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Articles

Optimized ultrasonic-assisted extraction of polysaccharides from Cyclina sinensis and evaluation of antioxidant activities in vitro

Extracción optimizada asistida por ultrasonidos de polisacáridos de Cyclina sinensis y evaluación de actividades antioxidantes in vitro

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Pages 32-39 | Received 14 Nov 2012, Accepted 10 Mar 2013, Published online: 04 Jun 2013
 

Abstract

The Box-Behnken design combined with response surface methodology was used to optimize ultrasonic-assisted extraction of polysaccharides from Cyclina sinensis. A high-extraction yield of polysaccharides, 34.63 ± 1.52 g/kg using an ultrasonic power of 500 W, liquid to material ratio of 22:1 (mL/g), with an extraction temperature of 77°C for 31 min was achieved. The polysaccharides exhibited quite a strong reducing property, the total antioxidant capacity, as well as excellent inhibitions of DPPH radical, hydroxyl radical, and superoxide radical. Meanwhile, it was found that the bioactivities appeared to be dose-dependent on polysaccharides.

Resumen: El diseño de Box-Behnken combinado con metodología de superficie de respuesta se utilizó para optimizar la extracción asistida por ultrasonidos de polisacáridos de Cyclina sinensis. Se logró un alto rendimiento de extracción de polisacáridos, 34,63 ± 1,52 g/kg utilizando energía ultrasónica de 500 W, relación de líquido a materiales de 22:01 (ml/g), con una temperatura de extracción de 77°C durante 31 min. Los polisacáridos mostraron una fuerte propiedad reductora, la capacidad antioxidante total, así como excelentes inhibiciones de radical DPPH, radical hidroxilo y radicales superóxido. Mientras tanto, se encontró que las bioactividades parecía ser dependiente de la dosis de polisacáridos.

Acknowledgments

This study was supported by the foundation for Science Research Project of Lianyungang (CG1131) and the Natural Science Research Program of Jiangsu Higher Education Department (10KJB350001).

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