Antioxidant and radical scavenging activities of Asphodelus aestivus Brot. extracts

Figures & data

Table 1 The total flavonoid, total chlorophyll, and total carotenoid contents of extracts from Asphodelus aestivus Brot. leaves

Sample	Chlorophyll a (μg/ml) ^a	Chlorophyll b (μg/ml) ^a	Total Chlorophyll (μg/ml) ^a	Total Carotenoid (μg/ml) ^a	Total Flavonoid Content (μg catechin/mg of extract) ^a
Methanol extract	1.51 ± 0.020	2.88 ± 0.070	4.39 ± 0.030	0.12 ± 0.002	2.30 ± 0.020
Acetone extract	10.26 ± 0.720	6.10 ± 0.003	16.36 ± 0.720	1.73 ± 0.008	17.74 ± 0.460
^aValues were the means of three replicates ± standard deviation (SD).

Table 2 β-Carotene bleaching test. ^a BHA was used as reference antioxidant

Sample	RAA ^b 60 min	RAA 120 min
Methanol extract	1.77 ± 0.05	1.24 ± 0.02
Acetone extract	1.66 ± 0.04	1.24 ± 0.03
Negative control ^c	0.58 ± 0.02	0.36 ± 0.05
^aValues were the means of three replicates ± standard deviation (SD).
^bRelative antioxidant activities.
^cNegative control: linoleic acid and β-carotene emulsion.

Figure 1 Reducing power of the extracts from Asphodelus aestivus Brot. leaves. α-Tocopherol, BHA, and BHT were used as reference antioxidants. Values are means ± SD (n = 3).

Figure 2 DPPH radical scavenging activity of the extracts from Asphodelus aestivus Brot. leaves. α-Tocopherol, ascorbic acid, BHA, and BHT were used as reference antioxidants. Values are means ± SD (n = 3).

Table 3 ABTS^•+ scavenging ability of the extracts from Asphodelus aestivus Brot. leaves. The results were expressed as μmol Trolox equivalent at 20, 40, 60, 80, and 100 μg/ml of extract, respectively

	ABTS^•+ scavenging ability (μmol trolox / μg of extract) ^a
Sample	20 μg/ml	40 μg/ml	60 μg/ml	80 μg/ml	100 μg/ml
Methanol extract	298.28 ± 1.85	299.42 ± 0.46	306.45 ± 1.74	315.06 ± 1.41	318.90 ± 0.68
Acetone extract	299.43 ± 1.44	304.06 ± 3.17	310.08 ± 1.13	312.55 ± 1.26	318.26 ± 1.56
^aValues were the means of three replicates ± standard deviation (SD).

Figure 3 Total antioxidant activities of methanolic and acetone extracts from Asphodelus aestivus Brot. leaves measured by ferric thiocyanate method. α-Tocopherol, BHA, and BHT were used as reference antioxidants. Values are means ± SD (n = 3).

Figure 4 Superoxide radical scavenging activity of the extracts from Asphodelus aestivus Brot. leaves. BHA, trolox, and epicatechin were used as reference antioxidants. Values are means ± SD (n = 3).