The effect of soluble saccharides on the activity of key enzymes linked to methyl ketone synthesis in Lactococcus lactis

Figures & data

Figure 1. Effect of soluble saccharides on the growth of L. lactis.

Lowercase letters indicates significantly different between 5 g/L soluble saccharides; capital letters represents significantly different between 20 g/L soluble saccharides (p < 0.05).

Figura 1. Efecto de sacáridos solubles en el crecimiento de L. lactis.

Nota: Las letras minúsculas indican la existencia de diferencias significativas entre sacáridos solubles a 5 g/L; las letras mayúsculas representan diferencias significativas entre sacáridos solubles a 20 g/L (p < 0.05).

Table 1. Effect of soluble saccharides on enzyme activity of β-oxidation pathway (nmol/(mg∙min)).

Tabla 1. Efecto de sacáridos solubles en la actividad enzimática de la ruta de β-oxidación (nmol/(mg∙min)).

	Acyl-CoA dehydrogenase	Enoyl-CoA hydratase	β-Hydroxyacyl-CoA dehydrogenase	Thiolase
Control	9.30 ± 2.27^b	–	119.37 ± 7.34^b	3.54 ± 0.96^a
Glucose	18.95 ± 13.80^b	422.39 ± 234.24^b	53.05 ± 3.19^c	–
Sucrose	15.00 ± 11.03^b	104.11 ± 40.22^b	203.78 ± 35.50^a	3.13 ± 3.80^a
Lactose	–	1172.76 ± 262.53^a	112.94 ± 15.27^b	–
Fructose sucrose	167.67 ± 128.86^a	1320.00 ± 125.06^a	107.72 ± 2.79^b	–
Galactose	–	385.82 ± 45.91^b	123.39 ± 9.13^b	3.96 ± 2.37^a

Different letters in the same column mean significant difference (p < 0.05), ”–” mean not detected.

Las letras distintas en la misma columna representan diferencias significativas (p < 0.05), “–“ indica no detectado.

Table 2. Effect of soluble saccharides on thioesterase activity.

Tabla 2. Efecto de sacáridos solubles en la actividad de la tioesterasa.

	Thioesterase (nmol/(mg∙min))
Control	16.18 ± 0.32^c
Glucose	22.61 ± 0.96^b
Sucrose	19.49 ± 0.64^bc
Lactose	30.52 ± 1.94^a
Fructose	32.72 ± 5.95^a
Galactose	24.63 ± 2.49^b

Different letters in the same column mean significant difference (p < 0.05), “–” mean not detected.

Las letras distintas en la misma columna representan diferencias significativas (p < 0.05), “–” indica no detectado.

Table 3. Pearson correlation analysis between enzyme activity.

Tabla 3. Análisis de correlación de Pearson entre actividades enzimáticas.

	Acyl-CoA dehydrogenase	Enoyl-CoA hydratase	β-Hydroxyacyl-CoA dehydrogenase	Thiolase	Thioesterase
Acyl-CoA dehydrogenase	1	0.516	−0.109	−0.249	0.396
Enoyl-CoA hydratase	0.516	1	−0.341	−0.523	0.826**
β-Hydroxyacyl-CoA dehydrogenase	−0.109	−0.341	1	0.344	−0.304
Thiolase	−0.249	−0.523	0.344	1	−0.423
thioesterase	0.396	0.826**	−0.304	−0.423	1

**Mean significance correlation (p < 0.01).

**Representa correlación significativa (p < 0.01).

Table 4. ANOVA between the thioesterase and enoyl-CoA hydratase activities.

Tabla 4. ANOVA entre las actividades de la tioesterasa y la enoil-CoA hidratasa.

Factor		SS	DF	MS	F	P
Regression	1	2526404.08	1	2526404.08	27.97**	<0.001
Residual standard error	1	1174244.79	13	90326.52
Total	1	3700648.87	14

**Mean significance.

**Representa significativo.