Phenolic profiles, antioxidant and antimutagenic activities of Solanum lycopersicum var. cerasiforme accessions from Mexico

ABSTRACT

The fruit of 18 Solanum lycopersicum var. cerasiforme accessions from Mexico were evaluated for total phenolics (TP) by the Folin-Ciocalteau assay, phenolic profiles by high performance liquid chromatography-diode array detection-mass spectrometry (HPLC-DAD-MS), antioxidant activity (AoxA) by 2,2´-azino-bis(3-ethylbenzothiazolin)-6-sulfonic acid (ABTS), 2,2-diphenil-1-pycrilhydrazyl (DPPH), and oxygen radical absorbance capacity (ORAC), and antimutagenic activity (AmuA) by the Ames assay. TP was measured as Gallic Acid Equivalents (GAE) and the AoxA as Trolox Equivalents (TE). TP varied from 37 to 86 mg GAE 100 g⁻¹ fresh weight (fw). The AoxA by ABTS (568-1187 µmol TE 100 g⁻¹ fw) and DPPH (157-350 µmol TE 100 g⁻¹ fw) correlated with TP and the levels of caffeoylquinic acids and rutin. The AmuA did not correlate with the levels of phenolics. Some accessions had higher AoxA and AmuA than those reported for commercial cultivars and also showed high levels of caffeoylquinic acids and rutin; thus, their consumption could have good health promoting effects.

RESUMEN

En frutos de 18 accesiones de Solanum lycopersicum var. cerasiforme de México se evaluó contenido de fenólicos totales (FT) por el método de Folin-Ciocalteau, perfil de fenólicos por HPLC-DAD-MS, actividades antioxidante (AAox) por ABTS, DPPH y ORAC, y antimutagénica (AAmu) por el ensayo de Ames. Los FT se midieron como Equivalentes de Ácido Gálico (EAG) y la AAox como Equivalentes de Trolox (ET). Los FT variaron de 37 a 86 mg EAG 100 g⁻¹ peso fresco (pf). Las AAox por ABTS (568-1187 µmol ET 100 g⁻¹ pf) y DPPH (157-350 µmol ET 100 g⁻¹ pf) correlacionaron con el contenido de FT, ácidos cafeoilquínicos y rutina. La AAmu no correlacionó con los niveles de fenólicos. Algunas accesiones presentaron AAox y AAmu mayores que las reportadas para cultivares comerciales y mostraron altos niveles de ácidos cafeoilquínicos y rutina, por lo que su consumo podría tener buenos efectos de promoción de la salud.

KEYWORDS:

• Tomato
• polyphenols
• antioxidants
• antimutagens
• HPLC-MS

PALABRAS CLAVE:

• Tomate
• polifenoles
• antioxidantes
• antimutágenos
• HPLC-MS

Disclosure statement

No potential conflict of interest was reported by the authors.

Acknowledgment

Authors thank Laura Contreras (CIAD, AC, Culiacán, Sinaloa) for her technical assistance.

Additional information

Funding

This work was supported by Universidad Autónoma de Sinaloa [grants PROFAPI2012/092 and PROFAPI2013/102].