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Original Article

Phenolic acids and flavonoids profiles of commercial honey from different floral sources and geographic sources

, , & ORCID Icon
Pages 290-308 | Received 28 Nov 2017, Accepted 22 Jan 2019, Published online: 04 Mar 2019

Figures & data

Table 1. Information of honey samples collected

Figure 1. High-performance liquid chromatography chromatogram of (a) standard phenolic acid solution (20 μg/mL), (b) manuka honey: 1. gallic acid, 2. protocatechuic acid, 3. 2,3,4-trihydroxybenzoic acid 4. protocatechualdehyde, 5. p-hydroxybenzoic acid, 6. gentistic acid, 7. chlorogenic acid, 8. vanillic acid + caffeic acid, 9. syringic acid, 10. vanillin, 11. p-coumaric acid + syringaldehyde 12. ferulic acid, 13. sinapic acid, 14. Salicylic

Figure 1. High-performance liquid chromatography chromatogram of (a) standard phenolic acid solution (20 μg/mL), (b) manuka honey: 1. gallic acid, 2. protocatechuic acid, 3. 2,3,4-trihydroxybenzoic acid 4. protocatechualdehyde, 5. p-hydroxybenzoic acid, 6. gentistic acid, 7. chlorogenic acid, 8. vanillic acid + caffeic acid, 9. syringic acid, 10. vanillin, 11. p-coumaric acid + syringaldehyde 12. ferulic acid, 13. sinapic acid, 14. Salicylic

Table 2. The phenolic acid contents (µg/g) of honey samples

Figure 2. UV-Vis spectrum of major phenolic compounds present in various honey samples (a) gallic acid, (b) protocatechuic acid, (c) 2,3,4-trihydroxybenzoic acid, (d) protocatechualdehyde, (e) p-hydroxybenzoic acid, (f) gentistic acid, (g) chlorogenic acid, (h) vanillic acid, (i) syringic acid, (j) vanillin, (k) p-coumaric acid, (l) ferulic acid, (m) sinapic acid, (n) Salicylic acid

Figure 2. UV-Vis spectrum of major phenolic compounds present in various honey samples (a) gallic acid, (b) protocatechuic acid, (c) 2,3,4-trihydroxybenzoic acid, (d) protocatechualdehyde, (e) p-hydroxybenzoic acid, (f) gentistic acid, (g) chlorogenic acid, (h) vanillic acid, (i) syringic acid, (j) vanillin, (k) p-coumaric acid, (l) ferulic acid, (m) sinapic acid, (n) Salicylic acid

Figure 3. High-performance liquid chromatography chromatogram of (a) standard flavonoids solution (100 μg/mL), (b) manuka honey: 1. Hesperitin, 2. Naringin, 3. Hesperidin, 4. Quercitrin, 5. Myricetin, 6. Morin, 7. Luteolin + Quercetin 8. Naringenin, 9. Apigenin, 10.Kaempferol, 11. Chrysin, 12. Galangin, 13.Tangeretin

Figure 3. High-performance liquid chromatography chromatogram of (a) standard flavonoids solution (100 μg/mL), (b) manuka honey: 1. Hesperitin, 2. Naringin, 3. Hesperidin, 4. Quercitrin, 5. Myricetin, 6. Morin, 7. Luteolin + Quercetin 8. Naringenin, 9. Apigenin, 10.Kaempferol, 11. Chrysin, 12. Galangin, 13.Tangeretin

Table 3. The flavonoids content (µg/g) in honey samples

Table 4. Total phenolic acids, total flavonoids and total phenolic compounds in honey samples from different countries

Table 5. Observation of discrimination models and cross-validation results for geographical classification of honey samples based on the phenolic profile

Figure 4. Scatter plot of honey samples (a) from three different regions (b) from four regions

Figure 4. Scatter plot of honey samples (a) from three different regions (b) from four regions