4,586
Views
24
CrossRef citations to date
0
Altmetric
Articles

Simultaneous Determination of Nitrite and Nitrate in Milk Samples by Ion Chromatography Method and Estimation of Dietary Intake

, , , , , & show all
Pages 1983-1993 | Received 04 Jun 2015, Accepted 02 Sep 2015, Published online: 24 May 2016
 

Abstract

The presence of nitrate and nitrite in foods may be considered hazardous after ingestion in the gastrointestinal tract due to their reaction with naturally occurred secondary amines to form potentially carcinogenic nitrosamines. Due to this fact, a new method was developed in this study for the simultaneous determination of nitrite and nitrate in milk samples using by ion chromatography. Proposed mobile phase composed of sodium hydrogen carbonate and sodium carbonate (1.0 and 3.2 mmol/L) with a flow rate of 0.7 ml/min. The average recoveries for nitrate and nitrite were higher than 86 and 88%, respectively. The limit of detection for nitrate and nitrite were 0.24 and 0.09 mg/L, respectively. The results of 102 real milk samples showed nitrate was found in all of the samples (100%) with a mean of 34 ± 11 mg/L, while nitrite was found in none of the samples. The mean intake of nitrate in all age groups was lower than World Health Organization guideline. The present assessment concludes that the maximum contaminant level was equal to 82.8 mg/L nitrate. This method was fast, sensitive and accurate and is capable of being an alternative method in food control laboratories for investigation of nitrite and nitrate content. This is the first study of the determination and survey of nitrite and nitrate and exposure assessment of the Iranian population to nitrite and nitrate level in milk, which was widely used in infants and adolescents as one of the basic food components.

FUNDING

This work was supported by the deputy of research of Zanjan University of Medical Sciences (Grant NO: A-11-769-1). The results presented in this article were partly extracted from the thesis of Mr. Chamandust (MSc graduate of School of public health, Zanjan University of Medical Sciences) who performed his thesis under supervision of Dr. Mir-Jamal Hosseini in Faculty of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran.

Additional information

Funding

This work was supported by the deputy of research of Zanjan University of Medical Sciences (Grant NO: A-11-769-1). The results presented in this article were partly extracted from the thesis of Mr. Chamandust (MSc graduate of School of public health, Zanjan University of Medical Sciences) who performed his thesis under supervision of Dr. Mir-Jamal Hosseini in Faculty of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.