Preparation and structural properties of amylose complexes with quercetin and their preliminary evaluation in delivery application

ABSTRACT

In this study, amylose, known as the food-grade biopolymers has been proved as a feasible solution for the controlled delivery of quercetin. The effect of reaction variables including reaction temperature, time, pH and quercetin addition amount on the content and conversion rate of quercetin was analyzed. Meanwhile, fourier transform infrared spectroscopy (FT-IR) and X-ray diffraction (XRD) provided evidence of quercetin complexation with amylose. Then, the molecular, cluster, and fractal structures of the complexes were investigated using ¹³C cross-polarization/magic angle spinning nuclear magnetic resonance (¹³C CP/MAS NMR) and small-angle X-ray scattering (SAXS). Furthermore, laser scanning confocal microscopy (CLSM) identified the precise encapsulation structure from direct observation and scanning electron microscope (SEM) was conducted to show that complexes displayed a larger size granule with smooth and angular appearance. From the antioxidant study, amylose/quercetin complexes presented effective scavenging capacity toward DPPH•. Besides, complexes showed high retention of quercetin in simulated stomach conditions but gradual release in simulated small intestine conditions. It was therefore confirmed that the amylose/quercetin complexes could be served as suitable carriers for the controlled release of quercetin in the digestive tract. The present research expanded the application range of starch, meanwhile proved an alternative application prospect for the ameliorative bioactive quercetin products.

Graphical Abstract

KEYWORDS:

• Amylose
• Quercetin
• complexes
• preparation
• characterization

Acknowledgments

This research was supported by the National Natural Science Foundation of China (21878106 and 21576098), the 111 Project (B17018), the Key Project of Science and Technology of Guangdong Province (2018A050506016 and 2017B090901002), the Key Project of Science and Technology of Guangzhou Economic Development Zone (2018GH21), the Key Project of Science and Technology of Guangzhou (201904010240), Fundamental Research Funds for the Central Universities, SCUT (2018KZ08). The authors declare no conflicts of interest exist in the submission of this manuscript. This article does not contain any studies with human or animal subjects.

Additional information

Funding

This work was supported by the National Natural Science Foundation of China [21576098,21878106];Fundamental Research Funds for the Central Universities, SCUT [2018KZ08];Key Project of Science and Technology of Guangzhou Economic Development Zone [2018GH21];Key Project of Science and Technology of Guangdong Province [2018A050506016 and 2017B090901002];Key Project of Science and Technology of Guangzhou [201904010240];111 Project [B17018].