A species specific simplex polymerase chain reaction-based approach for detection of goat tallow in heat clarified milk fat (ghee)

Figures & data

Table 1 Effect of different concentration of MgCl₂ on the specificity of the goat specific primer G.

Concentration of MgCl2	1.5 mM	2.5 mM	3.5 mM	4.5 mM
Cross amplification with buffalo	×	×	√	*

× = cross amplification with buffalo, √ = no cross amplification, * = no amplification, mM-millimolar.

Figure 1. Amplification of goat specific primer (G) with DNA isolated from blood of various species.

Figure 2. Goat specific primer(G) amplification with DNA isolated from fat samples.

Figure 3. Detection of goat tallow in buffalo ghee by primer G.

Figure 4. Detection of goat tallow in cow ghee by primer G.