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Original Article

Science and Art in Preparing Tissues Embedded in Plastic for Light Microscopy, with Special Reference to Glycol Methacrylate, Glass Knives and Simple Stains

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Pages 71-97 | Published online: 12 Jul 2009

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Read on this site (79)

Augusto B. Reis, Fernando M. Reis, Paulo G. Salles, Fernanda R.C.L. Almeida, Reginaldo Martello, Wiviane A. Assis, Andre L. Caldeira-Brant, Edson S. Tatsuo & Hélio Chiarini-Garcia. (2021) A fertility-oriented method for histological processing of testicular biopsies in men with azoospermia. Systems Biology in Reproductive Medicine 67:4, pages 314-321.
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Rubim M. Almeida Da Silva & JosepA. Rosselló. (1999) ANATOMICAL STUDIES ON THE SCILLA VERNA (HYACINTHACEAE) COMPLEX. Israel Journal of Plant Sciences 47:2, pages 103-110.
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R. Filmon, D. Chappard & M. F. Baslé. (1997) Scanning and Transmission Electron Microscopy of Poly 2-Hydroxyethyl Methacrylate-Based Biomaterials. Journal of Histotechnology 20:4, pages 343-346.
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Peter O. Gerrits & Richard W. Horobin. (1996) Glycol Methacrylate Embedding for Light Microscopy: Basic Principles and Trouble-Shooting. Journal of Histotechnology 19:4, pages 297-311.
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Padmanabhan Rajini, Jerold A. Last, Stephen A. Mccurdy, Robert Lawson, Randal J. Southard, Krishna P. Allamneni & Hanspeter Witschi. (1995) Lung Injury And Fibrogenic Response To Dusts From Citrus And Grape Harvests. Inhalation Toxicology 7:3, pages 363-376.
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Daniel Chappard, Francis Vocanson & Jean-Pierre Montheard. (1993) Polymerization of Methacrylates Used as Histological Embedding Media: Local Variations and Time Course of Hardness of Methylmethacrylate Blocks. Journal of Histotechnology 16:1, pages 65-68.
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S. K. Bulstra, J. Drukker, R. Kuijer, W. A. Buurman & A. J. van der Linden. (1993) Thionin Staining of Paraffin and Plastic Embedded Sections of Cartilage. Biotechnic & Histochemistry 68:1, pages 20-28.
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Daniel Chappard, Christian Alexandre & Jean-Pierre Montheard. (1992) Polymerization of Methacrylates: “Bubble-Hole” Artifact Reconsidered with Bone Morphometry. Journal of Histotechnology 15:1, pages 51-55.
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Jean-Pierre Montheard, Michel Chatzopoulos & Daniel Chappard. (1992) 2-Hydroxyethyl Methacrylate (HEMA): Chemical Properties and Applications in Biomedical Fields. Journal of Macromolecular Science, Part C 32:1, pages 1-34.
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BruceE. Walker. (1992) No effect of adult dietary fat on tumors induced prenatally by diethylstilbestrol. Nutrition and Cancer 17:2, pages 161-169.
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Sandra L. Thamer & Frank C. Mendel. (1991) Glycol Methacrylate Preparation for Evaluation of Intraneural Implants in Frogs. Journal of Histotechnology 14:3, pages 175-177.
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F. Yasuzumi, A. Ishida & N. Okura. (1990) Cleaning Resin Sections with a Glass Knife for Light Microscopy. Stain Technology 65:2, pages 103-104.
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Roger A. Brumback, Daniel L. Feeback, Richard W. Leech, Judy L. Ketring & Julie J. Davis. (1989) Modification of Brain Processing Techniques: Tissue Embedding in Glycol Methacrylate and Stain Modifications. Journal of Histotechnology 12:3, pages 201-207.
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Daniel chappard, Zenagui Benascar, Christian Alexandre & Jean-Perre Montheard. (1989) Practical Investigations of Radical Polymerization of Glycolmethacrylate as an Embedding Medium. Journal of Histotechnology 12:2, pages 89-92.
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Mabel Pierce & Moses Rodriguez. (1989) Erichrome Stain for Myelin on Osmicated Tissue Embedded in Glycol Methacrylate Plastic. Journal of Histotechnology 12:1, pages 35-36.
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K. Gotfredsen, E. Budtz-jörgensen & L. Nimb Jensen. (1989) A Method for Preparing and Staining Histological Sections Containing Titanium Implants for Light Microscopy. Stain Technology 64:3, pages 121-127.
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Neil D. Broom & Heather Silyn-Roberts. (1989) The Three-Dimensional ‘Knit’ of Collagen Fibrils in Articular Cartilage. Connective Tissue Research 23:4, pages 261-277.
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DavidJ. P. Bassett, Elizabeth Bowen‐Kelly, ConnieL. Elbon & SherrieS. Reichenbaugh. (1988) Rat lung recovery from 3 days of continuous exposure to 0.75 ppm ozone. Journal of Toxicology and Environmental Health 25:3, pages 329-347.
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Holly W. Randall, Thomas M. Monticello & Kevin T. Morgan. (1988) Large Area Sectioning for Morphologic Studies of Nonhuman Primate Nasal Cavities. Stain Technology 63:6, pages 355-362.
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Raymond Colello. (1988) Modifications of a Method for Preparing Retinal Wholemounts for Sectioning. Stain Technology 63:3, pages 183-188.
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Daniel Dziedzic & HaroldJ. White. (1987) Response of t‐cell‐deficient mice to ozone exposure. Journal of Toxicology and Environmental Health 21:1-2, pages 57-71.
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Claude Nagamine & AllenW. Knight. (1987) Induction of Female Breeding Characteristics by Ovarian Tissue Implants in Androgenic Gland Ablated Male Freshwater Prawns Macrobrachium rosenbergii (de Man) (Decapoda, Palaemonidae). International Journal of Invertebrate Reproduction and Development 11:2, pages 225-234.
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H. W. M. van Straaten, J. W. M. Hekking & J. Drukker. (1987) A HCL-Toluidine Blue Staining Procedure Specific for Nuclei, Mitotic Figures and Axons in GMA Embedded Embryonic Neural Tissue. Stain Technology 62:5, pages 360-362.
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Edward C. Yeung & Sandra K. Law. (1987) Serial Sectioning Techniques for A Modified LKB Historesin. Stain Technology 62:3, pages 147-153.
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Monique Hott & Pierre J. Marie. (1987) Glycol Methacrylate as An Embedding Medium for Bone. Stain Technology 62:1, pages 51-57.
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Siegfried Fink. (1987) Some New Methods for Affixing Sections to Glass Slides. I. Aqueous Adhesives. Stain Technology 62:1, pages 27-33.
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J. J. Bruhl & A. E. Ashford. (1986) Application of Glycol Methacrylate Embedding to Herbarium Specimens of Fruits. Stain Technology 61:3, pages 163-167.
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Margaret W. Leigh, Todd M. Gambling, Johnny L. Carson, Albert M. Collier, Robert E. Wood & Thomas F. Boat. (1986) Postnatal Development of Tracheal Surface Epithelium and Submucosal Glands in the Ferret. Experimental Lung Research 10:2, pages 153-169.
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Marlene D. Castro. (1985) A Hematoxylin-Eosin Phloxine Stain for Tissues Embedded in Glycol Methacrylate. Journal of Histotechnology 8:1, pages 23-24.
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Helen E. Gruber, G. June Marshall, Mary E. Kirchen, Jung Kang & Shaul G. Massry. (1985) Improvements in Dehydration and Cement Line Staining for Methacrylate Embedded Human Bone Biopsies. Stain Technology 60:6, pages 337-344.
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Edward V. Caruolo & Patricia Minchew. (1985) A Method for Silver Impregnation of Mammary Myoepithelia. Stain Technology 60:5, pages 255-260.
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R. Frater. (1985) A Removable Polar Embedding Medium for Light Microscopy. Stain Technology 60:4, pages 189-192.
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Judith McNiff & Joan Gil. (1985) Notes on Technic. Stain Technology 60:3, pages 181-187.
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J. L. Myhre & A. Depaoli. (1985) A Glycol Methacrylate Method for the Routine Histologic Evaluation of Rat Inner Ear. Stain Technology 60:2, pages 63-68.
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John W. Taylor. (1984) Correlative Light and Electron Microscopy with Fluorescent Stains. Mycologia 76:3, pages 462-467.
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Jacques Bernier, Pierre R. Filion & Michel G. Cote. (1984) Improved Stability of a Purified Glycol Methacrylate Preparation. Stain Technology 59:6, pages 355-355.
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P. Anthony di Sant'Agnese & Karen L. De Mesy-Jensen. (1984) Diagnostic Electron Microscopy on Reembedded (“Popped Off”) Areas of Large Spun* Epoxy Sections. Ultrastructural Pathology 6:2-3, pages 247-252.
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Jei W. Ryoo & Robert J. Buschmann. (1983) Caveat on Plastic Embedding of Perfused Normal Liver for Histology. Journal of Histotechnology 6:3, pages 138-140.
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Richard M. Franklin & Maria-Teresa Martin. (1983) An Improved Formulation of Nocht's Azure-Eosin Stain for Plastic Embedded Material. Stain Technology 58:6, pages 353-356.
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Daniel Chappard, Christian Alexandre, Marcel Camps, Jean Pierre Montheard & Georges Riffat. (1983) Embedding Iliac Bone Biopsies at Low Temperature using Glycol and Methyl Methacrylates. Stain Technology 58:5, pages 299-308.
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Robert F. Vogt, Noreen A. Hynes, Arthur M. Dannenberg, Steven Castracane & Louis Weiss. (1983) Improved Techniques Using Giemsa Stained Glycol Methacrylate Tissue Sections to Quantitate Basophils and Other Leukocytes in Inflammatory Skin Lesions. Stain Technology 58:4, pages 193-205.
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Paul J. McMillan, Paul C. Engen, Arthur Dalgleish & James McMillan. (1983) Improvement of the Butyl Methacrylate-Paraffin Embedment. Stain Technology 58:3, pages 125-130.
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D. Grenville. (1983) A New Method for Flat Embedding in Glycol Methacrylate. Stain Technology 58:1, pages 57-58.
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Garry T. Cole. (1983) Graphiola Phoenicis: A Taxonomic Enigma. Mycologia 75:1, pages 93-116.
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G. H. Kenner, L. Henricks, G. Gimenez, W. Barb & J. B. Park. (1982) Bone Embedding Technique with Inhibited Pmma Monomer. Stain Technology 57:2, pages 121-126.
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Effin T. Graham. (1982) Improved Diethylene Glycol Distearate Embedding Wax. Stain Technology 57:1, pages 39-43.
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Michael D. McGinn, Richard A. Chole & Kenneth R. Henry. (1982) Cholesteatoma: Experimental Induction in the Mongolian Gerbil, Meriones Unguiculaus. Acta Oto-Laryngologica 93:1-6, pages 61-67.
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R. M. Franklin, M. T. Martin & R. Longato. (1981) A Simple Procedure for Extracting Methacrylic Acid from Water-Miscible Methacrylates. Stain Technology 56:5, pages 283-285.
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Henry Troyer & Elaine Babich. (1981) A Hematoxylin and Eosin-Like Stain for Glycol Methacrylate Embedded Tissue Sections. Stain Technology 56:1, pages 39-43.
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William A. Horton, Nena Dockery, David Sillence & David L. Rimoin. (1980) An Embedding Method for Histochemical Studies of Undecalcified Skeletal Growth Plate. Stain Technology 55:1, pages 19-29.
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James K. Butler. (1980) Note on Technic: The Use of Ralph-Bennett Glass Knives for Specimen Block Trimming. Stain Technology 55:5, pages 323-324.
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Richard M. Franklin & Maria-Teresa Martin. (1980) Staining and Histochemistry of Undecalcified Bone Embedded in a Water-Miscible Plastic. Stain Technology 55:5, pages 313-321.
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Jane M. Woodruff & Stanley A. Greenfield. (1979) Advantages of Glycol Methacrylate Embedding Systems for Light Microscopy. Journal of Histotechnology 2:4, pages 164-167.
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Clyde N. Lulham. (1979) Glycol Methacrylate Embedding or Light Microscopy. Journal of Histotechnology 2:2, pages 68-71.
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James K. Butler. (1979) Methods for Improved Light Microscope Microtomy. Stain Technology 54:2, pages 53-69.
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. (1979) Notes on Technic. Stain Technology 54:5, pages 281-295.
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R. Semba. (1979) Contributions to Semithin Sextioning on A Conventional Rotary Microtome. Stain Technology 54:5, pages 251-255.
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Charles Pandolph & L. W. Poulter. (1978) The Staining Of Acid Fast Bacilli In Sections Of Glycol Methacrylate Embedded Tissues. Stain Technology 53:3, pages 173-176.
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Kevin Grayson. (1978) Notes on Technic. Stain Technology 53:2, pages 115-120.
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Katsuji Kaneko & Masumi Akita. (1978) Notes on Technic. Stain Technology 53:1, pages 43-59.
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M. R. Zingeser & W. P. McNulty. (1977) A Method of Relating Craniofacial Sections to Topography in Embryos. Stain Technology 52:6, pages 327-330.
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