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Original Articles

Influence of Pulsed Electric Field on Escherichia coli and Saccharomyces cerevisiae

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Pages 1416-1427 | Received 21 Nov 2013, Accepted 17 Apr 2014, Published online: 11 Mar 2015

Figures & data

FIGURE 1 PEF processing.

FIGURE 1 PEF processing.

FIGURE 2 Inactivation of PEF on E. coli and S. cerevisiae: (a) E. coli, electric field strength, treatment time; (b) S. cerevisiae, electric field strength, treatment time; (c) E. coli, electric field strength, conductivity; (d) S. cerevisiae, electric field strength, conductivity.

FIGURE 2 Inactivation of PEF on E. coli and S. cerevisiae: (a) E. coli, electric field strength, treatment time; (b) S. cerevisiae, electric field strength, treatment time; (c) E. coli, electric field strength, conductivity; (d) S. cerevisiae, electric field strength, conductivity.

FIGURE 3 SEM image of PEF-treated cells of S. cerevisiae: (a) control; (b) PEF-treated cells (30 kV/cm, 60 μs); (c) PEF-treated cells (35 kV/cm, 90 μs).

FIGURE 3 SEM image of PEF-treated cells of S. cerevisiae: (a) control; (b) PEF-treated cells (30 kV/cm, 60 μs); (c) PEF-treated cells (35 kV/cm, 90 μs).

FIGURE 4 PEF-treated cells of S. cerevisiae observed using TEM: (a) control; (b) PEF-treated cells (30 kV/cm, 60 μs); (c) PEF-treated cells (35 kV/cm, 90 μs).

FIGURE 4 PEF-treated cells of S. cerevisiae observed using TEM: (a) control; (b) PEF-treated cells (30 kV/cm, 60 μs); (c) PEF-treated cells (35 kV/cm, 90 μs).

TABLE 1 Amount of UV-absorbing materials from S. cerevisiae leaked into the suspension after PEF treatment

TABLE 2 FCM result of S. cerevisiae after PEF treatment

FIGURE 5 FCM result of S. cerevisiae after PEF treatment.

FIGURE 5 FCM result of S. cerevisiae after PEF treatment.

FIGURE 6 Effect of PEF treatment on the DNA denaturation in S. cerevisiae cells. Upper left and lower right corners represent red and green fluorescence, respectively. The upper right corner represents the red and green fluorescent coexisting region.

FIGURE 6 Effect of PEF treatment on the DNA denaturation in S. cerevisiae cells. Upper left and lower right corners represent red and green fluorescence, respectively. The upper right corner represents the red and green fluorescent coexisting region.

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