Figures & data
Figure 1. Geniposide attenuates PC12 cells viability loss and apoptosis induced by LPS. PC12 cells viability was examined by CCK-8 assay, (A) after the cells were treated by various does of geniposide alone, (B) or in combination with LPS. (C) Apoptosis rate and (D) the accumulation of proteins involved in apoptosis were detected by flow cytometry and Western blot respectively, after treating with LPS or LPS plus geniposide. (E) Semi-quantitation of relative protein accumulation based on the results of Western blot. ns: no significance; *, p < .05; **, p < .01; ***, p < .001.
Figure 2. Geniposide ameliorates the release of pro-inflammatory cytokines evoked by LPS. PC12 cells were treated by LPS or LPS plus geniposide. (A) The mRNA and (B) protein levels of pro-inflammatory cytokines, as well as (C) their concentrations in the culture supernatant were respectively measured by RT-qPCR, Western blot and ELISA. *, p < .05; **, p < .01; ***, p < .001.
Figure 3. Geniposide elevates miR-145-5p expression. PC12 cells were treated by LPS or LPS plus geniposide. miR-145-5p expression was examined by RT-qPCR. *, p < .05; ***, p < .001.
Figure 4. Geniposide protects PC12 cells against LPS-evoked cell damage via miR-145-5p. (A) miR-145-5p expression was examined by RT-qPCR after PC12 cells were transfected with inhibitor or NC specific for miR-145-5p. The transfected cells were then treated by LPS or LPS plus geniposide. (B) Cell viability, (C) apoptosis rate, (D,E) accumulation of proteins involved in apoptosis, (F) mRNA levels of cytokines, (G) protein levels of cytokines and (H) the release of cytokines were tested using CCK-8 assay, flow cytometry, RT-qPCR, Western blot and ELISA. *, p < .05; **, p < .01; ***, p < .001.
Figure 5. Geniposide blocks NF-κB and JNK pathways via miR-145-5p. PC12 cells were transfected with inhibitor or NC specific for miR-145-5p, after which the cells were treated by LPS or LPS plus geniposide. Phosphorylation of (A,B) IκBα, p65 and (C,D) JNK was examined using Western blot. *, p < .05; **, p < .01; ***, p < .001.
