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Scandinavian Journal of Clinical and Laboratory Investigation Volume 50, 1990 - Issue 8
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Original Article

Preanalytical handling of stored urine samples, and measurement of β2-microglobulin, orosomucoid, albumin, transferrin and immunoglobulin G in urine by enzyme-linked immunosorbent assays (ELISA)

E. Vittinghus Department of Clinical Chemistry, University Hospital of Aarhus Kommunehospital, Aarhus, Denmark
Pages 843-849 | Received 19 Jan 1990, Accepted 16 May 1990, Published online: 08 Jul 2009

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Runa M. Andreassen, Jens B. Kronborg, Henrik Schirmer, Ellisiv B. Mathiesen, Toralf Melsom, Bjørn O. Eriksen, Trond G. Jenssen & Marit D. Solbu. (2022) Urinary orosomucoid is associated with diastolic dysfunction and carotid arteriopathy in the general population. Cross-sectional data from the Tromsø study. Scandinavian Cardiovascular Journal 56:1, pages 148-156.
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Karl M. Brobak, Runa M. Andreassen, Toralf Melsom, Aud Høieggen, Jon V. Norvik & Marit D. Solbu. (2022) Associations of urinary orosomucoid, N-acetyl-β-D-glucosaminidase, and albumin with blood pressure and hypertension after 7 years. The Tromsø Study. Blood Pressure 31:1, pages 270-283.
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Camilla S. Kronborg, Jim Allen, Erik Vittinghus & Ulla Breth Knudsen. (2007) Pre-symptomatic increase in urine-orosomucoid excretion in pre-eclamptic women. Acta Obstetricia et Gynecologica Scandinavica 86:8, pages 930-937.
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Q. Shi, R. Mirteregger, D. Falkenhagen & Y. T. Yu. (2000) A novel configuration of bioartificial liver support system based on circulating microcarrier culture. Artificial Cells, Blood Substitutes, and Biotechnology 28:4, pages 273-291.
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B Lisowska-Myjak, J Pachecka, P Witak & S Radowicki. (1999) Comparison of urinary excretion of albumin and alpha-1-antitrypsin in patients with arterial hypertension. Scandinavian Journal of Clinical and Laboratory Investigation 59:2, pages 93-97.
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Jan Tencer, Hans Thysell, Karin Andersson & Anders Grubb. (1997) Long-term Stability of Albumin, Protein HC, Immunoglobulin G, κ- and λ-chain-immunoreactivity, Orosomucoid and α1-antitrypsin in Urine Stored at -20°C. Scandinavian Journal of Urology and Nephrology 31:1, pages 67-71.
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J. Tencer, H. Thysell, K. Andersson & A. Grubb. (1994) Stability of albumin, protein HC, immunoglobulin G, K- AND γ-chain immunoreactivity, orosomucoid and a 1-antitrypsin in urine stored at various conditions. Scandinavian Journal of Clinical and Laboratory Investigation 54:3, pages 199-206.
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Articles from other publishers (18)

Péter Kustán, Balázs Szirmay, Tamás Kőszegi, Andrea Ludány, Gábor L. Kovács, Attila Miseta, Diána Mühl, Balázs Németh, István Kiss, Ádám Németh, Sándor Szabados & Zénó Ajtay. (2017) Monitoring urinary orosomucoid in patients undergoing cardiac surgery: A promising novel inflammatory marker. Clinical Biochemistry 50:18, pages 1002-1006.
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Baki Sadi, Chunsheng Li, Raymond Ko, Joseph Daka, Hamdi Yusuf, Heather Wyatt, Joel Surette, Nick Priest & Nobuyuki Hamada. (2016) A study on the effect of the internal exposure to 210Po on the excretion of urinary proteins in rats. Radiation and Environmental Biophysics 55:2, pages 161-169.
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Pasquale L. Fedele, Kay-Weng Choy, James C. G. Doery, George Grigoriadis, Jake Shortt & Zhong X. Lu. (2014) Inter-laboratory discordance of beta-2 microglobulin results: impact on the validity of the international staging system for multiple myeloma. British Journal of Haematology 166:6, pages 951-953.
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Mari Palviainen, Marja Raekallio, Mari Vainionpää, Suvi Kosonen & Outi Vainio. (2012) Proteomic profiling of dog urine after European adder (Vipera berus berus) envenomation by two-dimensional difference gel electrophoresis. Toxicon 60:7, pages 1228-1234.
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CAMILLA KRONBORG, ERIK VITTINGHUS, JIM ALLEN & ULLA BRETH KNUDSEN. (2011) Excretion patterns of large and small proteins in pre‐eclamptic pregnancies. Acta Obstetricia et Gynecologica Scandinavica 90:8, pages 897-902.
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Allena Ji Ji, Zhiping Jiang, Yuliya Livson, Jennifer Ann Davis, Jasper Xuegong Chu & Naidong Weng. (2010) Challenges in urine bioanalytical assays: overcoming nonspecific binding. Bioanalysis 2:9, pages 1573-1586.
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Kasia Kania, Elizabeth A Byrnes, John P Beilby, Steve A R Webb & Kimberley J Strong. (2010) Urinary proteases degrade albumin: implications for measurement of albuminuria in stored samples. Annals of Clinical Biochemistry: International Journal of Laboratory Medicine 47:2, pages 151-157.
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Wenkui Li, Suyi Luo, Harold T. Smith & Francis L.S. Tse. (2010) Quantitative determination of BAF312, a S1P-R modulator, in human urine by LC–MS/MS: Prevention and recovery of lost analyte due to container surface adsorption. Journal of Chromatography B 878:5-6, pages 583-589.
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M. S. Christiansen, E. Hommel, E. Magid & B. Feldt-Rasmussen. (2005) Orosomucoid in urine is a powerful predictor of cardiovascular mortality in normoalbuminuric patients with type 2 diabetes at five years of follow-up. Diabetologia 48:2, pages 386-393.
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Merete S. Christiansen, Søren Blirup-Jensen, Lise Foged, Marianne Larsen & Erik Magid. (2004) A particle-enhanced turbidimetric immunoassay for quantitative determination of orosomucoid in urine: development, validation and reference values. Clinical Chemistry and Laboratory Medicine (CCLM) 42:10.
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Yuichi NAKAJIMA, Fumio HOSHI, Seiichi HIGUCHI & Seiichi KAWAMURA. (2001) Determination of Canine .BETA.2-Microgloblin in Plasma and Urine by Enzyme-linked Immunosorbent Assay.. Journal of Veterinary Medical Science 63:3, pages 343-345.
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C. J. Schultz, R. N. Dalton, C. Turner, H. A. W. Neil, D. B. Dunger & For The Oxford Regional Prospective Study Group. (2001) Freezing method affects the concentration and variability of urine proteins and the interpretation of data on microalbuminuria. Diabetic Medicine 17:1, pages 7-14.
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Claudia E Gerber, Gernot Bruchelt, Bettina Götze-Speer & Christian P Speer. (2000) Detection by ELISA of low transferrin levels in bronchoalveolar secretions of preterm infants. Journal of Immunological Methods 233:1-2, pages 41-45.
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Akinori Sasaki, Shinichi Oikawa & Takayoshi Toyota. (1999) Microalbuminuria is closely related to diabetic macroangiopathy. Diabetes Research and Clinical Practice 44:1, pages 35-40.
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Ina S Klasen, Louis JM Reichert, Corrie M de Kat Angelino & Jack FM Wetzels. (1999) Quantitative Determination of Low and High Molecular Weight Proteins in Human Urine: Influence of Temperature and Storage Time. Clinical Chemistry 45:3, pages 430-432.
Crossref
A Hollander, J Thissen, G Doekes, D Heederik, S Gordon, KM Venables, A Renström, PH Larsson & P Malmberg. (2001) Comparison of methods to assess airborne rat and mouse allergen levels. I. Analysis of air samples. Allergy 54:2, pages 142-149.
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David J. Webb, David J. Newman, Nishi Chaturvedi & John H. Fulle. (1996) The use of the Micral-Test strip to identify the presence of microalbuminuria in people with insulin dependent diabetes mellitus (IDDM) participating in the EUCLID study. Diabetes Research and Clinical Practice 31:1-3, pages 93-102.
Crossref
JP Bonde & E. Vittinghus. (2016) Urinary excretion of proteins among metal welders. Human & Experimental Toxicology 15:1, pages 1-4.
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