Effect of quality control on the total antioxidant capacity of the extract from Sonchus brachyotus DC.

Figures & data

Figure 1. HPLC conditions of the extract of Sonchus Brachyotus DC. Kromasil C18 column (250 × 4.6 mm i.d.; 5 μm). (A) Mobile phase; (B) organic acid; (C) 3D wavelength scan chromatogram of the extract of Sonchus Brachyotus DC.; (D) flow rate; and (E) column temperature.

Figure 2. A representative fingerprint extract of Sonchus Brachyotus DC. Peaks 1–29 were used as common characteristic peaks.

Table 1. Precision, repeatability, and stability data of the proposed method.

	Precision				Repeatability				Stability
Peak no	RRT	RSD (%)	RPA	RSD (%)	RRT	RSD (%)	RPA	RSD (%)	RRT	RSD (%)	RPA	RSD (%)
1	0.131	2.30	0.099	3.63	0.129	0.39	0.094	4.42	0.129	0.34	0.088	4.51
2	0.142	2.08	0.257	4.54	0.141	0.48	0.266	4.85	0.141	0.17	0.273	4.88
3	0.180	0.23	0.092	4.30	0.180	0.47	0.094	4.84	0.178	1.10	0.143	4.02
4	0.200	3.01	0.175	2.01	0.203	0.69	0.177	4.86	0.200	1.48	0.194	4.64
5	0.212	0.92	0.195	4.55	0.213	0.86	0.193	4.83	0.212	0.55	0.177	4.79
6	0.308	0.82	0.442	4.77	0.308	0.46	0.440	4.17	0.304	0.81	0.600	4.97
7	0.398	0.40	0.440	3.38	0.398	0.44	0.441	1.11	0.398	2.05	0.603	2.40
8	0.435	0.35	0.133	3.44	0.436	0.45	0.135	1.50	0.433	0.83	0.239	3.93
9	0.534	0.33	0.214	4.75	0.534	0.42	0.212	4.94	0.529	0.57	0.182	4.73
10	0.665	0.13	0.100	4.94	0.666	0.24	0.073	4.97	0.659	1.03	0.062	4.72
11	0.698	0.16	0.095	4.93	0.699	0.18	0.093	4.32	0.696	0.39	0.104	4.21
12(s)	1.000	0.00	1.000	0.00	1.000	0.00	1.000	0.00	1.000	0.00	1.000	0.00
13	0.783	0.18	1.183	4.77	0.785	0.54	0.849	4.94	0.789	0.91	0.545	4.98
14	0.868	0.09	0.393	2.34	0.869	0.07	0.262	4.99	0.868	0.21	0.229	4.12
15	0.947	0.07	0.238	4.84	0.948	0.04	0.199	4.99	0.946	0.25	0.219	4.36
16	0.753	0.23	2.258	3.78	0.753	0.29	2.313	2.61	0.748	0.76	2.704	4.97
17	1.051	0.14	0.395	3.05	1.051	0.10	0.277	4.59	1.050	0.15	0.214	2.29
18	1.094	0.08	0.682	3.54	1.094	0.08	0.673	4.60	1.091	0.44	0.204	4.69
19	1.175	0.07	0.277	1.92	1.175	0.08	0.286	4.94	1.179	0.66	0.126	4.90
20	1.210	0.07	6.659	3.65	1.210	0.08	6.578	4.88	1.213	0.28	6.155	4.49
21	1.240	0.06	0.868	2.35	1.240	0.08	0.839	4.68	1.244	0.27	0.501	4.92
22	1.327	0.13	0.366	3.25	1.327	0.10	0.320	4.64	1.330	0.29	0.205	4.37
23	1.350	0.13	0.141	4.85	1.350	0.11	0.127	4.86	1.353	0.31	0.113	4.90
24	1.376	0.16	0.180	4.89	1.376	0.11	0.119	4.98	1.380	0.27	0.110	4.60
25	1.395	0.17	0.505	1.19	1.394	0.14	0.507	3.44	1.397	0.31	0.366	4.11
26	1.434	0.18	8.379	3.05	1.435	0.14	7.990	4.95	1.440	0.24	6.595	4.86
27	1.533	0.22	0.252	1.01	1.530	0.28	0.214	4.93	1.528	0.28	0.157	3.97
28	1.592	0.23	1.712	2.73	1.592	0.18	1.350	4.87	1.595	0.31	1.045	4.96
29	1.816	0.27	0.215	2.54	1.815	0.20	0.138	4.83	1.816	0.36	0.082	2.31

RRT, relative retention time; RPA, relative peak area; RSD, relative standard deviation.

Figure 3. The overlay HPLC fingerprints of 10 batches samples of Sonchus Brachyotus DC. (1–10) by a similarity evaluation system that analyses sample similarity against a generated reference chromatogram (R).

Table 2. The similarities (correlation coefficients) and the TAC of 10 batches.

Sample number	Similarities	TAC (mmol/g)
Control		0.065 ± 0.008
Trolox		6.659 ± 0.032
1	0.997	0.658 ± 0.006
2	0.999	0.487 ± 0.013
3	0.999	0.450 ± 0.011
4	0.989	0.562 ± 0.008
5	0.999	0.530 ± 0.009
6	0.999	0.600 ± 0.005
7	0.993	0.637 ± 0.012
8	0.997	0.493 ± 0.010
9	0.992	0.781 ± 0.024
10	0.995	0.567 ± 0.010

All data for the TAC of 10 batches are shown as means ± SD of three independent experiments. No significant difference in the TAC of 10 batches.