Evaluation of Probiotic Potential of Lactic Acid Bacteria Isolated from Different Sources in Western India

ABSTRACT

Lactic acid bacteria isolated from unconventional sources are often attractive targets in the quest for obtaining better probiotics. In the present study, 16 members of the genus Lactobacillus, isolated from 3 different sources in western India, viz., plants, fermented foods and beverages, and human feces, were evaluated for their probiotic and bioactive properties. The isolates were closely related to Lactobacillus fermentum, Lactobacillus pentosus, and mainly Lactobacillus plantarum. The isolates were tolerant to bile salt, acidic pH and pancreatin, although pancreatin tolerance was generally low. Cellular extracts of several isolates displayed antioxidant activity, while cell-free supernatants displayed antibacterial activity against human pathogens. Antioxidant activity of Lactobacilli of human origin was higher than those from vegetables or fermented foods and beverages. L. plantarum AG40V prevented spoilage of fresh-cut fruits, vegetables and sprouted mung-beans. Lactobacilli from all sources displayed equal probiotic potential and those of human origin displayed superior antioxidant activity over others.

KEYWORDS:

• Lactic acid bacteria
• probiotics
• antibacterial activity
• antioxidant activity
• comet assay

Additional information

Funding

This study was partly supported by Dr. D.Y. Patil Vidyapeeth, Pune, India, through grant number DPU/15/2016 and by collaborating universities, University of Skövde, Sweden and Örebro University, Sweden.