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Bioscience, Biotechnology, and Biochemistry Volume 81, 2017 - Issue 12
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Biochemistry & Molecular Biology

Generation of thermostable Moloney murine leukemia virus reverse transcriptase variants using site saturation mutagenesis library and cell-free protein expression system

Yuta KatanoDivision of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Kyoto, Japan
,
Tongyang LiDivision of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Kyoto, Japan
,
Misato BabaDivision of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Kyoto, Japan
,
Miyo NakamuraDivision of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Kyoto, Japan
,
Masaaki ItoDepartment of Bioresources Engineering, National Institute of Technology, Okinawa College, Nago, Japan
,
Kenji KojimaDivision of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Kyoto, Japan
,
Teisuke TakitaDivision of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Kyoto, Japan
&
Kiyoshi YasukawaDivision of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Kyoto, JapanCorrespondence[email protected]
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Pages 2339-2345 | Received 19 Aug 2017, Accepted 12 Oct 2017, Published online: 06 Nov 2017

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Kota Nakatani, Yuta Katano, Kenji Kojima, Teisuke Takita, Rie Yatsunami, Satoshi Nakamura & Kiyoshi Yasukawa. (2018) Increase in the thermostability of Bacillus sp. strain TAR-1 xylanase using a site saturation mutagenesis library. Bioscience, Biotechnology, and Biochemistry 82:10, pages 1715-1723.
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Articles from other publishers (12)

Javier Martínez del Río, Nerea López-Carrobles, Jesús I. Mendieta-Moreno, Óscar Herrera-Chacón, Adrián Sánchez-Ibáñez, Jesús Mendieta & Luis Menéndez-Arias. (2023) Charge engineering of the nucleic acid binding cleft of a thermostable HIV-1 reverse transcriptase reveals key interactions and a novel mechanism of RNase H inactivation. Journal of Molecular Biology, pages 168219.
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Julian Grünewald, Bret R. Miller, Regan N. Szalay, Peter K. Cabeceiras, Christopher J. Woodilla, Eliza Jane B. Holtz, Karl Petri & J. Keith Joung. (2022) Engineered CRISPR prime editors with compact, untethered reverse transcriptases. Nature Biotechnology 41:3, pages 337-343.
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Igor P. Oscorbin, Lidiya M. Novikova & Maxim L. Filipenko. (2022) Comparison of Reverse Transcriptase (RT) Activities of Various M-MuLV RTs for RT-LAMP Assays. Biology 11:12, pages 1809.
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Yuan Zong, Yijing Liu, Chenxiao Xue, Boshu Li, Xiangyang Li, Yanpeng Wang, Ji Li, Guanwen Liu, Xingxu Huang, Xiaofeng Cao & Caixia Gao. (2022) An engineered prime editor with enhanced editing efficiency in plants. Nature Biotechnology 40:9, pages 1394-1402.
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Qianru Lin, Huanyu Wang, Yingying Xu, Dongxue Dong, Qingzhen Miao, Jing Lu, Mingsheng Lyu & Shujun Wang. (2022) Study of key amino acid residues of GH66 dextranase for producing high-degree polymerized isomaltooligosaccharides and improving of thermostability. Frontiers in Bioengineering and Biotechnology 10.
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Miao Tian, Shaowei Huang, Zhiyuan Wang, Junying Fu, Pengmei Lv, Changlin Miao, Tao Liu, Lingmei Yang & Wen Luo. (2021) Enhanced activity of Rhizomucor miehei lipase by directed saturation mutation of the propeptide. Enzyme and Microbial Technology 150, pages 109870.
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Yutaro Narukawa, Mako Kandabashi, Tongyang Li, Misato Baba, Haruka Hara, Kenji Kojima, Kei Iida, Takayoshi Hiyama, Sho Yokoe, Tomomi Yamazaki, Teisuke Takita & Kiyoshi Yasukawa. (2021) Improvement of Moloney murine leukemia virus reverse transcriptase thermostability by introducing a disulfide bridge in the ribonuclease H region. Protein Engineering, Design and Selection 34.
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Igor P. Oscorbin & Maxim L. Filipenko. (2021) M-MuLV reverse transcriptase: Selected properties and improved mutants. Computational and Structural Biotechnology Journal 19, pages 6315-6327.
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Igor P. Oscorbin, Pei Fong Wong, Ulyana A. Boyarskikh, Evgeny A. Khrapov & Maksim L. Filipenko. (2020) The attachment of a DNA‐binding Sso7d‐like protein improves processivity and resistance to inhibitors of M‐MuLV reverse transcriptase. FEBS Letters 594:24, pages 4338-4356.
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Kaichi Hayashi, Takeaki Ikeuchi, Ryo Morishita, Jun Qian, Kenji Kojima, Teisuke Takita, Keisuke Tanaka, Shunji Hattori & Kiyoshi Yasukawa. (2020) The roles of histidine and tyrosine residues in the active site of collagenase in Grimontia hollisae. The Journal of Biochemistry 168:4, pages 385-392.
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Teisuke Takita, Kota Nakatani, Yuta Katano, Manami Suzuki, Kenji Kojima, Naoki Saka, Bunzo Mikami, Rie Yatsunami, Satoshi Nakamura & Kiyoshi Yasukawa. (2019) Increase in the thermostability of GH11 xylanase XynJ from Bacillus sp. strain 41M-1 using site saturation mutagenesis. Enzyme and Microbial Technology 130, pages 109363.
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S Palikša, G Alzbutas & R Skirgaila. (2018) Decreased Km to dNTPs is an essential M-MuLV reverse transcriptase adoption required to perform efficient cDNA synthesis in One-Step RT-PCR assay. Protein Engineering, Design and Selection 31:3, pages 79-89.
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