Analysis of Polyphenols in Apple Pomace using Gas Chromatography-Mass Spectrometry with Derivatization

Figures & data

Figure 1 Effect of BSTFA sample volume on the gas chromatogram of the phenolic compounds found in apple pomace. (a) Mass ratio of sample to BSTFA is 1:10; (b) mass ratio of sample to BSTFA is 1:20; (c) control.

Figure 2 Effect of different reaction temperatures on the gas chromatogram of the phenolic compounds found in apple pomace. (a) Room temperature (20°C); (b) 37.5°C; (c) control.

Figure 3 Effect of reaction time on the gas chromatogram of the phenolic compounds found in apple pomace. (a) 30 min; (b) 12 h; (c) control.

Figure 4 Total ion chromatogram of the phenolic compounds found in apple pomace after derivatization.

Table 1 Phenolic compounds and organic acids found in apple pomace

Peak	Retention time/min	Polyphenolic compounds	Formula	Peak area /%
1	5.196	1,3-phenyl-1-acetone	C15H14O	0.61
4	11.133	N-(phenyl) methylene-β, 3,4-trihydroxy-	C15H15NO3	5.68
6	13.622	phenylethylamine	C3H3N3O3	3.48
8	14.405	2,4,6-trihydroxy-1, 3,5-triazine	C6H14O6	0.40
9	14.815	d-mannitol	C6H12O6	0.48
10	15.594	d-mannose pyran	C16H32O2	3.29
14	17.396	Palmitic acid	C18H36O2	0.85
19	21.202	Eighteen carbonate	C15H14O6	5.08
21	21.779	Epicatechin	C15H14O6	2.09
23	24.034	Epicatechin	C15H14O6	7.31
24	24.492	Epicatechin	C15H14O6	7.74