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“NextGen” Biologics: Bispecific Antibodies and Emerging Clinical Results

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Pages 675-688 | Received 16 Nov 2015, Accepted 27 Jan 2016, Published online: 16 Mar 2016

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Stephanie Kapelski, Erna Cleiren, Ricardo M. Attar, Ulrike Philippar, Julien Häsler & Mark L. Chiu. (2019) Influence of the bispecific antibody IgG subclass on T cell redirection. mAbs 11:6, pages 1012-1024.
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Sergey E Sedykh, Victor V Prinz, Valentina N Buneva & Georgy A Nevinsky. (2018) Bispecific antibodies: design, therapy, perspectives. Drug Design, Development and Therapy 12, pages 195-208.
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Adam Zwolak, Anthony A. Armstrong, Susan H. Tam, Jose R. Pardinas, Dennis R. Goulet, Songmao Zheng, Kerry Brosnan, Eva Emmell, Jeffrey Luo, Gary L. Gilliland & Mark L. Chiu. (2017) Modulation of protein A binding allows single-step purification of mouse bispecific antibodies that retain FcRn binding. mAbs 9:8, pages 1306-1316.
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Vivek Reddy Murthannagari, Ganesh Gonna Nandhi Krishnan, Kavitha Viswabramhana Manu, Chandu Thoti Jayachandraiah, Praharsh Kumar Mandadhi Rajendra & Syed Suhaib Ahmed. (2023) Exploring the Potential Challenges for Developing Generic Orphan Drugs for Rare Diseases: A Survey of US and European Markets. Value in Health Regional Issues 35, pages 87-94.
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Yan Yang, Zheng Yang & Yun Yang. (2021) Potential Role of CD47-Directed Bispecific Antibodies in Cancer Immunotherapy. Frontiers in Immunology 12.
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Reza Moazzami, Hasan Mirzahoseini, Leila Nematollahi, Farzaneh Barkhordari, Mozhgan Raigani, Fatemeh Hajari Taheri, Fereidoun Mahbudi & Fatemeh Davami. (2021) Woodchuck Hepatitis Virus Post-Transcriptional Regulation Element (WPRE) Promotes Anti-CD19 BiTE Expression in Expi293 Cells. Iranian Biomedical Journal 25:4, pages 275-283.
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Mariam Elshiaty, Hannah Schindler & Petros Christopoulos. (2021) Principles and Current Clinical Landscape of Multispecific Antibodies against Cancer. International Journal of Molecular Sciences 22:11, pages 5632.
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Seyed Samad Hosseini, Saeed Khalili, Behzad Baradaran, Negar Bidar, Mohammad-Ali Shahbazi, Jafar Mosafer, Mahmoud Hashemzaei, Ahad Mokhtarzadeh & Michael R. Hamblin. (2021) Bispecific monoclonal antibodies for targeted immunotherapy of solid tumors: Recent advances and clinical trials. International Journal of Biological Macromolecules 167, pages 1030-1047.
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Zhaoqian Su, Bo Wang, Steven C. Almo & Yinghao Wu. (2020) Understanding the Targeting Mechanisms of Multi-Specific Biologics in Immunotherapy with Multiscale Modeling. iScience 23:12, pages 101835.
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Muhammad Kalim, Muhammad Saleem Iqbal Khan & Jinbiao Zhan. (2020) Programmed cell death ligand‐1: A dynamic immune checkpoint in cancer therapy. Chemical Biology & Drug Design 95:6, pages 552-566.
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Erik Tambuyzer, Benjamin Vandendriessche, Christopher P. Austin, Philip J. Brooks, Kristina Larsson, Katherine I. Miller Needleman, James Valentine, Kay Davies, Stephen C. Groft, Robert Preti, Tudor I. Oprea & Marco Prunotto. (2019) Therapies for rare diseases: therapeutic modalities, progress and challenges ahead. Nature Reviews Drug Discovery 19:2, pages 93-111.
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Raoufeh Ahamadi-Fesharaki, Abolfazl Fateh, Farzam Vaziri, Ghasem Solgi, Seyed Davar Siadat, Fereidoun Mahboudi & Fatemeh Rahimi-Jamnani. (2019) Single-Chain Variable Fragment-Based Bispecific Antibodies: Hitting Two Targets with One Sophisticated Arrow. Molecular Therapy - Oncolytics 14, pages 38-56.
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Weilei Hu, Guosheng Wang, Dongsheng Huang, Meihua Sui & Yibing Xu. (2019) Cancer Immunotherapy Based on Natural Killer Cells: Current Progress and New Opportunities. Frontiers in Immunology 10.
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Roman V. Kholodenko, Daniel V. Kalinovsky, Igor I. Doronin, Eugene D. Ponomarev & Irina V. Kholodenko. (2019) Antibody Fragments as Potential Biopharmaceuticals for Cancer Therapy: Success and Limitations. Current Medicinal Chemistry 26:3, pages 396-426.
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Bo An, Ming Zhang, Jie Pu, Shichen Shen, Yang Qu, Yuan-Ju Chen, Shihan Huo, Xue Wang, Joseph Ryan Polli, Joseph P. Balthasar, Denis Herzog, Luca Ferrari, Roland F. Staack, Wolfgang F. Richter, Michael B. Otteneder, Lisa J. Benincosa, Shaolian Zhou, Faye Vazvaei & Jun Qu. (2019) High-Throughput, Sensitive LC-MS Quantification of Biotherapeutics and Biomarkers Using Antibody-Free, Peptide-Level, Multiple-Mechanism Enrichment via Strategic Regulation of pH and Ionic and Solvent Strengths. Analytical Chemistry 91:5, pages 3475-3483.
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Subhabrata Majumder, Wei Wang & Arun Alphonse Ignatius. (2019) Impact of Buffers on Colloidal Property and Aggregation Propensities of a Bispecific Antibody. Journal of Pharmaceutical Sciences 108:3, pages 1139-1147.
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Jyoti Gupta, Mehboob Hoque, Md Fahim Ahmad, Rizwan Hasan Khan & M. Saleemuddin. (2019) Acid pH promotes bispecific antibody formation by the redox procedure. International Journal of Biological Macromolecules 125, pages 469-477.
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N. Dimasi, R. Fleming, H. Wu & C. Gao. (2019) Molecular engineering strategies and methods for the expression and purification of IgG1-based bispecific bivalent antibodies. Methods 154, pages 77-86.
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Ashley R. Sutherland, Md. Kausar Alam & C. Ronald Geyer. (2018) Post‐translational Assembly of Protein Parts into Complex Devices by Using SpyTag/SpyCatcher Protein Ligase. ChemBioChem 20:3, pages 319-328.
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S. E. Sedykh & G. A. Nevinsky. (2019) Producing and prospects for the use of bispecific antibodies for the treatment of cancer. Advances in molecular oncology 5:4, pages 30-40.
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Sahar Awwad & Ukrit Angkawinitwong. (2018) Overview of Antibody Drug Delivery. Pharmaceutics 10:3, pages 83.
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Jun Li, Hui-Chen Hsu, John D. Mountz & John G. Allen. (2018) Unmasking Fucosylation: from Cell Adhesion to Immune System Regulation and Diseases. Cell Chemical Biology 25:5, pages 499-512.
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Petra Verdino, Shane Atwell & Stephen J Demarest. (2018) Emerging trends in bispecific antibody and scaffold protein therapeutics. Current Opinion in Chemical Engineering 19, pages 107-123.
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Mireya Paulina Velasquez, Challice L. Bonifant & Stephen Gottschalk. (2018) Redirecting T cells to hematological malignancies with bispecific antibodies. Blood 131:1, pages 30-38.
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Dennis R. Goulet, Steven J. Orcutt, Adam Zwolak, Theo Rispens, Aran F. Labrijn, Rob N. de Jong, William M. Atkins & Mark L. Chiu. (2018) Kinetic mechanism of controlled Fab-arm exchange for the formation of bispecific immunoglobulin G1 antibodies. Journal of Biological Chemistry 293:2, pages 651-661.
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Alexandra Cutillo, Joseph H. Chewning, Michael G. Hurst & Avi Madan-Swain. 2018. Pediatric Cancer Genetics. Pediatric Cancer Genetics 101 113 .
Adam Zwolak, Catherine N. Leettola, Susan H. Tam, Dennis R. Goulet, Mehabaw G. Derebe, Jose R. Pardinas, Songmao Zheng, Rose Decker, Eva Emmell & Mark L. Chiu. (2017) Rapid Purification of Human Bispecific Antibodies via Selective Modulation of Protein A Binding. Scientific Reports 7:1.
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Yang Chen & Yan Xu. (2017) Pharmacokinetics of Bispecific Antibody. Current Pharmacology Reports 3:3, pages 126-137.
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Dennis R. Goulet, Adam Zwolak, Mark L. Chiu, Abhinav Nath & William M. Atkins. (2017) Diffusion of Soluble Aggregates of THIOMABs and Bispecific Antibodies in Serum. Biochemistry 56:17, pages 2251-2260.
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Simon Krah, Christian Schröter, Carla Eller, Laura Rhiel, Nicolas Rasche, Jan Beck, Carolin Sellmann, Ralf Günther, Lars Toleikis, Björn Hock, Harald Kolmar & Stefan Becker. (2017) Generation of human bispecific common light chain antibodies by combining animal immunization and yeast display. Protein Engineering Design and Selection.
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Jyoti Gupta, Mehboob Hoque, Masihuz Zaman, Rizwan Hasan Khan & M. Saleemuddin. (2016) A detergent-based procedure for the preparation of IgG-like bispecific antibodies in high yield. Scientific Reports 6:1.
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B. Vijayalakshmi Ayyar, Sushrut Arora & Richard O’Kennedy. (2016) Coming-of-Age of Antibodies in Cancer Therapeutics. Trends in Pharmacological Sciences 37:12, pages 1009-1028.
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