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Research Article

Production of the exopolysaccharide from Lactiplantibacillus plantarum YT013 under different growth conditions: optimum parameters and mathematical analysis

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Pages 1941-1952 | Received 14 Apr 2023, Accepted 16 Jul 2023, Published online: 25 Jul 2023

Figures & data

Table 1. The sources of the chemicals and nutrients.

Figure 1. The influence of culture conditions. Effect of (a) pH, (b) inoculation volume, (c) time, (d) temperature on EPS production.

Figure 1. The influence of culture conditions. Effect of (a) pH, (b) inoculation volume, (c) time, (d) temperature on EPS production.

Figure 2. The influence of medium composition. Effect of (a) carbon source, (b) glucose concentration, (c) nitrogen source, (d) soy peptone concentration, (e) phosphate, (f) KH2PO4 concentration on EPS production.

Figure 2. The influence of medium composition. Effect of (a) carbon source, (b) glucose concentration, (c) nitrogen source, (d) soy peptone concentration, (e) phosphate, (f) KH2PO4 concentration on EPS production.

Table 2. Best-fit nonlinear model equation and correlation factors (R-square) for EPS production.

Figure 3. Fitting results under the best single factor condition. (a) EPS yield of different fermentation time under the best single factor condition; (b) Best-fit nonlinear model equation for EPS production.

Figure 3. Fitting results under the best single factor condition. (a) EPS yield of different fermentation time under the best single factor condition; (b) Best-fit nonlinear model equation for EPS production.

Figure 4. EPS sample and its inhibitory effect on AGS cell proliferation. (a) Fluffy and light-yellow EPS samples were obtained by dialysis and freeze-drying. (b) Cytotoxic effects of crude EPS (400 μg/mL) extracted at different culture times on the human AGS cancer cells. Inhibition effects of crude EPS on the human AGS cancer cells for 24, 48 and 72 h, respectively. (c) control group, (d) treated with EPS (400 μg/mL).

Figure 4. EPS sample and its inhibitory effect on AGS cell proliferation. (a) Fluffy and light-yellow EPS samples were obtained by dialysis and freeze-drying. (b) Cytotoxic effects of crude EPS (400 μg/mL) extracted at different culture times on the human AGS cancer cells. Inhibition effects of crude EPS on the human AGS cancer cells for 24, 48 and 72 h, respectively. (c) control group, (d) treated with EPS (400 μg/mL).

Data availability statement

The authors confirm that the data supporting the findings of this study are available within the article.