In Vitro Dissolution of Calcium Carbonate from the Chicken Eggshell: A Study of Calcium Bioavailability

Figures & data

TABLE 1 Digestion program of eggshell samples

Step	Initial power, W	End power, W	Ramp time, min
1	100	300	5
2	0	0	1
3	300	600	5
4	0	0	1
5	600	800	5
6	0	0	1
7	800	1000	5
8	0	0	1
9	1000	1000	10
10	0	0	15 (cooling)

TABLE 2 Operating parameters of ICP OES

Parameter	Value
Plasma gas flow (L/min)	15
Auxiliary gas flow (L/min)	0.5
Gas flow through atomizer (L/min)	0.5
Plasma power (W)	1450
Plasma observation height (mm)	15
Sample flow (mL/min)	0.65
Delay time (sec)	45

FIGURE 1 Calibration curves for the Ca concentration analysis using the WD-XRF technique.

FIGURE 2 In vitro dissolution of calcium carbonate preparations after 10, 30, 60, and 90 minutes.

TABLE 3 In vitro dissolution of calcium carbonate preparations at 10, 30, 60, and 90 minutes

	10	30	60	90
Iceland spar	98.3 ± 0.6	100.1 ± 0.1	98.4 ± 0.7	99.8 ± 1.2
Precipitated CaCO3	50.3 ± 0.2	66.2 ± 2.8	70.9 ± 0.9	80.1 ± 1.3
Oyster shell A	41.0 ± 1.8	62.7 ± 3.3	71.7 ± 3.6	79.1 ± 2.3
Oyster shell B	19.5 ± 1.7	26.4 ± 0.6	29.4 ± 1.6	31.4 ± 2.0
CE before incubation	78.3 ± 10.1	84.2 ± 7.3	89.5 ± 4.9	93.2 ± 4.8
CE after incubation	57.5 ± 9.2	75.5 ± 9.5	86.8 ± 5.6	93.3 ± 3.2