Phytochemistry, Antioxidant, and Lipid Peroxidation Inhibition of the Essential Oils of Lavandula Officinalis L. in Iran

Figures & data

Table 1. Chemical compositions of Lavandula officinalis.

	Components	%
1	Linalool oxide	0.34
2	α-Pinene	20.14
3	α-Terpinene	0.87
4	Borneol	3.45
5	Camphor	14.36
6	Menthol	32.51
7	Eucarvone	0.45
8	Terpinolene	0.71
9	β-Caryophyllene	0.14
10	Viridiflorol	0.7
11	1,8-Cineole	20.14
12	β-Pinen	1.36
13	Linalool	1.36
	Total	96.53

RI: Retention indices relative to C₈–C₂₅ n-alkanes on the HP-5 column.

Table 2. Extraction yields, total phenolic contents, and total flavonoid contents of L. officinalis extracts.

Extract	Extraction yield^a	Total phenolic^b	Total flavonoid^c
L. officinalis	121.7 ± 07	164.75 ± 01	191.27 ± 02

The data are expressed as mean ± SD;

^aExpressed as mg of extract per gram dry material;

^bExpressed as mg of gallic acid per gram dry extract;

^cExpressed as mg of rutin per gram dry extract (water).

Table 3. Antioxidant activity of EO extract from L. officinalis: DPPH free radical scavenging activity (expressed as IC₅₀ values: µg/mL), and β-carotene bleaching test. Reducing power was expressed as EC₅₀ values (µg/mL). Butylhydroxyanisole and ascorbic acid were used as positive controls.

Tested compounds	IC50 (μg/mL)
L. officinalis EO	12.07 ± 0.31 µg/mL
α-Pinene	14.07 ± 0.11 µg/mL
Camphor	12.18 ± 0.65 µg/mL
Menthol	11.54 ± 0.04 µg/mL
1,8-Cineole	11.04 ± 0.31 µg/mL
L. officinalis EO (β-Carotenes IC50 µg/mL)	12.84 ± 0.11 µg/mL
L. officinalis EO (Reducing power EC50 µg/mL)	11.69 ± 0.04 µg/mL
BHA (IC50 µg/mL)	12.00 ± 0.01 µg/mL
AA (IC50 µg/mL)	12.07 ± 0.08 µg/mL

Values are mean ± S.D. of three replications;

*IC50 values have been presented with their respective 95% confidence limits.

Table 4. Components identified and their antioxidant activity relative percentages.

Compounds	%
α-Pinene	3
Camphor	5
Menthol	39
1,8-Cineole	48

Values are given as mean ± SD (n = 3);

Means in each column followed by different letters are significantly different (p < 0.05).

Table 5. Lipid peroxidation inhibition of EO extract from L.officinalis and its main samples (expressed as IC₅₀ values: µg/mL). Trolox and ascorbic acid were used as positive controls.

Tested compounds	IC50 (μg/mL)
L. officinalis EO	82.21 ± 0.35 µg/mL
α-Pinene	61.21 ± 0.03 µg/mL
Camphor	75.21 ± 0.07 µg/mL
Menthol	89.21 ± 0.09 µg/mL
1,8-Cineole	91.01 ± 0.04 µg/mL
Trolox	85.21 ± 0.02 µg/mL
AA	84.14 ± 0.06 µg/mL

Experiments were carried out in triplicate and the results are expressed as mean ± SD.