In Vitro Antioxidant and Antiglycation Properties of Methanol Extract and Its Different Solvent Fractions of Musa paradisiaca L. (Cv. Nendran) Inflorescence

Figures & data

Table 1  Preliminary phytochemical screening of methanolic extract and different solvent fractions of Musa paradisiacal inflorescence

Test	ME	PE	EA	BU	WA
Phenolics	++	+	+++	+	++
Flavanoids	+	−	+++	−	−
Terpenoids	++	+	+	−	−
Alkaloids	++	−	−	+	++
Tannins	++	−	+	+	++
Steroids	++	−	++	−	−
Saponins	+	−	−	−	++
Reducing sugars	++	−	+	+	++
− Absent; + present; ++ traces; +++ abundant.

Table 2  IC₅₀ values of DPPH radical, ABTS radical, superoxide radical, hydroxyl radical, and nitric oxide radical, scavenging activities of Musa paradisiaca inflorescence [IC₅₀ value μg/ml (mean ± SD)]

Sample	DPPH radical scavenging	ABTS radical scavenging	Super oxide radical scavenging	Hydroxyl radical scavenging	Nitric oxide radical scavenging
ME	28.50 ± 1.30	35.50 ± 2.40	44.70 ± 3.10	39.70 ± 3.70	43.00 ± 1.50
PE	65.00 ± 4.80	96.80 ± 5.20	105.00 ± 6.80	85.80 ± 5.80	113.00 ± 4.10
EA	9.80 ± 0.80	13.50 ± 1.40	26.40 ± 3.20	19.71 ± 2.70	25.73 ± 2.20
BU	61.00 ± 1.60	73.00 ± 5.60	112.00 ± 5.90	63.50 ± 3.50	80.00 ± 3.60
WA	52.00 ± 2.90	69.00 ± 4.70	97.00 ± 4.80	75.40 ± 2.80	103.00 ± 4.90
GA	1.50 ± 0.90	—	—	—	—
TRX	—	8.50 ± 1.10	—	—	—
ASC	—	—	15.50 ± 2.10	—	—
CAT	—	—	—	10.26 ± 1.50	—
CUR	—	—	—	—	17.00 ± 0.98
ME: Methanol extract; PE: petroleum ether fraction; EA: ethyl acetate fraction; BU: butanol fraction; WA: water fraction; GA: gallic acid; TRX: trolox; ASC: ascorbic acid; CAT: catechin; CUR: curcumin.

Figure 1 TPC and TFC of the ME and its different fractions of Musa paradisiaca inflorescence. Units: total phenolic content expressed as mg GAE/g dry weight; total flavonoid content expressed as mg CE/g dry weight. ME: methanolic extract; PE: petroleum ether fraction; EA: ethyl acetate fraction; BU: butanol fraction; WA: water fraction.

Figure 2 TRP of ME and its different fractions of Musa paradisiaca inflorescence. ME: methanolic extract; PE: petroleum ether fraction; EA: ethyl acetate fraction; BU: butanol fraction; WA: water fraction.

Figure 3 Inhibition of protein glycation (AGE formation) by Musa paradisiaca. Results are expressed as percentage of inhibition of AGE formation in IC₅₀ (μg/ml). ME: methanolic extract; PE: petroleum ether fraction; EA: ethyl acetate fraction; BU: butanol fraction; WA: water fraction.