Chemical composition and bioactivities of the polyphenolic-rich extract of Ormenis africana Jord. and Fourr

Figures & data

Table 1. Peak assignments of the methanol extract of S. africana.

Peak No.	RT (min)	UV λmax (nm)	[M+H]^+ (m/z)	[M-H]^− (m/z)	Identification	Occurrence
1	8.35	244, 290sh, 326	355.23	353.15	mono-Caffeoylquinic acid
2	8.53	325	188/205	−	Unknown
3	8.9	244, 290sh, 326	355	353.15	mono-Caffeoylquinic acid
4	11.09	244, 290sh, 326	355.23	353.15	Chlorogenic acid*	++++
5	13.01	271, 328	595/433	593.13/431/269	Apigenin di-glucoside	+
6	15.8	322	−	179	Caffeic acid
7	18.2	−	−	305	Unknown	+
8	19.2	350	−	225	Unknown
9	20.05	308	803 ([M+Na]^+)	779.3	Unknown	+
10	24.48	264,328	595/433	593.13/269	Apigenin di-glucoside	+++
11	25.97	264, 348	465	463/285	kaempferol glucoside
12	27.23	246, 348	449/287	447.27/285	Luteolin 7-O-β-D-glucoside*	+++
13	31.4	268, 329	579	577.12/431/269	Apigenin 7-O-rutinoside*	+
14	33.5	244, 290sh, 326	517.27	515.06	3,5-O-di-Caffeoylquinic acid*	++++
15	34.5	244, 290sh, 326	517.27	515.06	di-Caffeoylquinic acid isomer
16	35.04	266, 334	433	431/269	Apigenin 7-O-β-D-glucoside
17	37.54	244, 290sh, 326	517.27	515.13	di-Caffeoylquinic acid isomer	+

*: identified by comparison with a standard reference

Figure 1. LC-DAD-TIC chromatogram of Tunisian S. africana extract. The peak assignments are listed in Table 1.

Table 2. α-Glucosidase and α-amylase inhibition activity (%) of S. africana extract.

Sample	α-amylase inhibition	α-glucosidase inhibition
MeOH extract (10 µg/mL)	12 ± 0.22 ^d	8 ± 0.10 ^e
MeOH extract (20 µg /mL)	31 ± 0.54 ^c	37 ± 0.09 ^d
MeOH extract (40 µg /mL)	64 ± 0.32 ^b	61 ± 0.14 ^c
MeOH extract (60 µg /mL)	92 ± 0.41 ^a	81 ± 0.21 ^b
MeOH extract (80 µg /mL)	97 ± 0.11^a	90 ± 0.17 ^a
MeOH extract (100 µg /mL)	−	98 ± 0.20^a
Acarbose (20 µg /mL)	67 ± 0.19^b	69 ± 0.33 ^c

Results are reported as mean ± standard deviation of three replicates. Values in the same column followed by the same letter are not significantly different (Duncan’s multiple range test at p >0.05).

Table 3. Antioxidant capacity of the MeOH extract of Santolina africana.

	Antioxidant Capacity
Sample	TRP^a	ABTS^b	DPPH^b	FRAP^b
Methanolic extract	50 ± 0.408	36 ± 2	46 ± 0.5	52 ± 3.1

Results are reported as mean ± standard deviation of three replicates

^a: mg gallic acid equivalents/g dry weight; ^b: µm Trolox equivalents (TE).