Figures & data
Figure 1. Lixisenatide ameliorates advanced glycation end products (AGEs)-induced mitochondrial dysfunction. (A) Mitochondrial membrane potential was measured by TMRM; (B) Intracellular ATP levels were determined by a luciferase assay (*, #, $, p < .01).
![Figure 1. Lixisenatide ameliorates advanced glycation end products (AGEs)-induced mitochondrial dysfunction. (A) Mitochondrial membrane potential was measured by TMRM; (B) Intracellular ATP levels were determined by a luciferase assay (*, #, $, p < .01).](/cms/asset/2f1ae870-09c4-480d-a75b-3dbd6dbebdc4/ianb_a_1593996_f0001_c.jpg)
Figure 2. Lixisenatide ameliorates advanced glycation end products (AGEs)-induced oxidative stress. (A) The lipid peroxidation 4-HNE was determined by immunostaining; (B). NOX-4 was determined by western blot analysis (*, #, $, p < .01).
![Figure 2. Lixisenatide ameliorates advanced glycation end products (AGEs)-induced oxidative stress. (A) The lipid peroxidation 4-HNE was determined by immunostaining; (B). NOX-4 was determined by western blot analysis (*, #, $, p < .01).](/cms/asset/a7ba2fbb-dc05-42ae-b4b2-56d26bc7aa7c/ianb_a_1593996_f0002_c.jpg)
Figure 3. Lixisenatide ameliorates advanced glycation end products (AGEs)-induced expression of MMP-3 and MMP-13. (A) Expression of MMP-3 and MMP-13 determined by real-time PCR analysis; (B) Expression of MMP-3 and MMP-13 determined by western blot analysis (*, #, $, p < .01).
![Figure 3. Lixisenatide ameliorates advanced glycation end products (AGEs)-induced expression of MMP-3 and MMP-13. (A) Expression of MMP-3 and MMP-13 determined by real-time PCR analysis; (B) Expression of MMP-3 and MMP-13 determined by western blot analysis (*, #, $, p < .01).](/cms/asset/87bee8a2-3c0f-4306-a8d2-73a75df7ffcb/ianb_a_1593996_f0003_b.jpg)
Figure 4. Lixisenatide ameliorates advanced glycation end products (AGEs)-induced degradation of type II collagen. Type II collagen was determined by western blot analysis (*, #, $, p < .01).
![Figure 4. Lixisenatide ameliorates advanced glycation end products (AGEs)-induced degradation of type II collagen. Type II collagen was determined by western blot analysis (*, #, $, p < .01).](/cms/asset/b1be4731-50af-4fb4-98ce-4ef87ab3d51f/ianb_a_1593996_f0004_b.jpg)
Figure 5. Lixisenatide ameliorates advanced glycation end products (AGEs)-induced expression of ADAMTS-4 and ADAMTS-5. (A) Expression of ADAMTS-4 and ADAMTS-5 were determined by real-time PCR analysis; (B) Expression of ADAMTS-4 and ADAMTS-5 were determined by western blot analysis (*, #, $, p < .01).
![Figure 5. Lixisenatide ameliorates advanced glycation end products (AGEs)-induced expression of ADAMTS-4 and ADAMTS-5. (A) Expression of ADAMTS-4 and ADAMTS-5 were determined by real-time PCR analysis; (B) Expression of ADAMTS-4 and ADAMTS-5 were determined by western blot analysis (*, #, $, p < .01).](/cms/asset/e6fc2168-686f-4db2-b39d-51bdce1ff9bd/ianb_a_1593996_f0005_b.jpg)
Figure 6. Lixisenatide ameliorates advanced glycation end products (AGEs)-induced degradation of aggrecan. Aggrecan was determined by western blot analysis (*, #, $, p < .01).
![Figure 6. Lixisenatide ameliorates advanced glycation end products (AGEs)-induced degradation of aggrecan. Aggrecan was determined by western blot analysis (*, #, $, p < .01).](/cms/asset/300dd560-a59d-420b-806c-b2a7588e1813/ianb_a_1593996_f0006_b.jpg)
Figure 7. Lixisenatide ameliorates advanced glycation end products (AGEs)-induced secretion of TNF-α and IL-6. (A) Expression of TNF-α and IL-6 at the gene level was determined real-time PCR analysis; (B) Secretion of TNF-α and IL-6 at the protein level was determined ELISA (*, #, $, p < .01).
![Figure 7. Lixisenatide ameliorates advanced glycation end products (AGEs)-induced secretion of TNF-α and IL-6. (A) Expression of TNF-α and IL-6 at the gene level was determined real-time PCR analysis; (B) Secretion of TNF-α and IL-6 at the protein level was determined ELISA (*, #, $, p < .01).](/cms/asset/ae190def-9f76-4156-be95-d34d446c0bfd/ianb_a_1593996_f0007_b.jpg)