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Research Paper

Streptococcus mutans PrsA mediates AtlA secretion contributing to extracellular DNA release and biofilm formation in the pathogenesis of infective endocarditis

, , , , , , , , & ORCID Icon show all
Pages 1379-1392 | Received 18 Oct 2021, Accepted 20 Jul 2022, Published online: 11 Aug 2022
 

ABSTRACT

The role of secretion chaperone-regulated virulence proteins in the pathogenesis of infective endocarditis (IE) induced by viridans streptococci such as Streptococcus mutans is unclear. In this study, we investigated the contribution of the foldase protein PrsA, a putative parvulin-type peptidyl-prolyl isomerase, to the pathogenesis of S. mutans-induced IE. We found that a prsA-deficient strain had reduced virulence in terms of formation of vegetation on damaged heart valves, as well as reduced autolysis activity, eDNA release and biofilm formation capacity. The secretion and surface exposure of AtlA in vitro was reduced in the prsA-deficient mutant strain, and complementation of recombinant AtlA in the culture medium restored a wild type biofilm phenotype of the prsA-deficient mutant strain. This result suggests that secretion and surface localization of AtlA is regulated by PrsA during biofilm formation. Together, these results demonstrate that S. mutans PrsA could regulate AtlA-mediated eDNA release to contribute to biofilm formation in the pathogenesis of IE.

Acknowledgments

This study was supported by the Ministry of Science and Technology (111-2320-B-038-063, 111-2320-B-002-066, 111-2320-B-002-076 and 108-2320-B-038-057-MY3).

Disclosure statement

No potential conflict of interest was reported by the author(s).

Data availability statement

The authors confirm that the data supporting the findings of this study are available within the article and its supplementary materials.

Supplementary material

Supplemental data for this article can be accessed online at https://doi.org/10.1080/21505594.2022.2105351

Additional information

Funding

The work was supported by the Ministry of Science and Technology of Taiwan (MOST 111-2320-B-038-063, MOST 111-2320-B-002-066, MOST 111-2320-B-002-076 and MOST 108-2320-B-038-057-MY3)