Protective effect of bamboo root dietary Fibre on hyperlipidaemia mice induced by high-fat diet

Figures & data

Table 1. Comparison of animal feeding.

Group	Ingredient percentage/%
	Basic feed	cholesterol	lard	sucrose	Egg yolk powder	Bile salt	Additives
NC	100	—	—	—	—	—	—
HFD	71.6	1.2	10	5	10	0.2	—
PD	71.6	1.2	10	5	10	0.2	2% cholestyramine
LD	71.6	1.2	10	5	10	0.2	2% BRDF
MD	69.6	1.2	10	5	10	0.2	4% BRDF
HD	65.6	1.2	10	5	10	0.2	8% BRDF

NC: Normal Control, HFD: High Fat Diet, PD: Positive Drugs, LD: Low Dietary Fibre, MD: Medium Dietary Fibre, and HD: High Dietary Fibre. The basic feed formula for mice was 30% white flour, 25% corn flour, 20% bran, 15% soybean flour, 4% fish meal, 2% bone meal, 1% egg, 1% yeast powder, 1% salt, and 1% fish liver oil.

Table 2. Body weight changes in the modeled mice.

Date/d	NC (n = 8)	HFD (n = 40)	Weight gain/%
0	20.36 ± 0.31^b	20.43 ± 0.27^b
30	22.99 ± 0.16^a	26.49 ± 0.42^a	15.22

The data is expressed as the mean ± standard deviation, and different letters in the same column indicate significant differences between the data (p < .05). NC: Normal Control, HFD: High Fat Diet, PD: Positive Drugs, LD: Low Dietary Fibre, MD: Medium Dietary Fibre, and HD: High Dietary Fibre.

Table 3. Effect of BRDF on the body weight of mice.

Date/d	NC/g	HFD/g	PD/g	LD/g	MD/g	HD/g
0	22.99 ± 0.16*	25.75 ± 0.37	26.59 ± 0.26	26.65 ± 0.72	26.35 ± 0.31	26.55 ± 0.43
3	23.26 ± 0.20*	26.31 ± 0.36	26.95 ± 0.26	27.31 ± 0.72	27.04 ± 0.30	26.89 ± 0.52
6	23.60 ± 0.20*	27.06 ± 0.38	27.30 ± 0.25	27.94 ± 0.71	27.73 ± 0.32	27.36 ± 0.53
9	23.75 ± 0.18**	27.84 ± 0.37	27.74 ± 0.29	28.61 ± 0.69	28.43 ± 0.35	27.84 ± 0.50
12	24.03 ± 0.17**	28.6 ± 0.34	28.14 ± 0.29	29.28 ± 0.68	29.13 ± 0.32	28.30 ± 0.49
15	24.25 ± 0.19**	29.35 ± 0.34	28.53 ± 0.27*	29.96 ± 0.65	29.60 ± 0.43	28.79 ± 0.47
18	24.53 ± 0.18**	31.14 ± 0.35	28.88 ± 0.24*	30.70 ± 0.64	30.11 ± 0.42	29.28 ± 0.48*
21	24.79 ± 0.17**	31.65 ± 0.29	29.25 ± 0.23*	31.36 ± 0.68	30.63 ± 0.45*	29.69 ± 0.44*
24	25.00 ± 0.19**	32.16 ± 0.48	29.61 ± 0.21**	31.98 ± 0.70	31.34 ± 0.35*	30.15 ± 0.47*
27	25.24 ± 0.18**	32.93 ± 0.52	29.95 ± 0.21**	32.28 ± 0.57*	31.89 ± 0.32*	30.69 ± 0.48*
30	25.53 ± 0.19**	33.71 ± 0.51	3.24 ± 0.20**	32.68 ± 0.42*	32.50 ± 0.33*	31.18 ± 0.51*

Note: The data are expressed as mean ± standard deviation, compared with HFD group, * p <0.05, ** p <0.01. NC: Normal Control, HFD: High Fat Diet, PD: Positive Drugs, LD: Low Dietary Fibre, MD: Medium Dietary Fibre, and HD: High Dietary Fibre.

Table 4. Effect of BRDF on the intake of mice.

Date/d	NC/g	HFD/g	PD/g	LD/g	MD/g	HD/g
0	3.70 ± 0.17	3.53 ± 0.35	3.55 ± 0.21	3.44 ± 0.25	3.35 ± 0.21	3.28 ± 0.29
3	3.56 ± 0.67	3.46 ± 0.38	3.54 ± 0.18	3.21 ± 0.31	3.35 ± 0.30	3.21 ± 0.25
6	3.34 ± 0.24	3.41 ± 0.35	3.54 ± 0.34	3.30 ± 0.41	3.35 ± 0.30	3.26 ± 0.28
9	3.45 ± 0.38	3.44 ± 0.42	3.46 ± 0.34	3.53 ± 0.48	3.44 ± 0.27	3.35 ± 0.42
12	3.24 ± 0.37	3.56 ± 0.30	3.18 ± 0.60	3.55 ± 0.24	3.29 ± 0.28	3.40 ± 0.35
15	3.41 ± 0.74	3.51 ± 0.34	3.53 ± 0.29	3.53 ± 0.21	3.21 ± 0.29	3.44 ± 0.21
18	3.39 ± 0.57	3.43 ± 0.41	3.36 ± 0.33	3.45 ± 0.31	3.35 ± 0.37	3.38 ± 0.21
21	3.46 ± 0.29	3.43 ± 0.40	3.38 ± 0.32	3.43 ± 0.44	3.30 ± 0.31	3.38 ± 0.23
24	3.39 ± 0.31	3.44 ± 0.38	3.38 ± 0.41	3.34 ± 0.32	3.61 ± 0.26	3.26 ± 0.27
27	3.31 ± 0.50	3.51 ± 0.29	3.33 ± 0.34	3.49 ± 0.38	3.40 ± 0.24	3.33 ± 0.28
30	3.55 ± 0.25	3.43 ± 0.41	3.40 ± 0.40	3.38 ± 0.31	3.54 ± 0.33	3.38 ± 0.24

NC: Normal Control, HFD: High Fat Diet, PD: Positive Drugs, LD: Low Dietary Fibre, MD: Medium Dietaryf Fibre, and HD: High Dietary Fibre.

Figure 1. Effect of BRDF on the levels of serum lipids in mice.

Figure 2. Effect of BRDF on the levels of AI in mice.

Table 5. Effect of BRDF on liver weight and liver index of mice.

Group	Liver weight/g	Liver index
NC	1.13 ± 0.07^b	4.42 ± 0.27^b
HFD	1.80 ± 0.06a	5.33 ± 0.21a
PD	1.35 ± 0.04^b	4.46 ± 0.11^b
HD	1.42 ± 0.03^b	4.56 ± 0.15^b
MD	1.63 ± 0.06^b	5.01 ± 0.24^b
LD	1.69 ± 0.09^b	5.10 ± 0.32^b

The data is expressed as the mean ± standard deviation, and different letters in the same column indicate significant differences between the data (p < .05). NC: Normal Control, HFD: High Fat Diet, PD: Positive Drugs, LD: Low Dietary Fibre, MD: Medium Dietary Fibre, and HD: High Dietary Fibre.

Figure 3. Effect of BRDF on the TC and TG content in mice liver.

Figure 4. Liver histological image of mice. (The black arrow represents lipid droplet vacuoles of varying sizes within liver cells. The blue arrow represents an inflammatory cell lesion found in the liver tissue.).