Figures & data
Figure 1. Expression levels of miR-27b-3p and HIPK2 in cartilage tissues from RA and NC patients. (A) The H&E staining demonstrated the pathological features of RA.The expression of (B) miR-27b-3p and (C) HIPK2 mRNA in cartilage tissues. (D) The correlation between miR-27b-3p and HIPK2 mRNA expression in RA cartilage tissues. (E,F) The expression of HIPK2 protein in RA cartilage tissues. The normal articular cartilage tissues were served as control. **p < .01.
Figure 2. MicroRNA-27b-3p regulated HIPK2. (A) The regulation between miR-27b-3p and HIPK2 was evaluated using dual-luciferase reporter gene assays. (B) MiR-27b-3p regulated the protein expression of HIPK2.
Figure 3. Recombinant HIPK2 antagonized miR-27b-3p mimic-inhibited cell apoptosis. (A–F) The protein level of Bax, Bcl2, cleaved caspase-3 and cytochrome determined by Western blotting. (G) Percentage of apoptotic cells measured after treatment with miR-27b-3p mimic or combination of HIPK2 and miR-27b-3p inhibitor.
Availability of data and materials
The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.