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ABSTRACT
In the present study, protein complexes emerged in high molecular weight (MW) region (95–170 kDa) were observed in fertilised chicken egg white after 7 days of incubation. Investigations were performed to reveal the possible proteins involved in these complexes. The emerged 10 protein bands from SDS-PAGE gel were confirmed to be ovalbumin through matrix-assisted laser desorption/ionisation time-of-flight tandem mass spectrometry (MALDI-TOF MS/MS) followed by Western blot analysis. To reveal the possible interactions among ovalbumin and other egg white proteins, LC connected to LTQ-Orbitrap mass spectrometer was used. Besides ovalbumin, 11 egg white proteins were identified including ovotransferrin, lysozyme, ovalbumin-related protein X, ovalbumin-related protein Y, hep21, alpha-enolase, ovoinhibitor, and a protein similar to ryanodine receptor 1. These results indicated widespread thermal-induced protein interactions among ovalbumin and other egg white proteins.
Funding
This work was financially supported by Science and Technology Program of Wuhan (2016020101010079), the Chinese National Natural Science Funds (Grant No. 31101366), the Special Fund for Agro-scientific Research in the Public Interest (201303084), and the Fundamental Research Funds for the Central Universities (2662017PY096).
Supplemental material
Supplemental data for this article can be accessed on the publisher’s website.
