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mAbs Volume 10, 2018 - Issue 3
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A novel bicistronic gene design couples stable cell line selection with a fucose switch in a designer CHO host to produce native and afucosylated glycoform antibodies

Gargi RoyAntibody Discovery and Protein Engineering, MedImmune LLC, Gaithersburg, Maryland, United States of AmericaCorrespondence[email protected]
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Tom MartinAntibody Discovery and Protein Engineering, MedImmune LLC, Gaithersburg, Maryland, United States of AmericaORCID Iconhttp://orcid.org/0000-0002-7491-8274
ORCID Icon,
Arnita BarnesAntibody Discovery and Protein Engineering, MedImmune LLC, Gaithersburg, Maryland, United States of America
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Jihong WangAnalytical Biochemistry, MedImmune LLC, Gaithersburg, Maryland, United States of America
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Rod Brian JimenezAnalytical Biochemistry, MedImmune LLC, Gaithersburg, Maryland, United States of America
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Megan RiceAntibody Discovery and Protein Engineering, MedImmune LLC, Gaithersburg, Maryland, United States of America
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Lina LiCell Culture and Fermentation Sciences, MedImmune LLC, Gaithersburg, Maryland, United States of America
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Hui FengAntibody Discovery and Protein Engineering, MedImmune LLC, Gaithersburg, Maryland, United States of America
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Shu ZhangAntibody Discovery and Protein Engineering, MedImmune LLC, Gaithersburg, Maryland, United States of America
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Raghothama ChaerkadyAntibody Discovery and Protein Engineering, MedImmune LLC, Gaithersburg, Maryland, United States of America
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Herren WuAntibody Discovery and Protein Engineering, MedImmune LLC, Gaithersburg, Maryland, United States of America
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Marcello MarelliAntibody Discovery and Protein Engineering, MedImmune LLC, Gaithersburg, Maryland, United States of America
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Diane HattonCell Culture and Fermentation Sciences, Biopharmaceutical Development, MedImmune, Cambridge, United Kingdom
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Jie ZhuCell Culture and Fermentation Sciences, MedImmune LLC, Gaithersburg, Maryland, United States of America
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Michael A. BowenAntibody Discovery and Protein Engineering, MedImmune LLC, Gaithersburg, Maryland, United States of America
 show all
Pages 416-430 | Received 09 Oct 2017, Accepted 13 Jan 2018, Published online: 22 Feb 2018

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Gargi Roy, Jason Reier, Andrew Garcia, Tom Martin, Megan Rice, Jihong Wang, Meagan Prophet, Ronald Christie, William Dall’Acqua, Sanjeev Ahuja, Michael A Bowen & Marcello Marelli. (2020) Development of a high yielding expression platform for the introduction of non-natural amino acids in protein sequences. mAbs 12:1.
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Articles from other publishers (11)

Bhagyashree Bachhav, Jacopo de Rossi, Carlos D. Llanos & Laura Segatori. (2023) Cell factory engineering: Challenges and opportunities for synthetic biology applications. Biotechnology and Bioengineering 120:9, pages 2441-2459.
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Simon Joubert, Julie Guimond, Sylvie Perret, Félix Malenfant, S. Mehdy Elahi, Anne Marcil, Marie Parat, Michel Gilbert, Anne E.G. Lenferink, Jason Baardsnes & Yves Durocher. (2022) Production of afucosylated antibodies in CHO cells by coexpression of an anti‐FUT8 intrabody. Biotechnology and Bioengineering 119:8, pages 2206-2220.
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Pavlos Kotidis, Masue Marbiah, Roberto Donini, Itzcóatl A. Gómez, Ioscani Jimenez del Val, Stuart M. Haslam, Karen M. Polizzi & Cleo Kontoravdi. 2022. Glycosylation. Glycosylation 147 167 .
Roberto DoniniStuart M. HaslamCleo Kontoravdi. (2021) Glycoengineering Chinese hamster ovary cells: a short history. Biochemical Society Transactions 49:2, pages 915-931.
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Kayla Bastian, Emma Scott, David J. Elliott & Jennifer Munkley. (2021) FUT8 Alpha-(1,6)-Fucosyltransferase in Cancer. International Journal of Molecular Sciences 22:1, pages 455.
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Yoshiki Narimatsu, Christian Büll, Yen-Hsi Chen, Hans H. Wandall, Zhang Yang & Henrik Clausen. (2021) Genetic glycoengineering in mammalian cells. Journal of Biological Chemistry 296, pages 100448.
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Itzcóatl Gómez Aquino & Ioscani Jiménez del Val. 2021. Cell Culture Engineering and Technology. Cell Culture Engineering and Technology 147 188 .
Isabelle Solomon, Maria Amann, Anne Goubier, Frederick Arce Vargas, Dimitrios Zervas, Chen Qing, Jake Y. Henry, Ehsan Ghorani, Ayse U. Akarca, Teresa Marafioti, Anna Śledzińska, Mariana Werner Sunderland, Dafne Franz Demane, Joanne Ruth Clancy, Andrew Georgiou, Josephine Salimu, Pascal Merchiers, Mark Adrian Brown, Reto Flury, Jan Eckmann, Claudio Murgia, Johannes Sam, Bjoern Jacobsen, Estelle Marrer-Berger, Christophe Boetsch, Sara Belli, Lea Leibrock, Joerg Benz, Hans Koll, Roger Sutmuller, Karl S. Peggs & Sergio A. Quezada. (2020) CD25-Treg-depleting antibodies preserving IL-2 signaling on effector T cells enhance effector activation and antitumor immunity. Nature Cancer 1:12, pages 1153-1166.
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Lynda J. Donald, Maureen Spearman, Neha Mishra, Emy Komatsu, Michael ButlerHélène Perreault. (2020) Mass spectrometric analysis of core fucosylation and sequence variation in a human–camelid monoclonal antibody. Molecular Omics 16:3, pages 221-230.
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Jonathan Sjögren, Rolf Lood & Andreas Nägeli. (2020) On enzymatic remodeling of IgG glycosylation; unique tools with broad applications. Glycobiology 30:4, pages 254-267.
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Linde Van Landuyt, Chiara Lonigro, Leander Meuris & Nico Callewaert. (2019) Customized protein glycosylation to improve biopharmaceutical function and targeting. Current Opinion in Biotechnology 60, pages 17-28.
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