Importance of Deoxyribose Degradation Assay for Evaluating Hydroxyl Radical Scavenging Activity of Punica Extract

Figures & data

Table 1 Reagents (A–D) for measuring hydroxyl radical scavenging activity

Solution No.	Composition
A	3.75 mM deoxyribose (50.0 mg), 1 mM H2O2 (10 μL), 100 μM FeCl3 (1.6 mg), 100 μM EDTA (3.7 mg), 100 μM ascorbic acid (1.761 mg) dissolved in potassium phosphate buffer
B	3.75 mM deoxyribose (50.0 mg), 1 mM H2O2 (10 μL), 100 μM FeCl3 (1.6 mg), 100 μM ascorbic acid (1.761 mg) dissolved in potassium phosphate buffer. It lacks EDTA in solution.
C	3.75 mM deoxyribose (50.0 mg), 1 mM H2O2 (10 μL), 100 μM FeCl3 (1.6 mg), 100 μM EDTA (3.7 mg) dissolved in potassium phosphate buffer. It lacks ascorbic acid in solution.
D	3.75 mM deoxyribose (50.0 mg), 1 mM H2O2 (10 μL), 100 μM FeCl3 (1.6 mg) dissolved in potassium phosphate buffer. It lacks both EDTA and ascorbic acid in solution.

Figure 1 Concentration-dependent hydroxyl radical scavenging effect of seed and peel extracts of Punica granatum L. (a) In presence of EDTA but absence of ascorbic acid; (b) In absence of both EDTA and ascorbic acid; (c) In presence of ascorbic acid but absence of EDTA; (d) In presence of both EDTA and ascorbic acid. PPW: Punica peel aqueous extract; PPE: Punica peel ethanol extract; PSE: Punica seed ethanol extract; PSW: Punica seed aqueous extract. Values are mean ± SE of three independent analysis.