Anti-inflammatory effects of the protein hydrolysate and peptide fractions isolated from Salvia hispanica L. seeds

Figures & data

Figure 1. Effect of protein derivatives from S. hispanica L. seeds on cell viability. The results represent the mean ± SD of three independent experiments in triplicate. Data were analyzed using one-way ANOVA followed by Dunnett’s post hoc test. Levels of p < 0.05 were considered as statistically significant in contrast to C(₋). C(₋): negative control, C(+): positive control, H: protein hydrolysate, F: peptide fraction.

Figure 2. Effect of protein derivatives from S. hispanica L. seeds on NO (A) and H₂O₂ (B) production. The results represent the mean ± SD of three independent experiments in triplicate. Data were analyzed using one-way ANOVA followed by Dunnett’s post hoc test. Levels of p < 0.05 were considered as statistically significant in contrast to C(+). C(+): positive control, C(₋): negative control, H: protein hydrolysate, F: peptide fraction.

Figure 3. Effect of protein derivatives from S. hispanica L. seeds on IL-1β (A), IL-6 (B), TNF-α (C) and IL-10 (D) production. The results represent the mean ± SD of three independent experiments in triplicate. Data were analyzed using one-way ANOVA followed by Dunnett’s post hoc test. Levels of p < 0.05 were considered as statistically significant in contrast to C(+). C(+): positive control, C(₋): negative control, H: protein hydrolysate, F: peptide fraction.

Figure 4. Effect of 1–3 kDa fraction from S. hispanica L. seeds on TPA-induced ear edema. The results represent the mean ± SE of each group. Data were analyzed using two-way ANOVA followed by Dunnett’s post hoc test. Levels of p < 0.05 were considered as statistically significant in contrast to C(₋). C(₋): negative control group, C(+): positive control group, F1-3 (100 µg/kg): experimental group.

Figure 5. Effect of 1–3 kDa fraction from S. hispanica L. seeds on DNFB-induced delayed hypersensitivity assay. The results represent the mean ± SE of each group. Data were analyzed using two-way ANOVA followed by Dunnett’s post hoc test. Levels of p < 0.05 were considered as statistically significant in contrast to C(₋). C(₋): negative control group, C(+): positive control group, F1-3 (100 µg/kg): experimental group.