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Original Article

Identification of angiotensin I-converting enzyme inhibitory peptides from enzymatic hydrolysates of pork loin

, , , , &
Pages 1112-1121 | Received 11 Mar 2019, Accepted 02 Jun 2019, Published online: 17 Jun 2019

Figures & data

Figure 1. Flow chart for the isolation and identification of angiotensin I-converting enzyme (ACE) inhibitory peptides

Figure 1. Flow chart for the isolation and identification of angiotensin I-converting enzyme (ACE) inhibitory peptides

Table 1. Degree of hydrolysis and ACE inhibitory activity of crude peptide extracts derived from pork loin injected with various enzymes

Figure 2. Absorbance at 220 nm and ACE inhibitory activity of gel-filtration fractions derived from thermolysin-injected pork loin (E2). The fractions were divided into four major groups (I–IV), and their ACE inhibitory activity was measured

Figure 2. Absorbance at 220 nm and ACE inhibitory activity of gel-filtration fractions derived from thermolysin-injected pork loin (E2). The fractions were divided into four major groups (I–IV), and their ACE inhibitory activity was measured

Figure 3. (a) RP-HPLC profile and (b) ACE inhibitory activity of fraction E2-FIII. Peaks were divided into 17 groups (1–17), and their ACE inhibitory activity was measured

Figure 3. (a) RP-HPLC profile and (b) ACE inhibitory activity of fraction E2-FIII. Peaks were divided into 17 groups (1–17), and their ACE inhibitory activity was measured

Figure 4. UPLC-QTOF-MS/MS analysis. Peptide with the sequence [(a) Leu-Val-Gly-Arg-Pro-Arg-His-Gly-Gln and (b) Val-Phe-Pro-Ser] of E2-FIII-P7

Figure 4. UPLC-QTOF-MS/MS analysis. Peptide with the sequence [(a) Leu-Val-Gly-Arg-Pro-Arg-His-Gly-Gln and (b) Val-Phe-Pro-Ser] of E2-FIII-P7

Table 2. ACE inhibitory activity (IC50) of chemically synthesized peptides identified in thermolysin-injected pork loin