Determination of pentachlorophenol and its sodium salt residue in eggs using d-SPE method coupled with UPLC-MS/MS

Figures & data

Table 1. Mobile phase conditions of LC for PCP&Na-PCP.

Time (min)	Mobile phase A (%)	Mobile phase B (%)	Flow (mL/min)
0	40	60	0.25
1	100	0	0.25
7	100	0	0.25
7.5	40	60	0.25
9	40	60	0.25

Mobile phase A: MeoH; Mobile phase B: 1% acetic acid aqueous solution (V/V).

Table 2. MS conditions for PCP&Na-PCP.

Precursor ions (m/z)	Product ions (m/z)	Dwell time (msec)	Q1 pre bias (V)	CE (V)	Q3 pre bias (V)
262.7	262.7	100	13	23	16
264.7	264.7	100	12	23	11
266.7	266.7	100	18	24	16
268.7	268.7	100	13	23	11

Figure 1. Comparison of the chromatographic peak shapes at different flow rates.

Figure 2. The TIC of egg extraction was cleaned by different purification sorbent (a: Matrix without cleanup; b: Matrix cleanup with 400 mg PSA; c: Matrix cleanup with 400 mg C18; d: Matrix cleanup with 200 mg PSA and 200 mg C18).

Table 3. Recovery (%), relative standard deviation (RSD, %), r² and LOQ of egg spiked with PCP&Na-PCP.

Compound	Spiked level (μg/kg)	Recovery (%)	RSD (%)	r^2	LOQ (μg/kg)
PCP	0.5	72.2	0.2	0.9999	0.5
	5	83.1	0.8
	50	89.8	0.5

Data availability statement

The data presented in this study are available on request from the corresponding author.