Enhanced Antioxidative Activity of Chinese Traditionally Fermented Okara (Meitauza) Prepared with Various Microorganism

Figures & data

Figure 1 Flow program of Meitauza processing.

Table 1 Methanol extract yield of various Meitauza kojis (%)

Starter organism	Extraction yield (%)
Control (non-fermented)	3.14
Fermented with
B. Subtilis B2	9.20
R. oligosporus	6.90
A. elagens	5.35
A. oryzae	13.03

Figure 2 ABTS radical scavenging activity of various extracts of Meitauza koji. Values represent the means + standard deviation (SD) of n = 3 duplicate assays.

Table 2 IC₅₀ of Meitauza koji extracts and relative antioxidative effects of Meitauza kojis in ABTS and DPPH radical scavenging activities

Meitauza koji	ABTS		DPPH
	IC50(mg/mL) of extract ^a	Relative effects of Meitauza koji ^b	IC50(mg/mL) of extract	Relative effects of Meitauza koji
Control (non-fermented)	0.248 ± 0.017A ^c	1.0	3.62 ± 0.030A	1.00
Fermented with
B. Subtilis	0.058 ± 0.001B	12.53	1.34 ± 0.017B	7.92
R. oligosporus	0.135 ± 0.001C	4.04	1.53 ± 0.024C	5.34
A. elagens	0.132 ± 0.006C	3.20	1.58 ± 0.063C	3.25
A. oryzae	0.183 ± 0.002D	5.62	2.09 ± 0.031D	7.19
^aIC50: the efficient concentration of antioxidant decreasing initial DPPH or ABTS radical concentration by 50%. IC50 was obtained by interpolation from linear regression analysis.^bRelative antioxidative effect of Meitauza koji were obtained by dividing extraction rate with IC50 value and compared with that of non-fermented okara, which was assigned as 1.0.^cIC50 values are given as mean SD (n = 3) and means with different letters for the same column are significantly different (p < 0.05).

Figure 3 DPPH radical scavenging activity of various extracts of Meitauza koji. Values represent the means + standard deviation (SD) of n = 3 duplicate assays.

Figure 4 Reducing power of various extracts of Meitauza koji. Values represent the means + standard deviation (SD) of n = 3 duplicate assays.

Figure 5 Change of peptide content of various extracts of Meitauza koji. Values represent the means + standard deviation (SD) of n = 3 duplicate assays.