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Plant-Insect Interactions

Activity of defense related enzymes and gene expression in pigeon pea (Cajanus cajan) due to feeding of Helicoverpa armigera larvae

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Pages 231-238 | Received 16 Feb 2018, Accepted 15 Apr 2018, Published online: 05 May 2018

Figures & data

Figure 1. Generation of ROS in pigeon pea leaves after 12 h of treatments by DAB staining. Lane C: Control; H: Helicoverpa infested; J: Jasmonic acid sprayed; S: salicylic acid sprayed; E: Ethephon sprayed; M: Mechanically wounded.

Figure 1. Generation of ROS in pigeon pea leaves after 12 h of treatments by DAB staining. Lane C: Control; H: Helicoverpa infested; J: Jasmonic acid sprayed; S: salicylic acid sprayed; E: Ethephon sprayed; M: Mechanically wounded.

Figure 2. Generation of ROS in Helicoverpa infested and un-infested leaves of ICPL-332 and 87 of the same plant after 12 h of Helicoverpa infestation.

Figure 2. Generation of ROS in Helicoverpa infested and un-infested leaves of ICPL-332 and 87 of the same plant after 12 h of Helicoverpa infestation.

Figure 3. Polyphenol oxidase activity in pigeon pea in response to 12 (a) and 24 h (b) treatments. The means ± SE were calculated from data pooled from three experimental repeats.

Figure 3. Polyphenol oxidase activity in pigeon pea in response to 12 (a) and 24 h (b) treatments. The means ± SE were calculated from data pooled from three experimental repeats.

Figure 4. Trypsin inhibitor activity in pigeon pea in response to 12 (a) and 24 h (b) treatments. The means ± SE were calculated from data pooled from three experimental repeats.

Figure 4. Trypsin inhibitor activity in pigeon pea in response to 12 (a) and 24 h (b) treatments. The means ± SE were calculated from data pooled from three experimental repeats.

Figure 5. Levels of expression of defense related transcript in pigeon pea due to feeding on leaves by Helicoverpa larvae and SA, JA, ET treatments. The means ± SE were calculated from data pooled from three experimental repeats. a. Expression of PR-4 (a), Kor-1 (b) and Ltp (c) gene after 12 h of infestation by Helicoverpa larvae and SA, JA, ET treatments in cultivars ICPL-332 and 87; b. Expression of PR-4 (a), Kor-1 (b) and Ltp (c) gene after 12 h of infestation by Helicoverpa larvae and SA, JA, ET treatments in cultivars ICPL-332 and 87.

Figure 5. Levels of expression of defense related transcript in pigeon pea due to feeding on leaves by Helicoverpa larvae and SA, JA, ET treatments. The means ± SE were calculated from data pooled from three experimental repeats. a. Expression of PR-4 (a), Kor-1 (b) and Ltp (c) gene after 12 h of infestation by Helicoverpa larvae and SA, JA, ET treatments in cultivars ICPL-332 and 87; b. Expression of PR-4 (a), Kor-1 (b) and Ltp (c) gene after 12 h of infestation by Helicoverpa larvae and SA, JA, ET treatments in cultivars ICPL-332 and 87.