Preparation and Characterization of Nanoscale Complex Liposomes Containing Medium-Chain Fatty Acids and Vitamin C

Figures & data

FIGURE 1 TEM picture of MCFAs-Vit.C complex liposomes by double emulsion. The bar represents 200 nm.

FIGURE 2 TEM picture of MCFAs-Vit.C nanoscale complex liposomes by double emulsion-dynamic high pressure microfluidization. The bar represents 100 nm.

TABLE 1 Physicochemical characteristics of MCFAs-Vit.C complex liposomes at 4°C

Days	Mean diameter (nm)	EEMCFAs (%)	EEVit.C (%)
1	95.5 ± 5.86^a	48.66 ± 3.14	64.19 ± 2.59
4	98.8 ± 4.68^a	46.11 ± 2.98	65.87 ± 1.68
7	104 ± 6.87^a, b	42.28 ± 3.05	64.87 ± 1.58
10	107 ± 6.38^a, b	44.01 ± 3.25	62.41 ± 1.63
13	108 ± 6.98^a, b	41.15 ± 2.68	63.91 ± 1.75
16	106 ± 5.96^a, b	40.91 ± 3.68	62.79 ± 2.04
30	119 ± 5.37^b, c, d	43.75 ± 2.87	60.95 ± 2.68
45	125 ± 6.21^c, d, e	41.06 ± 3.17	57.62 ± 3.52
60	130 ± 4.53^d, e	42.52 ± 1.77	59.28 ± 1.78
90	138.3 ± 5.26^e	40.39 ± 1.86	58.28 ± 3.08

Data are means ±SD for thee different experiments. Values bearing the same letters within the same raw are not significantly different from each other (P < 0.05).

FIGURE 3 In vitro drug release of Vit.C from liposomes at 37°C (n = 3, ±SD). □: The percentage of Vit.C release in Vit.C liposomes; ▪: the percentage of Vit.C release in MCFAs-Vit.C nanoscale complex liposomes; ▴: the percentage of Vit.C release in solution containing Vit.C.

FIGURE 4 In vitro drug release of MCFAs from liposomes at 37°C (n = 3, ±S D). ♦: The percentage of MCFAs release in MCFAs liposomes; ▪: the percentage of MCFAs release in MCFAs-Vit.C nanoscale complex liposomes; ▴: the percentage of MCFAs release in solution containing MCFAs.