Figures & data
![](/cms/asset/745c89f2-470e-48f8-8386-2f2dd7dc04e4/ljfp_a_1629690_uf0001_oc.jpg)
Figure 1. Flow chart for the isolation and identification of angiotensin I-converting enzyme (ACE) inhibitory peptides
![Figure 1. Flow chart for the isolation and identification of angiotensin I-converting enzyme (ACE) inhibitory peptides](/cms/asset/bd9d8894-40a8-4e66-b5fa-3d699c7dd6b9/ljfp_a_1629690_f0001_b.gif)
Table 1. Degree of hydrolysis and ACE inhibitory activity of crude peptide extracts derived from pork loin injected with various enzymes
Figure 2. Absorbance at 220 nm and ACE inhibitory activity of gel-filtration fractions derived from thermolysin-injected pork loin (E2). The fractions were divided into four major groups (I–IV), and their ACE inhibitory activity was measured
![Figure 2. Absorbance at 220 nm and ACE inhibitory activity of gel-filtration fractions derived from thermolysin-injected pork loin (E2). The fractions were divided into four major groups (I–IV), and their ACE inhibitory activity was measured](/cms/asset/76ba4a79-79cf-41b4-bc31-7be4cdccbb5d/ljfp_a_1629690_f0002_b.gif)
Figure 3. (a) RP-HPLC profile and (b) ACE inhibitory activity of fraction E2-FIII. Peaks were divided into 17 groups (1–17), and their ACE inhibitory activity was measured
![Figure 3. (a) RP-HPLC profile and (b) ACE inhibitory activity of fraction E2-FIII. Peaks were divided into 17 groups (1–17), and their ACE inhibitory activity was measured](/cms/asset/468e3925-370a-4391-8552-3e136294767e/ljfp_a_1629690_f0003_oc.jpg)
Figure 4. UPLC-QTOF-MS/MS analysis. Peptide with the sequence [(a) Leu-Val-Gly-Arg-Pro-Arg-His-Gly-Gln and (b) Val-Phe-Pro-Ser] of E2-FIII-P7
![Figure 4. UPLC-QTOF-MS/MS analysis. Peptide with the sequence [(a) Leu-Val-Gly-Arg-Pro-Arg-His-Gly-Gln and (b) Val-Phe-Pro-Ser] of E2-FIII-P7](/cms/asset/44c28864-8dd6-4369-b470-b25252596088/ljfp_a_1629690_f0004_oc.jpg)
Table 2. ACE inhibitory activity (IC50) of chemically synthesized peptides identified in thermolysin-injected pork loin