1,468
Views
8
CrossRef citations to date
0
Altmetric
Original Articles

A species specific simplex polymerase chain reaction-based approach for detection of goat tallow in heat clarified milk fat (ghee)

, , &
Pages S69-S75 | Received 29 Sep 2016, Accepted 29 Jan 2017, Published online: 25 Apr 2017
 

ABSTRACT

The detection of milk fat adulteration with foreign fats/oils continues to be a challenge for the dairy industry as well as food testing laboratories. Recently, various chromatographic methods have been developed to detect a low level of vegetable oil in ghee but detection of animal body fat in ghee is still a challenge. In India, goat body fat (goat tallow) adulteration in ghee is a very serious problem. In the present investigation, a rapid species specific polymerase chain reaction (PCR)-based methodology has been standardised to detect goat tallow in heat clarified milk fat (ghee). This standardised PCR-based protocol did not show any false positive results in the genuine ghee (heat clarified butter fat) samples. Adulteration of ghee (both cow and buffalo) with goat tallow at the 10% level could be detected using the standardised PCR-based protocol. The protocol standardised is rapid and convenient to use.

Acknowledgement

The authors are very much thankful to the Directors of ICAR-NDRI, Karnal and ICAR-NBAGR, Karnal for providing facilities to carry out this research.

Funding

The authors are very much thankful to the Ministry of Food Processing-Government of India (MOFPI) for providing financial assistance.

Additional information

Funding

The authors are very much thankful to the Ministry of Food Processing-Government of India (MOFPI) for providing financial assistance.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.