4,913
Views
61
CrossRef citations to date
0
Altmetric
Research Article

A framework for in vitro systems toxicology assessment of e-liquids

, , , , , , , , , , , , , , & show all
Pages 392-416 | Received 16 Feb 2016, Accepted 21 Mar 2016, Published online: 26 Apr 2016

Keep up to date with the latest research on this topic with citation updates for this article.

Read on this site (4)

Riccardo Polosa, Renée O’Leary, Donald Tashkin, Rosalia Emma & Massimo Caruso. (2019) The effect of e-cigarette aerosol emissions on respiratory health: a narrative review. Expert Review of Respiratory Medicine 13:9, pages 899-915.
Read now
Guanghe Wang, Wenjing Liu & Weimin Song. (2019) Toxicity assessment of electronic cigarettes. Inhalation Toxicology 31:7, pages 259-273.
Read now
Tomasz R. Sosnowski, Katarzyna Jabłczyńska, Marcin Odziomek, Walter K. Schlage & Arkadiusz K. Kuczaj. (2018) Physicochemical studies of direct interactions between lung surfactant and components of electronic cigarettes liquid mixtures. Inhalation Toxicology 30:4-5, pages 159-168.
Read now
Rakesh Dixit. (2016) Special issue on electronic cigarettes. Toxicology Mechanisms and Methods 26:6, pages 389-391.
Read now

Articles from other publishers (57)

Aijing Xu, Kun Duan, Wanchun Yang, Guodong Feng, Zehong Wu, Xingtao Jiang, Min Li, Peiqing Liu & Jianwen Chen. (2023) The toxic effects of electronic cigarette aerosol and cigarette smoke on cardiovascular, gastrointestinal and renal systems in mice. Scientific Reports 13:1.
Crossref
E. Bishop, N. East, F. Miazzi, S. Fiebelkorn, D. Breheny, M. Gaca & D. Thorne. (2023) A contextualised e-cigarette testing strategy shows flavourings do not impact lung toxicity in vitro. Toxicology Letters 380, pages 1-11.
Crossref
Stephanie Johne, Marco van der Toorn, Anita R. Iskandar, Shoaib Majeed, Laura O. Torres, Julia Hoeng & Manuel C. Peitsch. (2023) An in vitro evaluation of e-vapor products: The contributions of chemical adulteration, concentration, and device power. Food and Chemical Toxicology 175, pages 113708.
Crossref
Amelia-Naomi Sabo, Emma Filaudeau, Sylvia Da Silva, Guillaume Becker, Laurent Monassier & Véronique Kemmel. (2023) Flavoured and nicotine-containing e-liquids impair homeostatic properties of an alveolar-capillary cell model. Food and Chemical Toxicology 174, pages 113650.
Crossref
Nargiz Travis, Marie Knoll, Steven Cook, Hayoung Oh, Christopher J. Cadham, Luz María Sánchez-Romero & David T. Levy. (2023) Chemical Profiles and Toxicity of Electronic Cigarettes: An Umbrella Review and Methodological Considerations. International Journal of Environmental Research and Public Health 20:3, pages 1908.
Crossref
Hongjuan Wang, Shulei Han, Huan Chen, Peizhen Li, Shigang Li, Yujuan Wu, Chunxia Zhang, Yaning Fu, Yushan Tian, Tong Liu, Hongwei Hou & Qingyuan Hu. (2022) In Vitro Toxicological Investigation and Risk Assessment of E-Cigarette Aerosols Based on a Novel Solvent-Free Extraction Method. ACS Omega 7:51, pages 48403-48415.
Crossref
Valérie Forest, Clément Mercier & Jérémie Pourchez. (2022) Considerations on dosimetry for in vitro assessment of e-cigarette toxicity. Respiratory Research 23:1.
Crossref
J. Zhang, U. Doshi, R.L. Wolz, P. Kosachevsky, M.J. Oldham, I.G. Gillman & K.M. Lee. (2022) Fit-for-purpose characterization of air-liquid-interface (ALI) in vitro exposure systems for e-vapor aerosol. Toxicology in Vitro 82, pages 105352.
Crossref
K.-C. Lin, C.-Z. Yen, J.-W. Yang, J.H.Y. Chung & G.-Y. Chen. (2022) Airborne toxicological assessment: The potential of lung-on-a-chip as an alternative to animal testing. Materials Today Advances 14, pages 100216.
Crossref
Lilan Wang, Yao Wang, Jianwen Chen, Peiqing Liu & Min Li. (2022) A Review of Toxicity Mechanism Studies of Electronic Cigarettes on Respiratory System. International Journal of Molecular Sciences 23:9, pages 5030.
Crossref
Massimo Caruso, Rosalia Emma, Sonja Rust, Alfio Distefano, Giuseppe Carota, Roberta Pulvirenti, Riccardo Polosa & Giovanni Li Volti. (2021) Screening of different cytotoxicity methods for the assessment of ENDS toxicity relative to tobacco cigarettes. Regulatory Toxicology and Pharmacology 125, pages 105018.
Crossref
Hae-Ryung Park, Jose Vallarino, Michael O’Sullivan, Charlotte Wirth, Ronald A. Panganiban, Gabrielle Webb, Maya Shumyatcher, Blanca E. Himes, Jin-Ah Park, David C. Christiani, Joseph Allen & Quan Lu. (2021) Electronic cigarette smoke reduces ribosomal protein gene expression to impair protein synthesis in primary human airway epithelial cells. Scientific Reports 11:1.
Crossref
Tanya J. Bennet, Avineet Randhawa, Jessica Hua & Karen C. Cheung. (2021) Airway-On-A-Chip: Designs and Applications for Lung Repair and Disease. Cells 10:7, pages 1602.
Crossref
Yuna Cao, Daming Wu, Ying Ma, Xinmo Ma, Shile Wang, Fuxian Li, Menghan Li & Ting Zhang. (2021) Toxicity of electronic cigarettes: A general review of the origins, health hazards, and toxicity mechanisms. Science of The Total Environment 772, pages 145475.
Crossref
Lukasz Czekala, Fiona Chapman, Liam Simms, Kathryn Rudd, Edgar Trelles Sticken, Roman Wieczorek, Lisa Maria Bode, Jutta Pani, Nynke Moelijker, Remco Derr, Inger Brandsma, Giel Hendriks, Matthew Stevenson & Tanvir Walele. (2021) The in vitro ToxTracker and Aneugen Clastogen Evaluation extension assay as a tool in the assessment of relative genotoxic potential of e-liquids and their aerosols . Mutagenesis 36:2, pages 129-142.
Crossref
Oscar M. Camacho, James K. Ebajemito, Steven Coburn, Krishna Prasad, Sandra Costigan & James J. Murphy. (2021) Evidence From the Scientific Assessment of Electronic Cigarettes and Their Role in Tobacco Harm Reduction. Contributions to Tobacco & Nicotine Research 30:2, pages 63-108.
Crossref
David Thorne, Jason Adamson, Edgar Trelles Sticken, Roman Wieczorek, Holger Behrsing, Sandro Steiner, Shoaib Majeed, Stefan Frentzel, Shinkichi Ishikawa, Shigeaki Ito, Liam Simms, Kei Yoshino, Julia Hoeng & Marianna Gaca. (2021) An interlaboratory in vitro aerosol exposure system reference study. Toxicology Research and Application 5, pages 239784732199275.
Crossref
A Beklen & D Uckan. (2020) Electronic cigarette liquid substances propylene glycol and vegetable glycerin induce an inflammatory response in gingival epithelial cells. Human & Experimental Toxicology 40:1, pages 25-34.
Crossref
Lukasz Czekala, Roman Wieczorek, Liam Simms, Fan Yu, Jessica Budde, Edgar Trelles Sticken, Kathryn Rudd, Thomas Verron, Oleg Brinster, Matthew Stevenson & Tanvir Walele. (2021) Multi-endpoint analysis of human 3D airway epithelium following repeated exposure to whole electronic vapor product aerosol or cigarette smoke. Current Research in Toxicology 2, pages 99-115.
Crossref
Diego Marescotti, Carole Mathis, Anne May, Davide Sciuscio, Manuel C. Peitsch & Julia Hoeng. 2021. Toxicological Evaluation of Electronic Nicotine Delivery Products. Toxicological Evaluation of Electronic Nicotine Delivery Products 367 383 .
Carine Poussin, Anita R. Iskandar, Carole Mathis, Daniel J. Smart, Filippo Zanetti, Marco van der Toorn, David Bovard, Rebecca Savioz, Damian McHugh, Walter K. Schlage, Manuel C. Peitsch, Patrick Vanscheeuwijck & Julia Hoeng. 2021. Toxicological Evaluation of Electronic Nicotine Delivery Products. Toxicological Evaluation of Electronic Nicotine Delivery Products 257 304 .
Julia Hoeng, Marja Talikka, Bjoern Titz, Amin Choukrallah, Stéphanie Boué, Nikolai V. Ivanov, Diego Marescotti, Florian Martin & Manuel C. Peitsch. 2021. Toxicological Evaluation of Electronic Nicotine Delivery Products. Toxicological Evaluation of Electronic Nicotine Delivery Products 189 206 .
Julia Hoeng, Stéphanie Boué & Manuel C. Peitsch. 2021. Toxicological Evaluation of Electronic Nicotine Delivery Products. Toxicological Evaluation of Electronic Nicotine Delivery Products 23 40 .
Eva Correia-Álvarez, James E Keating, Gary Glish, Robert Tarran & M Flori Sassano. (2020) Reactive Oxygen Species, Mitochondrial Membrane Potential, and Cellular Membrane Potential Are Predictors of E-Liquid Induced Cellular Toxicity. Nicotine & Tobacco Research 22:Supplement_1, pages S4-S13.
Crossref
Jenny Ho, Davide Sciuscio, Ulrike Kogel, Bjoern Titz, Patrice Leroy, Gregory Vuillaume, Marja Talikka, Elyette Martin, Pavel Pospisil, Stefan Lebrun, Wenhao Xia, Tom Lee, Yun Xuan Chng, Blaine W. Phillips, Emilija Veljkovic, Emmanuel Guedj, Yang Xiang, Nikolai V. Ivanov, Manuel C. Peitsch, Julia Hoeng & Patrick Vanscheeuwijck. (2020) Evaluation of toxicity of aerosols from flavored e-liquids in Sprague–Dawley rats in a 90-day OECD inhalation study, complemented by transcriptomics analysis. Archives of Toxicology 94:6, pages 2179-2206.
Crossref
Carla Frege, Mahdi Asgari, Sandro Steiner, Sandra Ferreira, Shoaib Majeed, Francesco Lucci, Stefan Frentzel, Julia Hoeng & Arkadiusz K. Kuczaj. (2020) Assessment of Single-Photon Ionization Mass Spectrometry for Online Monitoring of in Vitro Aerosol Exposure Experiments . Chemical Research in Toxicology 33:2, pages 505-514.
Crossref
Martin Chaumont, Vanessa Tagliatti, El Mehdi Channan, Jean-Marie Colet, Alfred Bernard, Sofia Morra, Guillaume Deprez, Alain Van Muylem, Nadia Debbas, Thomas Schaefer, Vitalie Faoro & Philippe van de Borne. (2020) Short halt in vaping modifies cardiorespiratory parameters and urine metabolome: a randomized trial. American Journal of Physiology-Lung Cellular and Molecular Physiology 318:2, pages L331-L344.
Crossref
Stéphanie Boué, Didier Goedertier, Julia Hoeng, Anita Iskandar, Arkadiusz K Kuczaj, Diego Marescotti, Carole Mathis, Anne May, Blaine Phillips, Manuel C Peitsch, Walter K Schlage, Davide Sciuscio, Wei Teck Tan & Patrick Vanscheeuwijck. (2020) State-of-the-art methods and devices for generation, exposure, and collection of aerosols from e-vapor products. Toxicology Research and Application 4, pages 239784732097975.
Crossref
Stéphanie Boué, Didier Goedertier, Julia Hoeng, Arkadiusz Kuczaj, Shoaib Majeed, Carole Mathis, Anne May, Blaine Phillips, Manuel C Peitsch, Falk Radtke, Walter K Schlage, Wei Teck Tan & Patrick Vanscheeuwijck. (2020) State-of-the-art methods and devices for the generation, exposure, and collection of aerosols from heat-not-burn tobacco products. Toxicology Research and Application 4, pages 239784731989786.
Crossref
Efthalia Kerasioti, Aristidis S. Veskoukis, Zoi Skaperda, Apostolis Zacharias, Konstantinos Poulas, George Lazopoulos & Demetrios Kouretas. (2020) The flavoring and not the nicotine content is a decisive factor for the effects of refill liquids of electronic cigarette on the redox status of endothelial cells. Toxicology Reports 7, pages 1095-1102.
Crossref
David Thorne, James Whitwell, Julie Clements, Paul Walker, Damien Breheny & Marianna Gaca. (2020) The genotoxicological assessment of a tobacco heating product relative to cigarette smoke using the in vitro micronucleus assay. Toxicology Reports 7, pages 1010-1019.
Crossref
Diego Marescotti, Carole Mathis, Vincenzo Belcastro, Patrice Leroy, Stefano Acali, Florian Martin, Rémi Dulize, David Bornand, Dariusz Peric, Emmanuel Guedj, Laura Ortega Torres, Matteo Biasioli, Matthieu Fuhrimann, Estela Fernandes, Felix Frauendorfer, Ignacio Gonzalez Suarez, Davide Sciuscio, Nikolai V. Ivanov, Manuel C. Peitsch & Julia Hoeng. (2020) Systems toxicology assessment of a representative e-liquid formulation using human primary bronchial epithelial cells. Toxicology Reports 7, pages 67-80.
Crossref
Florian Martin, Sylvain Gubian, Marja Talikka, Julia Hoeng & Manuel C. Peitsch. (2019) NPA: an R package for computing network perturbation amplitudes using gene expression data and two-layer networks. BMC Bioinformatics 20:1.
Crossref
Anita R. Iskandar, Filippo Zanetti, Diego Marescotti, Bjorn Titz, Alain Sewer, Athanasios Kondylis, Patrice Leroy, Vincenzo Belcastro, Laura Ortega Torres, Stefano Acali, Shoaib Majeed, Sandro Steiner, Keyur Trivedi, Emmanuel Guedj, Celine Merg, Thomas Schneider, Stefan Frentzel, Florian Martin, Nikolai V. Ivanov, Manuel C. Peitsch & Julia Hoeng. (2019) Application of a multi-layer systems toxicology framework for in vitro assessment of the biological effects of Classic Tobacco e-liquid and its corresponding aerosol using an e-cigarette device with MESH™ technology. Archives of Toxicology 93:11, pages 3229-3247.
Crossref
Shigeaki Ito, Mark Taylor, Sakura Mori, David Thorne, Tomoki Nishino, Damien Breheny, Marianna Gaça, Kei Yoshino & Christopher Proctor. (2019) An inter-laboratory in vitro assessment of cigarettes and next generation nicotine delivery products. Toxicology Letters 315, pages 14-22.
Crossref
Lindsay K. Caesar, Sabina Nogo, Cassandra N. Naphen & Nadja B. Cech. (2019) Simplify: A Mass Spectrometry Metabolomics Approach to Identify Additives and Synergists from Complex Mixtures. Analytical Chemistry 91:17, pages 11297-11305.
Crossref
Anita R. Iskandar, Filippo Zanetti, Athanasios Kondylis, Florian Martin, Patrice Leroy, Shoaib Majeed, Sandro Steiner, Yang Xiang, Laura Ortega Torres, Keyur Trivedi, Emmanuel Guedj, Celine Merg, Stefan Frentzel, Nikolai V. Ivanov, Utkarsh Doshi, Kyeonghee Monica Lee, Willie J. McKinneyJrJr, Manuel C. Peitsch & Julia Hoeng. (2019) A lower impact of an acute exposure to electronic cigarette aerosols than to cigarette smoke in human organotypic buccal and small airway cultures was demonstrated using systems toxicology assessment. Internal and Emergency Medicine 14:6, pages 863-883.
Crossref
Lukasz Czekala, Liam Simms, Matthew Stevenson, Edgar Trelles-Sticken, Paul Walker & Tanvir Walele. (2019) High Content Screening in NHBE cells shows significantly reduced biological activity of flavoured e-liquids, when compared to cigarette smoke condensate. Toxicology in Vitro 58, pages 86-96.
Crossref
Martin Chaumont, Philippe van de Borne, Alfred Bernard, Alain Van Muylem, Guillaume Deprez, Julien Ullmo, Eliza Starczewska, Rachid Briki, Quentin de Hemptinne, Wael Zaher & Nadia Debbas. (2019) Fourth generation e-cigarette vaping induces transient lung inflammation and gas exchange disturbances: results from two randomized clinical trials. American Journal of Physiology-Lung Cellular and Molecular Physiology 316:5, pages L705-L719.
Crossref
E. Bishop, L. Haswell, J. Adamson, S. Costigan, D. Thorne & M. Gaca. (2019) An approach to testing undiluted e-cigarette aerosol in vitro using 3D reconstituted human airway epithelium. Toxicology in Vitro 54, pages 391-401.
Crossref
A Wallace Hayes, Roman Li, Julia Hoeng, Anita Iskandar, Manuel C Peistch & Michael L Dourson. (2019) New approaches to risk assessment of chemical mixtures. Toxicology Research and Application 3, pages 239784731882076.
Crossref
Shama Ahmad, Iram Zafar, Nithya Mariappan, Maroof Husain, Chih-Chang Wei, Nilam Vetal, Isam A. Eltoum & Aftab Ahmad. (2019) Acute pulmonary effects of aerosolized nicotine. American Journal of Physiology-Lung Cellular and Molecular Physiology 316:1, pages L94-L104.
Crossref
Robert D. Combes & Michael Balls. 2019. The History of Alternative Test Methods in Toxicology. The History of Alternative Test Methods in Toxicology 197 208 .
Alain Sewer, Marja Talikka, Florian Martin, Julia Hoeng & Manuel C Peitsch. 2018. Bioinformatics in the Era of Post Genomics and Big Data. Bioinformatics in the Era of Post Genomics and Big Data.
Laura E. Crotty Alexander, Christopher A. Drummond, Mark Hepokoski, Denzil Mathew, Alex Moshensky, Andrew Willeford, Soumita Das, Prabhleen Singh, Zach Yong, Jasmine H. Lee, Kevin Vega, Ashley Du, John Shin, Christian Javier, Jiang Tian, Joan Heller Brown & Ellen C. Breen. (2018) Chronic inhalation of e-cigarette vapor containing nicotine disrupts airway barrier function and induces systemic inflammation and multiorgan fibrosis in mice. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 314:6, pages R834-R847.
Crossref
D. Thorne, M. Hollings, A. Seymour, J. Adamson, A. Dalrymple, M. Ballantyne & M. Gaca. (2018) Extreme testing of undiluted e-cigarette aerosol in vitro using an Ames air-agar-interface technique. Mutation Research/Genetic Toxicology and Environmental Mutagenesis 828, pages 46-54.
Crossref
M. Flori Sassano, Eric S. Davis, James E. Keating, Bryan T. Zorn, Tavleen K. Kochar, Matthew C. Wolfgang, Gary L. Glish & Robert Tarran. (2018) Evaluation of e-liquid toxicity using an open-source high-throughput screening assay. PLOS Biology 16:3, pages e2003904.
Crossref
Mark Taylor, David Thorne, Tony Carr, Damien Breheny, Paul Walker, Christopher Proctor & Marianna Gaça. (2018) Assessment of novel tobacco heating product THP1.0. Part 6: A comparative in vitro study using contemporary screening approaches. Regulatory Toxicology and Pharmacology 93, pages 62-70.
Crossref
Sébastien Anthérieu, Anne Garat, Nicolas Beauval, Mélissa Soyez, Delphine Allorge, Guillaume Garçon & Jean-Marc Lo-Guidice. (2017) Comparison of cellular and transcriptomic effects between electronic cigarette vapor and cigarette smoke in human bronchial epithelial cells. Toxicology in Vitro 45, pages 417-425.
Crossref
James Murphy, Marianna Gaca, Frazer Lowe, Emmanuel Minet, Damien Breheny, Krishna Prasad, Oscar Camacho, Ian M. Fearon, Chuan Liu, Christopher Wright, Kevin McAdam & Christopher Proctor. (2017) Assessing modified risk tobacco and nicotine products: Description of the scientific framework and assessment of a closed modular electronic cigarette. Regulatory Toxicology and Pharmacology 90, pages 342-357.
Crossref
Blaine Phillips, Bjoern Titz, Ulrike Kogel, Danilal Sharma, Patrice Leroy, Yang Xiang, Grégory Vuillaume, Stefan Lebrun, Davide Sciuscio, Jenny Ho, Catherine Nury, Emmanuel Guedj, Ashraf Elamin, Marco Esposito, Subash Krishnan, Walter K. Schlage, Emilija Veljkovic, Nikolai V. Ivanov, Florian Martin, Manuel C. Peitsch, Julia Hoeng & Patrick Vanscheeuwijck. (2017) Toxicity of the main electronic cigarette components, propylene glycol, glycerin, and nicotine, in Sprague-Dawley rats in a 90-day OECD inhalation study complemented by molecular endpoints. Food and Chemical Toxicology 109, pages 315-332.
Crossref
Alyssa Zucchet & Grégory Schmaltz. (2017) Electronic cigarettes—A review of the physiological health effects. FACETS 2:1, pages 575-609.
Crossref
Ignacio Gonzalez-SuarezDiego MarescottiFlorian MartinElena ScottiEmmanuel GuedjStefano AcaliRemi DulizeKarine BaumerDariusz PericStefan FrentzelNikolai V. IvanovJulia HoengManuel C. Peitsch. (2017) In Vitro Systems Toxicology Assessment of Nonflavored e-Cigarette Liquids in Primary Lung Epithelial Cells . Applied In Vitro Toxicology 3:1, pages 41-55.
Crossref
Wanda FieldsAnna MaioneBrian KeyserBetsy Bombick. (2017) Characterization and Application of the VITROCELL VC1 Smoke Exposure System and 3D EpiAirway Models for Toxicological and e-Cigarette Evaluations. Applied In Vitro Toxicology 3:1, pages 68-83.
Crossref
Allison M. Glasser, Lauren Collins, Jennifer L. Pearson, Haneen Abudayyeh, Raymond S. Niaura, David B. Abrams & Andrea C. Villanti. (2017) Overview of Electronic Nicotine Delivery Systems: A Systematic Review. American Journal of Preventive Medicine 52:2, pages e33-e66.
Crossref
Maurice R. Smith, Bruce Clark, Frank Lüdicke, Jean-Pierre Schaller, Patrick Vanscheeuwijck, Julia Hoeng & Manuel C. Peitsch. (2016) Evaluation of the Tobacco Heating System 2.2. Part 1: Description of the system and the scientific assessment program. Regulatory Toxicology and Pharmacology 81, pages S17-S26.
Crossref
Xiang Li. (2016) In vitro toxicity testing of cigarette smoke based on the air-liquid interface exposure: A review. Toxicology in Vitro 36, pages 105-113.
Crossref