorganic Chemistry and Center for Nanointegration Duisburg-Essen (CeNIDE), University of Duisburg-Essen, Essen, GermanyView further author information
,
Peter A. HornInstitute for Transfusion Medicine, University Hospital Essen, University of Duisburg-Essen, Essen, GermanyView further author information
&
Bernd GiebelInstitute for Transfusion Medicine, University Hospital Essen, University of Duisburg-Essen, Essen, GermanyCorrespondence[email protected]
View further author information
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Article: 1528109 | Received 25 Mar 2018, Accepted 21 Sep 2018, Published online: 17 Oct 2018

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