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Original Article

The Level of Induced DNA Double-strand Breakage Correlates with Cell Killing after X-irradiation

Pages 45-54 | Received 28 Jun 1984, Accepted 03 Jan 1985, Published online: 03 Jul 2009

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J. M. Akudugu, T. Theron, A. M. Serafin & L. Böhm. (2004) Influence of DNA double‐strand break rejoining on clonogenic survival and micronucleus yield in human cell lines. International Journal of Radiation Biology 80:2, pages 93-104.
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K. M. PRISE, G. AHNSTRÖM, M. BELLI, J. CARLSSON, D. FRANKENBERG, J. KIEFER, M. LOÖBRICH, B. D. MICHAEL, J. NYGREN, G. SIMONE & B. STENERLÖW. (1998) A review of dsb induction data for varying quality radiations. International Journal of Radiation Biology 74:2, pages 173-184.
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W. SONTAG. (1997) A discrete cell survival model including repair after high dose-rate of ionizing radiation. International Journal of Radiation Biology 71:2, pages 129-144.
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H. C. NEWMAN, K. M. PRISE, M. FOLKARD and B. D. MICHAEL. (1997) DNA double-strand break distributions in X-ray and alpha-particle irradiated V79 cells: evidence for non-random breakage. International Journal of Radiation Biology 71:4, pages 347-363.
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P. DAZA *, H. SCHUssLER, T. J. McMILLAN, S. C. GIROD and P. PFEIFFER. (1997) Radiosensitivity and double-strand break rejoining in tumorigenic and non-tumorigenic human epithelial cell lines. International Journal of Radiation Biology 72:1, pages 91-100.
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D. MURRAY. (1996) ERCC1/ERCC4 5-endonuclease activity as a determinant of hypoxic cell radiosensitivity. International Journal of Radiation Biology 69:3, pages 319-327.
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P. LAMBIN. (1996) Might intrinsic radioresistance of human tumour cells be induced by radiation?. International Journal of Radiation Biology 69:3, pages 279-290.
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S. MATEOS. (1996) Cell-cycle variation in DNA migration in pulsed-field gel electrophoresis. International Journal of Radiation Biology 69:6, pages 687-693.
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T. Orta, J.J. Eady, J.H. Peacock & G.G. Steel. (1995) Glutathione Manipulation and the Radiosensitivity of Human Tumour and Fibroblast Cell Lines. International Journal of Radiation Biology 68:4, pages 413-419.
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R.S.L. Wong, J.R. Dynlacht, B. Cedervall & W.C. Dewey. (1995) Analysis by Pulsed-field Gel Electrophoresis of DNA Double-strand Breaks Induced by Heat and/or X-irradiation in Bulk and Replicating DNA of CHO Cells. International Journal of Radiation Biology 68:2, pages 141-152.
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J. Wang, D.A. Delgado & D.F. Deen. (1995) Use of Pulsed-field Gel Electrophoresis to Investigate Factors Influencing the Measurement of DNA Double-strand Breaks in Human Brain Tumour Specimens. International Journal of Radiation Biology 67:2, pages 153-160.
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S.J. Whitaker, Y.C. Ung & T.J. McMillan. (1995) DNA Double-strand Break Induction and Rejoining as Determinants of Human Tumour Cell Radiosensitivity. A Pulsed-field Gel Electrophoresis Study. International Journal of Radiation Biology 67:1, pages 7-18.
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K.H. Chadwick & H.P. Leenhouts. (1994) On the Linearity of the Dose—effect Relationship of DNA Double Strand Breaks. International Journal of Radiation Biology 66:5, pages 549-552.
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J.M. Coco-Martin, M.F.M.A. Smeets, M. Poggensee, E. Mooren, I. Hofland, M. van den Brug, C. Ottenheim, H. Bartelink & A.C. Begg. (1994) Use of Fluorescence in Situ Hybridization to Measure Chromosome Aberrations as a Predictor of Radiosensitivity in Human Tumour Cells. International Journal of Radiation Biology 66:3, pages 297-307.
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N. Zaffaroni, L. Orlandi, R. Villa, A. Bearzatto, E.K. Rofstad & R. Silvestrini. (1994) DNA Double-strand Break Repair and Radiation Response in Human Tumour Primary Cultures. International Journal of Radiation Biology 66:3, pages 279-285.
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D. Murray, R. Simpson, E. Rosenberg, A. Carraway & R. Britten. (1994) Correlation between γ-ray-induced DNA Double-strand Breakage and Cell Killing after Biologically Relevant Doses: Analysis by Pulsed-field Gel Electrophoresis. International Journal of Radiation Biology 65:4, pages 419-426.
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K.M. Prise. (1994) Use of Radiation Quality as a Probe for DNA Lesion Complexity. International Journal of Radiation Biology 65:1, pages 43-48.
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M. Flentje, B. Asadpour, D. Latz & K.J. Weber. (1993) Sensitivity of Neutral Filter Elution but Not PFGE Can Be Modified by Non-dsb Chromatin Damage. International Journal of Radiation Biology 63:6, pages 715-724.
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M.F.M.A. Smeets, E.H.M. Mooren & A.C. Begg. (1993) Radiation-induced DNA Damage and Repair in Radiosensitive and Radioresistant Human Tumour Cells Measured by Field Inversion Gel Electrophoresis. International Journal of Radiation Biology 63:6, pages 703-713.
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T.J. Jenner, C.M. deLara, P. O'Neill & D.L. Stevens. (1993) Induction and Rejoining of DNA Double-strand Breaks in V79-4 Mammalian Cells Following γ- and α-irradiation. International Journal of Radiation Biology 64:3, pages 265-273.
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C. Baumstark-Khan. (1993) X-ray-induced DNA Double-strand Breaks as Lethal Lesions in Diploid Human Fibroblasts Compared to Chinese Hamster Ovary Cells. International Journal of Radiation Biology 63:3, pages 305-311.
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M.D. Story, K.C. Garrett, P.J. Tofilon & R.E. Meyn. (1993) Influence of Irradiation Conditions on the Measurement of DNA Double-strand Breaks by Pulsed-field Gel Electrophoresis. International Journal of Radiation Biology 63:3, pages 297-304.
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Bhavanath Jha & W. Pohlit. (1992) Effect of 2-deoxy-d-glucose on DNA Double Strand Break Repair, Cell Survival and Energy Metabolism in Euoxic Ehrlich Ascites Tumour Cells. International Journal of Radiation Biology 62:4, pages 409-415.
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J. Nygren & G. Ahnström. (1992) The Filter Does Not Act as a DNA Size Discriminator in the Neutral Filter Elution Technique. International Journal of Radiation Biology 62:4, pages 397-399.
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Peggy L. Olive. (1992) DNA Organization Affects Cellular Radiosensitivity and Detection of Initial DNA Strand Breaks. International Journal of Radiation Biology 62:4, pages 389-396.
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K.M. Prise, S. Davies & B.D. Michael. (1992) A Comparison of the Chemical Repair Rates of Free Radical Precursors of DNA Damage and Cell Killing in Chinese Hamster V79 Cells. International Journal of Radiation Biology 61:6, pages 721-728.
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A. Green, A. Prager, P.M. Stoudt & D. Murray. (1992) Relationships Between DNA Damage and the Survival of Radiosensitive Mutant Chinese Hamster Cell Lines Exposed to γ-radiation. Part 1: Intrinsic Radiosensitivity. International Journal of Radiation Biology 61:4, pages 465-472.
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S.J. Whitaker & T.J. McMillan. (1992) Oxygen Effect for DNA Double-strand Break Induction Determined by Pulsed-field Gel Electrophoresis. International Journal of Radiation Biology 61:1, pages 29-41.
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M.J. Peak, L. Wang, C.K. Hill & J.G. Peak. (1991) Comparison of Repair of DNA Double-strand Breaks Caused by Neutron or Gamma Radiation in Cultured Human Cells. International Journal of Radiation Biology 60:6, pages 891-898.
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J.L. Schwartz, R. Mustafi, M.A. Beckett, E.A. Czyzewski, E. Farhangi, D.J. Grdina, J. Rotmensch & R.R. Weichselbaum. (1991) Radiation-induced DNA Double-strand Break Frequencies in Human Squamous Cell Carcinoma Cell Lines of Different Radiation Sensitivities. International Journal of Radiation Biology 59:6, pages 1341-1352.
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L. Metzger & G. Iliakis. (1991) Kinetics of DNA Double-strand Break Repair Throughout the Cell Cycle as Assayed by Pulsed Field Gel Electrophoresis in CHO Cells. International Journal of Radiation Biology 59:6, pages 1325-1339.
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G.E. Iliakis, L. Metzger, N. Denko & T.D. Stamato. (1991) Detection of DNA Double-strand Breaks in Synchronous Cultures of CHO Cells by Means of Asymmetric Field Inversion Gel Electrophoresis. International Journal of Radiation Biology 59:2, pages 321-341.
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N.J. McNally, M. Hinchliffe & J. Soranson. (1990) The Effect of Post-irradiation Anisotonic Treatment on Cell Survival and Repair of DNA Damage. International Journal of Radiation Biology 57:3, pages 503-512.
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I.R. Radford. (1990) Rapid Communication. International Journal of Radiation Biology 57:3, pages 479-483.
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M. Spotheim-Maurizot, M. Charlier & R. Sabattier. (1990) DNA Radiolysis by Fast Neutrons. International Journal of Radiation Biology 57:2, pages 301-313.
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S. Suzuki, K. Eguchi-Kasai, T. Kosaka, I. Watanabe, H. Ohara & I. Kaneko. (1990) Time-lapse Microscopy and DNA Double-strand Breakage of Chinese Hamster Cells Under Conditions Promoting or Preventing PLD Repair after Irradiation with 60Co γ Rays. International Journal of Radiation Biology 58:5, pages 769-779.
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T.J. McMillan, A.M. Cassoni, S. Edwards, A. Holmes & J.H. Peacock. (1990) The Relationship of DNA Double-strand Break Induction to Radiosensitivity in Human Tumour Cell Lines. International Journal of Radiation Biology 58:3, pages 427-438.
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T. Kosaka, I. Kaneko & F. Koide. (1990) Correlation between Non-repairable DNA Lesions and Fixation of Cell Damage by Hypertonic Solutions in Chinese Hamster Cells. International Journal of Radiation Biology 58:3, pages 417-425.
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K.M. Prise, M. Folkard, S. Davies & B.D. Michael. (1990) The Irradiation of V79 Mammalian Cells by Protons with Energies below 2 MeV. Part II. Measurement of Oxygen Enhancement Ratios and DNA Damage. International Journal of Radiation Biology 58:2, pages 261-277.
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Song-Mao Chiu, L.R. Friedman & N.L. Oleinick. (1990) The Fate of DNA–protein Crosslinks Formed in γ-Irradiated Metaphase Cells. International Journal of Radiation Biology 58:2, pages 235-247.
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D. Murray, A. Prager, S.C. Vanankeren, E.M. Altschuler, M.S. Kerr, N.H.A. Terry & L. Milas. (1990) Comparative Effect of the Thiols Dithiothreitol, Cysteamine and WR-151326 on Survival and on the Induction of DNA Damage in Cultured Chinese Hamster Ovary Cells Exposed to γ-radiation. International Journal of Radiation Biology 58:1, pages 71-91.
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E. Dikomey. (1990) Induction and Repair of DNA Strand Breaks in X-irradiated Proliferating and Quiescent CHO Cells. International Journal of Radiation Biology 57:6, pages 1169-1182.
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J.F. Ward. (1990) The Yield of DNA Double-strand Breaks Produced Intracellularly by Ionizing Radiation: A Review. International Journal of Radiation Biology 57:6, pages 1141-1150.
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K. Hildenbrand & D. Schulte-Frohlinde. (1989) E.s.r. Studies on the Mechanism of Hydroxyl Radical-induced Strand Breakage of Polyuridylic Acid. International Journal of Radiation Biology 55:5, pages 725-738.
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K.M. Prise, S. Davies & B.D. Michael. (1989) Cell Killing and DNA Damage in Chinese Hamster V79 Cells Treated with Hydrogen Peroxide. International Journal of Radiation Biology 55:4, pages 583-592.
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Ryuichi Okayasu & George Iliakis. (1989) Linear DNA Elution Dose Response Curves Obtained in CHO Cells with Non-unwinding Filter Elution after Appropriate Selection of the Lysis Conditions. International Journal of Radiation Biology 55:4, pages 569-581.
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Kevin M. Prise, Susan Davies & Barry D. Michael. (1989) An Improved Method for the Treatment of Data from DNA Strand Break Measurements Using Filter Elution Techniques with an Internal Standard. International Journal of Radiation Biology 55:3, pages 323-330.
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George Iliakis & Ryuichi Okayasu. (1988) The Level of Induced DNA Double-strand Breaks Does Not Correlate with Cell Killing in X-irradiated Mitotic and G1-phase CHO Cells. International Journal of Radiation Biology 53:3, pages 395-404.
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David Murray, Elizabeth M. Altschuler, Micah S. Kerr, Susanna C. Vanankeren, Raymond E. Meyn & Luka Milas. (1988) Radioprotection of Cultured Chinese Hamster Ovary Cells by WR-255591. International Journal of Radiation Biology 54:2, pages 269-283.
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Roger F. Martin, Glenn D'Cunha, Marshall Pardee & Barry J. Allen. (1988) Induction of Double-strand Breaks Following Neutron Capture by DNA-bound 157Gd. International Journal of Radiation Biology 54:2, pages 205-208.
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Ian R. Radford, George S. Hodgson & Jane P. Matthews. (1988) Critical DNA Target Size Model of Ionizing Radiation-induced Mammalian Cell Death. International Journal of Radiation Biology 54:1, pages 63-79.
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Ryuichi Okayasu, Detlef Bloecher & George Iliakis. (1988) Variation through the Cell Cycle in the Dose-response of DNA Neutral Filter Elution in X-irradiated Synchronous CHO-cells. International Journal of Radiation Biology 53:5, pages 729-747.
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George Iliakis. (1988) Radiation-induced Potentially Lethal Damage: DNA Lesions Susceptible to Fixation. International Journal of Radiation Biology 53:4, pages 541-584.
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Ian R. Radford & Sam Broadhurst. (1988) Aphidicolin Synchronization of Mouse L Cells Perturbs the Relationship between Cell Killing and DNA Double-strand Breakage after X-irradiation. International Journal of Radiation Biology 53:2, pages 205-215.
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Kevin M. Prise, Susan Davies & Barry D. Michael. (1987) The Relationship between Radiation-induced DNA Double-strand Breaks and Cell Kill in Hamster V79 Fibroblasts Irradiated with 250 kVp X-rays, 2·3 MeV Neutrons or 238Pu α-particles. International Journal of Radiation Biology and Related Studies in Physics, Chemistry and Medicine 52:6, pages 893-902.
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Ian R. Radford. (1987) Use of ‘Nuclear Monolayers’ to Identify Factors Influencing DNA Double-Strand Breakage by X-rays. International Journal of Radiation Biology and Related Studies in Physics, Chemistry and Medicine 52:6, pages 853-858.
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Ian R. Radford. (1987) Effect of Cell-cycle Position and Dose on the Kinetics of DNA Double-strand Breakage Repair in X-irradiated Chinese Hamster Cells. International Journal of Radiation Biology and Related Studies in Physics, Chemistry and Medicine 52:4, pages 555-563.
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Ian R. Radford & George S. Hodgson. (1987) Effect of Ploidy on the Response of V79 Cells to Ionizing Radiation. International Journal of Radiation Biology and Related Studies in Physics, Chemistry and Medicine 51:5, pages 765-778.
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R.L. Warters, B.W. Lyons & J. Axtell-Bartlett. (1987) Inhibition of Repair of Radiation-induced DNA Damage by Thermal Shock in Chinese Hamster Ovary Cells. International Journal of Radiation Biology and Related Studies in Physics, Chemistry and Medicine 51:3, pages 505-517.
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Richard A. Gibbs, James Camakaris, George S. Hodgson & Roger F. Martin. (1987) Molecular Characterization of 125I Decay and X-ray-induced HPRT Mutants in CHO Cells. International Journal of Radiation Biology and Related Studies in Physics, Chemistry and Medicine 51:2, pages 193-199.
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G.P. van der Schans, O. Vos, W.S.D. Roos-Verheij & P.H.M. Lohman. (1986) The Influence of Oxygen on the Induction of Radiation Damage in DNA in Mammalian Cells after Sensitization by Intracellular Glutathione Depletion. International Journal of Radiation Biology and Related Studies in Physics, Chemistry and Medicine 50:3, pages 453-465.
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Ian R. Radford & Sam Broadhurst. (1986) Enhanced Induction by X-irradiation of DNA Double-strand Breakage in Mitotic as Compared with S-phase V79 Cells. International Journal of Radiation Biology and Related Studies in Physics, Chemistry and Medicine 49:6, pages 909-914.
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Ian R. Radford. (1985) Effect of Radiomodifying Agents on the Ratios of X-ray-induced Lesions in Cellular DNA: Use in Lethal Lesion Determination. International Journal of Radiation Biology and Related Studies in Physics, Chemistry and Medicine 49:4, pages 621-637.
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Ian R. Radford. (1985) Evidence for a General Relationship between the Induced Level of DNA Double-strand Breakage and Cell-killing after X-irradiation of Mammalian Cells. International Journal of Radiation Biology and Related Studies in Physics, Chemistry and Medicine 49:4, pages 611-620.
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Ian R. Radford & George S. Hodgson. (1985) 125I-induced DNA Double Strand Breaks: Use in Calibration of the Neutral Filter Elution Technique and Comparison with X-ray Induced Breaks. International Journal of Radiation Biology and Related Studies in Physics, Chemistry and Medicine 48:4, pages 555-566.
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