Effect of Detoxification on Conformational and Functional Properties of Jatropha curcas Proteins

Figures & data

FIGURE 1 (a) SDS-PAGE profiles. Line M, the marker proteins; line 1, JMP; line 2, DJMP. (b) Molecular weight distribution of DJMP.

TABLE 1 Effect of detoxification on H₀, ZP, thermal characteristics, free sulfhydryl contents, and disulfide bond contents of DJMP and JMP

	Thermal characteristics (DSC)		Free sulfhydryl and disulfide bond contents (μmol/g protein)
	Td (°C)	ΔH (J/g)	Total	Exposed	Disulfide bond	H0	ZP
DJMP	79.20 (0.98)	3.31 (0.12)	19.30 (1.71)	5.26 (0.37)	7.02 (1.01)	680.24 (10.94)	−11.73 (1.01)
JMP	108.88 (2.17)	20.09 (1.01)	31.33 (2.47)	10.27 (0.95)	10.53 (1.60)	374.24 (4.97)	−9.66 (1.02)

All values are means of triplicate determinations (standard deviation).

TABLE 2 Effect of detoxification on the secondary structure of DJMP and JMP by FT-IR spectra

	Composition of the secondary structure
	α-Helix	β-Sheet				β-Turn		Random coil
Peak/cm^−1	1650	1612	1621	1631	1641	1678	1688	1659	1668
DJMP (%)	19.53	3.36	11.32	8.18	9.19	12.22	8.60	19.39	8.31
			Total: 32.05			Total: 20.82		Total: 27.70
JMP (%)	18.60	3.53	11.51	8.80	9.80	6.00	9.04	23.31	9.41
			Total: 33.64			Total: 15.04		Total: 32.72

TABLE 3 Effect of detoxification on functional properties of DJMP, JMP, and SPI

	DJMP	JMP	SPI
PS (%)	80.41 (0.01)	42.70 (0.01)	81.00 (0.02)
WHC (g/g)	2.16 (0.06)	1.65 (0.007)	4.84 (0.05)
FHC (g/g)	3.20 (0.01)	2.69 (0.03)	1.94 (0.02)
EA	0.32 (0.004)	0.21 (0.002)	0.59 (0.003)
ES (%)	91.36 (0.26)	83.21 (0.10)	97 (0.04)
FC (%)	64.25 (0.04)	50.75 (0.01)	39.13 (0.85)
FS	0.48 (0.02)	0.16 (0.01)	0.99 (0.01)

All values are means of triplicate determinations (standard deviation).