Bioactive compounds and antioxidant activity of wheat bran and barley husk in the extracts with different polarity

ABSTRACT

Extracts of wheat bran and barley husk with solvents of different polarity were analyzed to determine the concentration of bioactive compounds (phytic acid, saponins, flavonoids, total phenolic compounds and phenolic profile) and their antioxidant activity by DPPH and ABTS assays. The results showed that the phytic acid concentration in wheat bran was higher than the phytic acid concentration in barley husk. The concentration of saponins was inverse with higher concentrations in barley husk than wheat bran. The solvents with the highest extraction of phytochemicals were 80% ethanol in wheat bran and 50% acetone in barley husk. The chromatograph profile showed that wheat bran presented a greater concentration of caffeic acid and barley husk contained kampferol. The properties of extraction solvents significantly affected the concentration of flavonoids, phenolic compounds, and antioxidant activity. The addition of water to the solvents increased the extraction efficiency of bioactive compounds and antioxidant activity. The results for barley husk are among the first reported because there are few studies on this cereal.

KEYWORDS:

• Wheat bran
• barley husk
• saponins
• phytic acid
• phenolic compounds
• antioxidant activity

Acknowledgments

This research manuscript was supported by Catedras CONACyT project 1232. In addition, the authors gratefully acknowledge María Yolanda Mercedes Rodríguez Aza from CINVESTAV- Irapuato Campus for her assistance with HPLC analysis. All authors possess no conflicts of interest.