SNP-Based Kompetitive Allele Specific PCR (KASP_TM) Method for the Qualification and Quantification of Hop Varieties

Abstract

There are several studies focused on single nucleotide polymorphisms (SNPs) of hops. However, to date, no feasible method for the qualification and quantification of hop varieties had been developed. A novel Kompetitive Allele Specific PCR (KASP) methodology for the qualification and quantification of hop varieties has been developed. One hundred and fifty gauge of high-throughput sequencing data were obtained from the ten hop varieties most commonly used in the Chinese brewing industry. After bioinformatic analysis, 2046 SNPs from sixteen hop varieties were selected from two million original SNPs for further confirmation by Sanger sequencing. Twelve SNP markers were successfully screened for the characterization and quantification of the mixed sample. The SNP markers and detection method developed provide potential means for the qualification and quantification of hop varieties in laboratories utilizing SNP marker technology.

Keywords:

• Hop varieties
• KASP
• qualification
• quantification
• SNP

Acknowledgments

This research was supported and funded by the CNRIFFI Science and Technology Development fund 2017-Brewing-505.

Disclosure statement

No potential conflict of interest was reported by the author(s).