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Original Articles

Isolation of Uracil Auxotrophs of the Fungus Acremonium cellulolyticus and the Development of a Transformation System with the pyrF Gene

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Pages 245-249 | Received 04 Jul 2011, Accepted 27 Oct 2011, Published online: 22 May 2014

Keep up to date with the latest research on this topic with citation updates for this article.

Read on this site (4)

Warasirin Sornlake, Phatcharamon Rattanaphanjak, Verawat Champreda, Lily Eurwilaichitr, Suthathip Kittisenachai, Sittiruk Roytrakul, Tatsuya Fujii & Hiroyuki Inoue. (2017) Characterization of cellulolytic enzyme system of Schizophyllum commune mutant and evaluation of its efficiency on biomass hydrolysis. Bioscience, Biotechnology, and Biochemistry 81:7, pages 1289-1299.
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Naoyuki Okuda, Tatsuya Fujii, Hiroyuki Inoue, Kazuhiko Ishikawa & Tamotsu Hoshino. (2016) Enhancing cellulase production by overexpression of xylanase regulator protein gene, xlnR, in Talaromyces cellulolyticus cellulase hyperproducing mutant strain. Bioscience, Biotechnology, and Biochemistry 80:10, pages 2065-2068.
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Masahiro Watanabe, Erika Yoshida, Hiroaki Fukada, Hiroyuki Inoue, Mitsunori Tokura & Kazuhiko Ishikawa. (2015) Characterization of a feruloyl esterase B from Talaromyces cellulolyticus. Bioscience, Biotechnology, and Biochemistry 79:11, pages 1845-1851.
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Tatsuya Fujii, Hiroyuki Inoue & Kazuhiko Ishikawa. (2014) Characterization of the xylanase regulator protein gene, xlnR, in Talaromyces cellulolyticus (formerly known as Acremonium cellulolyticus). Bioscience, Biotechnology, and Biochemistry 78:9, pages 1564-1567.
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Articles from other publishers (31)

Shuqi Wu, Run Xu, Mengjiao Su, Can Gao, Yang Liu, Yujia Chen, Guangxin Luan, Xu Jia & Rui Wang. (2022) A pyrF -Based Efficient Genetic Manipulation Platform in Acinetobacter baumannii To Explore the Vital DNA Components of Adaptive Immunity for I-F CRISPR-Cas . Microbiology Spectrum 10:5.
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Alexandra P. Orleneva, Vsevolod A. Serebrianyi, Ekaterina A. Kutukova & Tatiana A. Yampolskaya. (2022) Identification of the Talaromyces cellulolyticus Gene Encoding an Extracellular Enzyme with β-galactosidase Activity and Testing it as a Reporter for Gene Expression Assays. Molecular Biotechnology 64:6, pages 637-649.
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Lulu Chang, Hengqian Lu, Haiqin Chen, Xin Tang, Jianxin Zhao, Hao Zhang, Yong Q. Chen & Wei Chen. (2022) Lipid metabolism research in oleaginous fungus Mortierella alpina: Current progress and future prospects. Biotechnology Advances 54, pages 107794.
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Tatsuya Fujii, Hiroyuki Inoue & Akinori Matsushika. (2021) The Transcription Factor Gene tclB2 Regulates Mannanolytic Enzyme Production in the Fungus Talaromyces cellulolyticus. Applied Biochemistry and Biotechnology 193:10, pages 3163-3172.
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Yiying Yang, Qingqing Sun, Yang Liu, Hanzhi Yin, Wenping Yang, Yang Wang, Ying Liu, Yuxian Li, Shen Pang, Wenxi Liu, Qian Zhang, Fang Yuan, Shiwen Qiu, Jiong Li, Xuefeng Wang, Keqiang Fan, Weishan Wang, Zilong Li & Shouliang Yin. (2021) Development of a pyrF-based counterselectable system for targeted gene deletion in Streptomyces rimosus基于 pyrF 作为反选标记构建适用于龟裂链霉菌的基因敲除系统. Journal of Zhejiang University-SCIENCE B 22:5, pages 383-396.
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Keiko Uechi, Masahiro Watanabe, Tatsuya Fujii, Saori Kamachi & Hiroyuki Inoue. (2020) Identification and Biochemical Characterization of Major β-Mannanase in Talaromyces cellulolyticus Mannanolytic System. Applied Biochemistry and Biotechnology 192:2, pages 616-631.
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Yusuke Nakamichi, Tatsuya Fujii, Masahiro Watanabe, Akinori Matsushika & Hiroyuki Inoue. (2020) Crystal structure of GH30-7 endoxylanase C from the filamentous fungus Talaromyces cellulolyticus . Acta Crystallographica Section F Structural Biology Communications 76:8, pages 341-349.
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Tatsuya Fujii & Akinori Matsushika. (2019) The Putative Transcription Factor Gene thaB Regulates Cellulase and Xylanase Production at the Enzymatic and Transcriptional Level in the Fungus Talaromyces cellulolyticus. Applied Biochemistry and Biotechnology 190:4, pages 1360-1370.
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Verawat Champreda, Wuttichai Mhuantong, Hataikarn Lekakarn, Benjarat Bunterngsook, Pattanop Kanokratana, Xin-Qing Zhao, Fei Zhang, Hiroyuki Inoue, Tatsuya Fujii & Lily Eurwilaichitr. (2019) Designing cellulolytic enzyme systems for biorefinery: From nature to application. Journal of Bioscience and Bioengineering 128:6, pages 637-654.
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Yusuke Nakamichi, Tatsuya Fujii, Thierry Fouquet, Akinori Matsushika & Hiroyuki Inoue. (2019) GH30-7 Endoxylanase C from the Filamentous Fungus Talaromyces cellulolyticus . Applied and Environmental Microbiology 85:22.
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Makoto Nakabayashi, Saori Kamachi, Dominggus Malle, Toshiaki Yanamoto, Seiichiro Kishishita, Tatsuya Fujii, Hiroyuki Inoue & Kazuhiko Ishikawa. (2019) Construction of thermostable cellobiohydrolase I from the fungus Talaromyces cellulolyticus by protein engineering. Protein Engineering, Design and Selection 32:1, pages 33-40.
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Yusuke Nakamichi, Thierry Fouquet, Shotaro Ito, Akinori Matsushika & Hiroyuki Inoue. (2019) Mode of Action of GH30-7 Reducing-End Xylose-Releasing Exoxylanase A (Xyn30A) from the Filamentous Fungus Talaromyces cellulolyticus. Applied and Environmental Microbiology 85:13.
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Yusuke Nakamichi, Thierry Fouquet, Shotaro Ito, Masahiro Watanabe, Akinori Matsushika & Hiroyuki Inoue. (2019) Structural and functional characterization of a bifunctional GH30-7 xylanase B from the filamentous fungus Talaromyces cellulolyticus. Journal of Biological Chemistry 294:11, pages 4065-4078.
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Tatsuya Fujii, Hiroyuki Inoue, Shinichi Yano & Shigeki Sawayama. 2018. Fungal Cellulolytic Enzymes. Fungal Cellulolytic Enzymes 135 154 .
Tatsuya Fujii, Hiroyuki Inoue, Kazuhiko Ishikawa & Tamotsu Hoshino. (2017) Deletion Analysis of GH7 Endoglucanase Gene (cel7B) Promoter Region in a Talaromyces cellulolyticus ligD-Disrupted Strain. Applied Biochemistry and Biotechnology 183:4, pages 1516-1525.
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Hiroyuki Inoue, Seiichiro Kishishita, Akio Kumagai, Misumi Kataoka, Tatsuya Fujii & Kazuhiko Ishikawa. (2015) Contribution of a family 1 carbohydrate-binding module in thermostable glycoside hydrolase 10 xylanase from Talaromyces cellulolyticus toward synergistic enzymatic hydrolysis of lignocellulose. Biotechnology for Biofuels 8:1.
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Hiroyuki Inoue, Shinichi Yano & Shigeki Sawayama. (2015) Effect of β-Mannanase and β-Mannosidase Supplementation on the Total Hydrolysis of Softwood Polysaccharides by the Talaromyces cellulolyticus Cellulase System. Applied Biochemistry and Biotechnology 176:6, pages 1673-1686.
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Shinichi YANO. (2015) Enzymatic Saccharification and Fermentation Technology for Ethanol Production from Woody Biomass木質系バイオマスからのエタノール生産のための酵素糖化および発酵技術. Journal of the Japan Petroleum Institute 58:3, pages 128-134.
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Tatsuya Fujii, Hiroyuki Inoue & Kazuhiko Ishikawa. (2015) Decreased Cellulase and Xylanase Production in the Fungus Talaromyces cellulolyticus by Disruption of tacA and tctA Genes, Encoding Putative Zinc Finger Transcriptional Factors. Applied Biochemistry and Biotechnology 175:6, pages 3218-3229.
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Tatsuya Fujii, Hideaki Koike, Shigeki Sawayama, Shinichi Yano & Hiroyuki Inoue. (2015) Draft Genome Sequence of Talaromyces cellulolyticus Strain Y-94, a Source of Lignocellulosic Biomass-Degrading Enzymes. Genome Announcements 3:1.
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Seiichiro Kishishita, Tatsuya Fujii & Kazuhiko Ishikawa. (2014) Heterologous expression of hyperthermophilic cellulases of archaea Pyrococcus sp. by fungus Talaromyces cellulolyticus. Journal of Industrial Microbiology & Biotechnology 42:1, pages 137-141.
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Masahiro Watanabe, Hiroyuki Inoue, Benchaporn Inoue, Miho Yoshimi, Tatsuya Fujii & Kazuhiko Ishikawa. (2014) Xylanase (GH11) from Acremonium cellulolyticus: homologous expression and characterization. AMB Express 4:1.
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Masahiro Watanabe & Kazuhiko Ishikawa. (2014) Crystallization and preliminary X-ray crystallographic analysis of a putative feruloyl esterase from Talaromyces cellulolyticus . Acta Crystallographica Section F Structural Biology Communications 70:12, pages 1664-1667.
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Koutarou Hayata, Seiya Asada, Tatsuya Fujii, Hiroyuki Inoue, Kazuhiko Ishikawa & Shigeki Sawayama. (2014) Gene Targeting by RNAi-Mediated Knockdown of Potent DNA Ligase IV Homologue in the Cellulase-Producing Fungus Talaromyces cellulolyticus. Applied Biochemistry and Biotechnology 174:5, pages 1697-1704.
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Yu Du, Guizhen Xie, Chunfa Yang, Baishan Fang & Hongwen Chen. (2014) Construction of brewing-wine <italic>Aspergillus oryzae pyrG</italic><sup>&minus;</sup> mutant by <italic>pyrG</italic> gene deletion and its application in homology transformation. Acta Biochimica et Biophysica Sinica 46:6, pages 477-483.
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Seiya Asada, Seiya Watanabe, Tatsuya Fujii, Hiroyuki Inoue, Kazuhiko Ishikawa & Shigeki Sawayama. (2014) RNAi Knockdown of Potent Sugar Sensor in Cellulase-Producing Fungus Acremonium cellulolyticus. Applied Biochemistry and Biotechnology 172:6, pages 3009-3015.
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Tatsuya Fujii, Tamotsu Hoshino, Hiroyuki Inoue & Shinichi Yano. (2014) Taxonomic revision of the cellulose-degrading fungus Acremonium cellulolyticus nomen nudum to Talaromyces based on phylogenetic analysis . FEMS Microbiology Letters 351:1, pages 32-41.
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Seiichiro Kishishita, Miho Yoshimi, Tatsuya Fujii, Larry E. TaylorIIII, Stephen R. Decker, Kazuhiko Ishikawa & Hiroyuki Inoue. (2014) Cellulose-inducible xylanase Xyl10A from Acremonium cellulolyticus: Purification, cloning and homologous expression. Protein Expression and Purification 94, pages 40-45.
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Hiroyuki Inoue, Tatsuya Fujii, Miho Yoshimi, Larry E TaylorIIII, Stephen R Decker, Seiichiro Kishishita, Makoto Nakabayashi & Kazuhiko Ishikawa. (2013) Construction of a starch-inducible homologous expression system to produce cellulolytic enzymes from Acremonium cellulolyticus . Journal of Industrial Microbiology and Biotechnology 40:8, pages 823-830.
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Akihiro Hideno, Hiroyuki Inoue, Tatsuya Fujii, Shinichi Yano, Kenichiro Tsukahara, Katsuji Murakami, Harunobu Yunokawa & Shigeki Sawayama. (2013) High-coverage gene expression profiling analysis of the cellulase-producing fungus Acremonium cellulolyticus cultured using different carbon sources. Applied Microbiology and Biotechnology 97:12, pages 5483-5492.
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Tatsuya Fujii, Hiroyuki Inoue & Kazuhiko Ishikawa. (2013) Enhancing cellulase and hemicellulase production by genetic modification of the carbon catabolite repressor gene, creA, in Acremonium cellulolyticus. AMB Express 3:1, pages 73.
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