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mAbs Volume 9, 2017 - Issue 6
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Characterization of a novel modification of a CHO-produced mAb: Evidence for the presence of tyrosine sulfation

Jia ZhaoProtein Mass Spectrometry, Sterile Product and Analytical Development, Bioprocess Development, Merck Research Laboratories, Kenilworth, NJ, USACorrespondence[email protected]
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Jason SaundersPreclinical Development, New Modalities, Bioprocess Development, Merck Research Laboratories, Kenilworth, NJ, USAORCID Iconhttp://orcid.org/0000-0003-2944-1419
ORCID Icon,
Svetlana Dukleska SchusslerPurification Process Development & In-Process Analytical, Bioprocess Development, Merck Research Laboratories, Kenilworth, NJ, USA
,
Sandra RiosPurification Process Development & In-Process Analytical, Bioprocess Development, Merck Research Laboratories, Kenilworth, NJ, USA
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Francis Kobina InsaidooPurification Process Development & In-Process Analytical, Bioprocess Development, Merck Research Laboratories, Kenilworth, NJ, USA
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Aleksandr L. FridmanProtein Mass Spectrometry, Sterile Product and Analytical Development, Bioprocess Development, Merck Research Laboratories, Kenilworth, NJ, USA
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Huijuan LiProtein Mass Spectrometry, Sterile Product and Analytical Development, Bioprocess Development, Merck Research Laboratories, Kenilworth, NJ, USA
&
Yan-Hui LiuProtein Mass Spectrometry, Sterile Product and Analytical Development, Bioprocess Development, Merck Research Laboratories, Kenilworth, NJ, USA
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Pages 985-995 | Received 11 Jan 2017, Accepted 16 May 2017, Published online: 02 Aug 2017

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Christopher B. Lietz, Ekaterina Deyanova, Younhee Cho, Jon Cordia, Sarah Franc, Sally Kabro, Steven Wang, David Mikolon & Douglas D. Banks. (2023) Identification of tyrosine sulfation in the variable region of a bispecific antibody and its effect on stability and biological activity. mAbs 15:1.
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Shabdita Vatsa. (2022) In silico prediction of post-translational modifications in therapeutic antibodies. mAbs 14:1.
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Oksana Tyshchuk, Christoph Gstöttner, Dennis Funk, Simone Nicolardi, Stefan Frost, Stefan Klostermann, Tim Becker, Elena Jolkver, Felix Schumacher, Claudia Ferrara Koller, Hans Rainer Völger, Manfred Wuhrer, Patrick Bulau & Michael Mølhøj. (2019) Characterization and prediction of positional 4-hydroxyproline and sulfotyrosine, two post-translational modifications that can occur at substantial levels in CHO cells-expressed biotherapeutics. mAbs 11:7, pages 1219-1232.
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Yingda Xu, Dongdong Wang, Bruce Mason, Tony Rossomando, Ning Li, Dingjiang Liu, Jason K Cheung, Wei Xu, Smita Raghava, Amit Katiyar, Christine Nowak, Tao Xiang, Diane D. Dong, Joanne Sun, Alain Beck & Hongcheng Liu. (2019) Structure, heterogeneity and developability assessment of therapeutic antibodies. mAbs 11:2, pages 239-264.
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Samadhi Vitharana, Joshua M. Stillahn, Derrick S. Katayama, Charles S. Henry & Mark Cornell Manning. (2023) Application of Formulation Principles to Stability Issues Encountered During Processing, Manufacturing, and Storage of Drug Substance and Drug Product Protein Therapeutics. Journal of Pharmaceutical Sciences.
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Xiaotian Zhong & Aaron M. D’Antona. (2022) A potential antibody repertoire diversification mechanism through tyrosine sulfation for biotherapeutics engineering and production. Frontiers in Immunology 13.
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Alain Beck, Christine Nowak, Deborah Meshulam, Kristina Reynolds, David Chen, Dennis B. Pacardo, Samantha B. Nicholls, Gregory J. Carven, Zhenyu Gu, Jing Fang, Dongdong Wang, Amit Katiyar, Tao Xiang & Hongcheng Liu. (2022) Risk-Based Control Strategies of Recombinant Monoclonal Antibody Charge Variants. Antibodies 11:4, pages 73.
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Ren Liu, Yixiao Zhang, Amit Kumar, Steven Huhn, Laurie Hullinger & Zhimei Du. (2021) Modulating tyrosine sulfation of recombinant antibodies in CHO cell culture by host selection and sodium chlorate supplementation. Biotechnology Journal 16:9, pages 2100142.
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James R. Arndt, Kelly L. Wormwood Moser, Gregory Van Aken, Rory M. Doyle, Tatjana Talamantes, Daniel DeBord, Laura Maxon, George Stafford, John Fjeldsted, Bryan Miller & Melissa Sherman. (2021) High-Resolution Ion-Mobility-Enabled Peptide Mapping for High-Throughput Critical Quality Attribute Monitoring. Journal of the American Society for Mass Spectrometry 32:8, pages 2019-2032.
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Doreen A. Wüstenhagen, Phil Lukas, Christian Müller, Simone A. Aubele, Jan-Peter Hildebrandt & Stefan Kubick. (2020) Cell-free synthesis of the hirudin variant 1 of the blood-sucking leech Hirudo medicinalis. Scientific Reports 10:1.
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Haitao Jiang, Wei Xu, Ren Liu, Balrina Gupta, Bruce Kilgore, Zhimei Du & Xiaoyu Yang. (2020) Characterization of Bispecific Antibody Production in Cell Cultures by Unique Mixed Mode Size Exclusion Chromatography. Analytical Chemistry 92:13, pages 9312-9321.
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Natalia Gomez, Jonathan Lull, Xiaorui Yang, Yan Wang, Xin Zhang, Agatha Wieczorek, John Harrahy, Mike Pritchard, Diandra Martinez Cano, Michael Shearer & Chetan Goudar. (2020) Improving product quality and productivity of bispecific molecules through the application of continuous perfusion principles. Biotechnology Progress 36:4.
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Anna C. Robotham & John F. Kelly. 2020. Approaches to the Purification, Analysis and Characterization of Antibody-Based Therapeutics. Approaches to the Purification, Analysis and Characterization of Antibody-Based Therapeutics 1 33 .
Thomas Amann, Valerie Schmieder, Helene Faustrup Kildegaard, Nicole Borth & Mikael Rørdam Andersen. (2019) Genetic engineering approaches to improve posttranslational modification of biopharmaceuticals in different production platforms. Biotechnology and Bioengineering 116:10, pages 2778-2796.
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Alain Beck & Hongcheng Liu. (2019) Macro- and Micro-Heterogeneity of Natural and Recombinant IgG Antibodies. Antibodies 8:1, pages 18.
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Cory King, Rekha Patel, Gomathinayagam Ponniah, Christine Nowak, Alyssa Neill, Zhenyu Gu & Hongcheng Liu. (2018) Characterization of recombinant monoclonal antibody variants detected by hydrophobic interaction chromatography and imaged capillary isoelectric focusing electrophoresis. Journal of Chromatography B 1085, pages 96-103.
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