Figures & data
Figure 1. Deletion of stk1 leads to reduced production of PCN and PVD, and attenuated twitching motility.
![Figure 1. Deletion of stk1 leads to reduced production of PCN and PVD, and attenuated twitching motility.](/cms/asset/38937d57-47dc-47b4-9802-e92875d44532/kvir_a_2367649_f0001_oc.jpg)
Figure 2. Effect of the deletion of stk1 on biofilm production and EPS secretion.
![Figure 2. Effect of the deletion of stk1 on biofilm production and EPS secretion.](/cms/asset/d3950cd1-87d0-46bb-a092-7b118842e950/kvir_a_2367649_f0002_oc.jpg)
Table 1. MIC of the indicated antibiotics for the strains PAO1, Δstk1, Δstk1:stk1 and PAO1-algRS143A.
Figure 3. AlgR is a substrate of Stk1 and its Ser143 can be phosphorylated by this kinase.
![Figure 3. AlgR is a substrate of Stk1 and its Ser143 can be phosphorylated by this kinase.](/cms/asset/fc31829b-4708-4258-9e65-49351c8dae95/kvir_a_2367649_f0003_oc.jpg)
Figure 4. Both the deletion of stk1 and the mutation of algRS143A led to increased biofilm production, EPS secretion and antibiotic resistance.
![Figure 4. Both the deletion of stk1 and the mutation of algRS143A led to increased biofilm production, EPS secretion and antibiotic resistance.](/cms/asset/f7354d07-8094-4e08-8483-0bfa761b772f/kvir_a_2367649_f0004_oc.jpg)
Figure 5. Deletion of stk1 and mutation of algRS143A both resulted in decreased PCN production and attenuated twitching motility.
![Figure 5. Deletion of stk1 and mutation of algRS143A both resulted in decreased PCN production and attenuated twitching motility.](/cms/asset/5a5cfb01-7174-429a-8d70-532db9ae9382/kvir_a_2367649_f0005_oc.jpg)
Figure 6. Determination of the relative expression of algR and several algR-regulated genes in PAO1, Δstk1 and PAO1-algRS143A by qPCR.
![Figure 6. Determination of the relative expression of algR and several algR-regulated genes in PAO1, Δstk1 and PAO1-algRS143A by qPCR.](/cms/asset/f895dba4-6f1f-445b-9e74-892d688b5a9a/kvir_a_2367649_f0006_oc.jpg)
Supplemental Material
Download MS Word (566.7 KB)Data availability statement
The data that support the findings of this study are openly available in figshare at https://doi.org/10.6084/m9.figshare.25551474.v3, reference number 25,551,474.