Detection of ten biogenic amines in Chinese commercial soybean paste by HPLC

Figures & data

Table 1. Gradient elution program for biogenic amines analysis.

Time (min)	0	2	5	10	16	20	21	25	27	36
Acetonitrile (%)	60	60	38	38	20	20	0	0	60	60
Ammonium acetate solution (%)	40	40	62	62	80	80	100	100	40	40

Figure 1. Chromatogram of derivatives of biogenic amines standards (5 μg/g).

Figure 2. Chromatogram of derivatives of biogenic amines in sample 1.

Table 2. Linear ranges, calibration curves, correlation coefficient (R), method limits of detection, and quantitation (MLODs and MLOQs) of ten biogenic amines.

BA	Calibration curves	R	Linear ranges (μg/g)	MLODs (μg/g)	MLOQs (μg/g)
Put	y = 9.6368x - 0.5572	0.9969	0.1–80	0.01	0.03
Cad	y = 15.548x - 0.6264	0.9980	0.1–80	0.01	0.04
Spd	y = 13.048x - 0.5076	0.9951	0.1–80	0.01	0.04
Spm	y = 14.387x - 0.6906	0.9935	0.1–80	0.01	0.03
Tyr	y = 15.981x - 0.6751	0.9971	0.1–80	0.01	0.04
Phe	y = 19.275x - 0.9176	0.9905	0.1–80	0.01	0.03
His	y = 12.766x - 0.0525	0.9981	0.1–80	0.03	0.10
Ser	y = 9.0984x + 1.6702	0.9909	0.1–80	0.03	0.10
Try	y = 22.662x - 0.1399	0.9901	0.1–80	0.03	0.09
Agm	y = 10.027x + 0.2399	0.9934	0.1–80	0.03	0.10

Note: In the linear equations, x represents the ratio of the standard peak area to the internal standard peak area; y represents the concentration of standard solutions (μg/mL).

Table 3. Contents of biogenic amines in soybean paste samples.

Samples	Biogenic amines^a
	Put	Cad	Spd	Spm	Try	Phe	His	Ser	Tyr	Agm
Sp1	22.60 ± 1.53	2.54 ± 1.66	5.24 ± 0.48	0.54 ± 0.08	ND^b	2.01 ± 0.51	28.29 ± 1.63	26.40 ± 1.55	37.77 ± 1.05	18.99 ± 2.81
Sp2	9.74 ± 1.05	9.18 ± 3.14	25.56 ± 0.20	3.00 ± 0.17	10.90 ± 1.28	16.57 ± 0.72	74.94 ± 4.27	10.80 ± 1.13	159.00 ± 14.79	51.76 ± 8.73
Sp3	34.49 ± 0.57	52.41 ± 0.34	10.92 ± 0.63	4.64 ± 0.50	11.07 ± 0.11	32.36 ± 0.55	44.14 ± 3.17	4.16 ± 0.53	384.06 ± 8.93	229.59 ± 6.63
Sp4	5.08 ± 8.70	0.76 ± 0.73	50.26 ± 2.58	13.33 ± 2.31	7.80 ± 0.36	57.73 ± 9.67	25.30 ± 2.23	20.40 ± 34.13	411.04 ± 6.19	206.54 ± 40.82
Sp5	9.77 ± 0.82	9.24 ± 0.01	14.29 ± 1.01	15.97 ± 0.45	7.30 ± 1.46	22.04 ± 7.01	32.69 ± 5.57	10.21 ± 0.45	191.00 ± 19.91	112.09 ± 8.69
Sp6	54.84 ± 0.90	10.90 ± 0.41	18.11 ± 0.30	1.94 ± 0.38	4.99 ± 0.89	24.68 ± 1.21	51.99 ± 0.53	14.47 ± 0.81	115.00 ± 15.02	75.04 ± 1.94
Sp7	9.55 ± 0.90	3.36 ± 0.41	15.33 ± 0.30	2.35 ± 0.38	7.34 ± 0.89	6.06 ± 1.21	27.49 ± 0.53	9.32 ± 0.81	46.30 ± 15.02	108.59 ± 1.94
Sp8	8.15 ± 2.37	3.03 ± 0.29	12.62 ± 0.74	ND	7.71 ± 0.16	5.73 ± 1.97	23.19 ± 9.84	7.01 ± 0.71	57.39 ± 3.18	61.46 ± 4.55
Sp9	18.37 ± 0.28	5.97 ± 0.89	8.93 ± 1.26	1.38 ± 0.02	2.02 ± 0.89	8.86 ± 0.80	10.33 ± 3.16	8.99 ± 3.23	46.43 ± 1.60	49.41 ± 4.66
Sp10	4.62 ± 2.27	1.19 ± 1.36	37.93 ± 2.57	8.13 ± 3.14	3.00 ± 0.37	ND	2.83 ± 9.24	17.40 ± 0.03	202.25 ± 4.84	94.00 ± 1.83
Sp11	5.90 ± 1.28	2.40 ± 0.48	44.97 ± 1.23	14.92 ± 0.08	5.49 ± 3.96	54.02 ± 1.33	129.88 ± 1.03	20.20 ± 0.25	222.92 ± 2.25	175.94 ± 0.11
Sp12	5.42 ± 0.22	27.90 ± 0.36	25.74 ± 2.25	2.52 ± 0.17	4.12 ± 0.13	28.50 ± 0.05	83.50 ± 0.25	21.40 ± 1.32	144.00 ± 10.77	154.04 ± 3.07
Sp13	1.21 ± 0.75	3.95 ± 2.99	7.38 ± 6.51	ND	4.15 ± 4.06	4.85 ± 0.06	7.27 ± 0.15	3.20 ± 2.64	36.41 ± 7.62	103.80 ± 12.02
Sp14	13.90 ± 1.66	1.16 ± 2.06	ND	ND	2.85 ± 0.26	1.91 ± 7.62	4.76 ± 9.55	10.14 ± 3.96	15.50 ± 13.71	20.50 ± 10.36
Sp15	18.55 ± 0.19	9.30 ± 0.09	8.96 ± 0.65	ND	ND	10.91 ± 0.67	5.05 ± 0.31	63.60 ± 0.37	44.04 ± 1.39	65.79 ± 2.76
Sp16	35.09 ± 0.34	4.61 ± 0.27	4.09 ± 0.36	ND	3.76 ± 0.06	2.74 ± 1.45	14.95 ± 1.55	25.75 ± 2.60	42.22 ± 2.53	60.06 ± 4.42
Sp17	19.27 ± 0.95	26.23 ± 0.72	25.17 ± 1.76	38.57 ± 0.30	73.22 ± 0.20	82.97 ± 0.14	53.76 ± 0.16	74.08 ± 1.15	142.53 ± 1.61	164.14 ± 2.87

^an = 3 mg/kg, wet weight basis.

^bPUT, CAD SPD, SPM, TRY, PHE, HIS, SER, TYR, and AGM < 0.5 mg/kg, wet weight basis. ND, not detected.