Identification and characterization of flavonoids compounds in cassava leaves (Manihot esculenta Crantz) by HPLC/FTICR-MS

Figures & data

Table 1. Levels of the orthogonal test

Variables		Levels
		1	2	3
Concentration of ethanol (%)	X1	40	50	60
Temperature (°C)	X2	50	60	70
Solid: liquid ratio (g/ml)	X3	20	30	40
Time (h)	X4	1	2	3

Table 2. Results of the orthogonal test

Run	A	B	C	D	TFC (mg/100g dry leaves)
1	1	1	1	1	531.97
2	1	2	2	2	554.36
3	1	3	3	3	682.46
4	2	1	2	3	788.79
5	2	2	3	1	868.38
6	2	3	1	2	877.71
7	3	1	3	2	807.13
8	3	2	1	3	576.12
9	3	3	2	1	780.70

Table 3. Variance analysis of the results of orthogonal test

Source of variation	Sum of squares	Degree of freedom	Mean square	F-value	p-Value
A	1.012	2	0.506	15.333	<0.0001
B	0.206	2	0.103	0.844	>
C	0.244	2	0.122	3.697	<0.0001
D	0.066	2	0.033	0.271	>

Table 4. Compositions and contents of cassava leaves flavonoids

No.	Molecular formula	Formula weight	Structural formula	Retention time (min)	RSD	Relative amount (%)
1	C33H40O20	756		15.9	80178	0.96
2	C27H30O17	626		16.6	403474	4.81
3	C33H40O19	740		17.5	104643	1.25
4	C27H30O16	610		19.5	4943605	58.89
5	C21H20O12	464		21.5	210692	2.51
6	C27H30O15	594		22.7	2460199	29.31
7	C28H32O16	624		23.2	191635	2.28