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Human Vaccines & Immunotherapeutics Volume 11, 2015 - Issue 2
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Research Paper

Characterization of a recombinant humanized anti-cocaine monoclonal antibody and its Fab fragment

Terence L KirleyDepartment of Pharmacology and Cell Biophysics; College of Medicine; University of Cincinnati; Cincinnati, OHUSACorrespondence[email protected]
&
Andrew B NormanDepartment of Pharmacology and Cell Biophysics; College of Medicine; University of Cincinnati; Cincinnati, OHUSA
Pages 458-467 | Received 30 Jul 2014, Accepted 04 Oct 2014, Published online: 31 Mar 2015

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Rose P. Webster, Jordan A. Marckel & Andrew B. Norman. (2023) Toxicokinetics of a humanized anti-cocaine monoclonal antibody in male and female rats and lack of cross-reactivity. Human Vaccines & Immunotherapeutics 19:3.
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Marco Pravetoni. (2016) Biologics to treat substance use disorders: Current status and new directions. Human Vaccines & Immunotherapeutics 12:12, pages 3005-3019.
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Articles from other publishers (23)

Terence L. Kirley & Andrew B. Norman. (2024) Novel partial reduction of the humanized anti-cocaine mAb h2E2 for selective cysteine labeling. Biochemical and Biophysical Research Communications 692, pages 149362.
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Terence L. Kirley & Andrew B. Norman. (2023) Characterization and optimization of fluorescein isothiocyanate labeling of humanized h2E2 anti-cocaine mAb. Biochemistry and Biophysics Reports 35, pages 101520.
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Terence L. Kirley & Andrew B. Norman. (2022) Isothermal titration calorimetry determination of thermodynamics of binding of cocaine and its metabolites to humanized h2E2 anti-cocaine mAb. Biochemistry and Biophysics Reports 32, pages 101354.
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Terence L. Kirley & Andrew B. Norman. (2022) Critical evaluation of fluorescent dyes to evaluate the stability and ligand binding properties of an anti-cocaine mAb, h2E2. Journal of Immunological Methods 508, pages 113323.
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Terence L. Kirley, Kenneth D. Greis & Andrew B. Norman. (2022) Tyrosine nitration of a humanized anti-cocaine mAb differentially affects ligand binding of cocaine and its metabolites. Biochemistry and Biophysics Reports 30, pages 101278.
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Terence L. Kirley, Andrew B. Norman & Kenneth D. Greis. (2022) Oxidation of specific tryptophan residues inhibits high-affinity binding of cocaine and its metabolites to a humanized anticocaine mAb. Journal of Biological Chemistry 298:3, pages 101689.
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Terence L. Kirley & Andrew B. Norman. (2021) Cocaine binding to the Fab fragment of a humanized anti-cocaine mAb quantitated by dye absorption and fluorescence spectroscopy. Journal of Immunological Methods 496, pages 113103.
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Terence L. Kirley & Andrew B. Norman. (2021) Ligand binding to a humanized anti-cocaine mAb measured by dye absorption spectroscopy. Biochemical and Biophysical Research Communications 535, pages 93-98.
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Terence L. Kirley & Andrew B. Norman. (2020) Multi-domain unfolding of the Fab fragment of a humanized anti-cocaine mAb characterized by non-reducing SDS-PAGE. Biochemical and Biophysical Research Communications 533:3, pages 580-585.
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Terence L. Kirley & Andrew B. Norman. (2020) Ligand binding to a humanized anti-cocaine mAb detected by non-reducing SDS-PAGE. Biochemistry and Biophysics Reports 23, pages 100795.
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Terence L. Kirley, Andrew B. Norman & Hanna N. Wetzel. (2020) A novel differential scanning fluorimetry analysis of a humanized anti-cocaine mAb and its ligand binding characteristics. Journal of Immunological Methods 476, pages 112676.
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Kemin Tan, Min Zhou, Angela J. Ahrendt, Norma E. C. Duke, Nassif Tabaja, William J. Ball, Terence L. Kirley, Andrew B. Norman, Andrzej Joachimiak, Marianne Schiffer, Rosemarie Wilton & P. Raj Pokkuluri. (2019) Structural analysis of free and liganded forms of the Fab fragment of a high-affinity anti-cocaine antibody, h2E2. Acta Crystallographica Section F Structural Biology Communications 75:11, pages 697-706.
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Terence L. Kirley, Kenneth D. Greis & Andrew B. Norman. (2018) Domain unfolding of monoclonal antibody fragments revealed by non-reducing SDS-PAGE. Biochemistry and Biophysics Reports 16, pages 138-144.
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M. A. Myagkova & V. S. Morozova. (2018) Vaccines for substance abuse treatment: new approaches in the immunotherapy of addictions. Russian Chemical Bulletin 67:10, pages 1781-1793.
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Terence L. Kirley & Andrew B. Norman. (2018) Unfolding of IgG domains detected by non-reducing SDS-PAGE. Biochemical and Biophysical Research Communications 503:2, pages 944-949.
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Hanna N. Wetzel, Tongli Zhang & Andrew B. Norman. (2017) A mathematical model of a recombinant humanized anti-cocaine monoclonal antibody's effects on cocaine pharmacokinetics in mice. Life Sciences 184, pages 81-86.
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Hanna N. Wetzel, Cinder Cohen, Andrew B. Norman & Rose P. Webster. (2017) A novel Python program for implementation of quality control in the ELISA. Journal of Immunological Methods 448, pages 80-84.
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Hanna N. Wetzel, Rose P. Webster, Fatima O. Saeed, Terence L. Kirley, William J. Ball & Andrew B. Norman. (2017) Characterization of a recombinant humanized anti-cocaine monoclonal antibody produced from multiple clones for the selection of a master cell bank candidate. Biochemical and Biophysical Research Communications 487:3, pages 690-694.
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Sasa Chu, Xuhui Zhu, Na You, Wei Zhang, Feng Zheng, Binggang Cai, Tingting Zhou, Yiwen Wang, Qiannan Sun, Zhiguo Yang, Xin Zhang, Changjun Wang, Shinan Nie, Jin Zhu & Maorong Wang. (2016) The Fab Fragment of a Human Anti-Siglec-9 Monoclonal Antibody Suppresses LPS-Induced Inflammatory Responses in Human Macrophages. Frontiers in Immunology 7.
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Hanna N. Wetzel, Vladimir L. Tsibulsky & Andrew B. Norman. (2016) The effects of a repeated dose of a recombinant humanized anti-cocaine monoclonal antibody on cocaine self-administration in rats. Drug and Alcohol Dependence 168, pages 287-292.
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Terence L. Kirley, Kenneth D. Greis & Andrew B. Norman. (2016) Selective disulfide reduction for labeling and enhancement of Fab antibody fragments. Biochemical and Biophysical Research Communications 480:4, pages 752-757.
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Terence L. Kirley, Kenneth D. Greis & Andrew B. Norman. (2016) Structural characterization of expressed monoclonal antibodies by single sample mass spectral analysis after IdeS proteolysis. Biochemical and Biophysical Research Communications 477:3, pages 363-368.
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Hanna N. Wetzel, William J. Ball & Andrew B. Norman. 2016. Biologics to Treat Substance Use Disorders. Biologics to Treat Substance Use Disorders 109 117 .

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